Calculator
Tide Fluor™ 2 amine [TF2 amine] *Superior replacement for fluorescein*
Tide Fluor™ 2 (TF2) family has the spectral properties similar to those of fluoresceins and Alexa Fluor® 488 (Alexa Fluor® is the trademark of Invitrogen). Compared to fluoresceins TF2 family has much stronger fluorescence and higher photostability. Additionally their fluorescence is pH-independent from pH 3 to 11. These characteristics make this new dye family a superior alternative to fluoresceins. TF2-labeled peptides and nucleotides exhibit much stronger fluorescence and higher photostability than the ones labeled with FITC or FAM. In pairing with our Tide Quencher™ 2 (TQ2), a variety of FRET peptides and nucleotides can be developed for detecting proteases and molecular beacons with enhanced sensitivity and stability. In addition, TF2 has spectral properties, pH dependence and photostability similar to Alexa Fluor® 488, making them excellent replacement for the expensive Alexa Fluor® 488. This TF2 product is primarily used for labeling the small molecules that contain a carbonyl group.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Fluor™ 2 amine [TF2 amine] *Superior replacement for fluorescein* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 131.991 µL | 659.953 µL | 1.32 mL | 6.6 mL | 13.199 mL |
| 5 mM | 26.398 µL | 131.991 µL | 263.981 µL | 1.32 mL | 2.64 mL |
| 10 mM | 13.199 µL | 65.995 µL | 131.991 µL | 659.953 µL | 1.32 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (280 nm) |
| Tide Fluor™ 1 amine [TF1 amine] *Superior replacement for EDANS* | 341 | 447 | 20000 | 0.911 | 0.187 |
| Tide Fluor™ 2 azide [TF2 azide] | 503 | 525 | 75000 | 0.91 | 0.09 |
| Tide Fluor™ 2 alkyne [TF2 alkyne] | 503 | 525 | 75000 | 0.91 | 0.09 |
| Tide Fluor™ 3 amine [TF3 amine] *Superior replacement for Cy3* | 553 | 578 | 750001 | 0.11 | 0.179 |
| Tide Fluor™ 5WS amine [TF5WS amine] *Superior replacement for Cy5* | 649 | 663 | 250000 | 0.271 | 0.027 |
| Tide Fluor™ 4 amine [TF4 amine] *Superior replacement for ROX and Texas Red* | 577 | 602 | 90000 | 0.771 | 0.436 |
| Tide Fluor™ 6WS amine [TF6WS amine] *Superior replacement for Cy5.5* | 682 | 701 | 220000 | 0.271 | 0.101 |
| Tide Fluor™ 7WS amine [TF7WS amine] *Superior replacement for Cy7* | 756 | 780 | 275000 | 0.31 | 0.049 |
| Tide Fluor™ 8WS amine [TF8WS amine] *Near Infrared Emission* | 785 | 801 | 250000 | 0.211 | 0.109 |
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Citations
View all 8 citations: Citation Explorer
Intraocular Delivery of a Collagen Mimetic Peptide Repairs Retinal Ganglion Cell Axons in Chronic and Acute Injury Models
Authors: Ribeiro, Marcio and McGrady, Nolan R and Baratta, Robert O and Del Buono, Brian J and Schlumpf, Eric and Calkins, David J
Journal: International Journal of Molecular Sciences (2022): 2911
Authors: Ribeiro, Marcio and McGrady, Nolan R and Baratta, Robert O and Del Buono, Brian J and Schlumpf, Eric and Calkins, David J
Journal: International Journal of Molecular Sciences (2022): 2911
A mechanistic model to predict effects of cathepsin B and cystatin C on β-amyloid aggregation and degradation
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Real-Time Detection of a Self-Replicating RNA Enzyme
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310
Maternal serum glycosylated fibronectin as a point-of-care biomarker for assessment of preeclampsia
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1
Development of Multi-Parametric/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16
References
View all 25 references: Citation Explorer
Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288
Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202
Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer
Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147
Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147
The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates
Authors: Kim GI, Kim KW, Oh MK, Sung YM.
Journal: Nanotechnology (2009): 175503
Authors: Kim GI, Kim KW, Oh MK, Sung YM.
Journal: Nanotechnology (2009): 175503
Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening
Authors: Yu X, Sainz B, Jr., Uprichard SL.
Journal: Antimicrob Agents Chemother (2009): 4311
Authors: Yu X, Sainz B, Jr., Uprichard SL.
Journal: Antimicrob Agents Chemother (2009): 4311
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