Rhod-4™ azide

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	881.90
Solvent	DMSO

Spectral properties

Excitation (nm)	523
Emission (nm)	551
Quantum yield	0.1¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Alternative formats

Overview SDS Protocol

Molecular weight

881.90

Excitation (nm)

523

Emission (nm)

551

Quantum yield

0.1¹

Rhod-4™ azide is an excellent building block that can be readily used to prepare a calcium-sensitive bioconjugate for monitoring calcium change spatially for a specific target. It can be conjugated to alkyne-containing biomolecules via the well-known click chemistry. Rhod-4™ is a fluorescent calcium indicator dye commonly used in cell biology and neuroscience research. It belongs to the family of rhodol-based calcium. Rhod-4™ is a high-affinity calcium indicator dye that exhibits changes in fluorescence intensity upon binding to calcium ions. When Rhod-4™ binds to calcium, its fluorescence intensity increases dramatically, allowing researchers to measure and monitor changes in calcium levels. Compared to the traditional Rhod-2, Rhod-4™ is a superior replacement. It is a valuable tool in calcium imaging research, enabling the detection and quantification of intracellular calcium dynamics. It's fluorescence properties and calcium binding specificity make it suitable for studying calcium signaling in various cell types and biological systems.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Rhod-4™ azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	113.392 µL	566.958 µL	1.134 mL	5.67 mL	11.339 mL
5 mM	22.678 µL	113.392 µL	226.783 µL	1.134 mL	2.268 mL
10 mM	11.339 µL	56.696 µL	113.392 µL	566.958 µL	1.134 mL

Molarity calculator

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Spectrum

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Spectral properties

Excitation (nm)	523
Emission (nm)	551
Quantum yield	0.1¹

Product Family

Name	Excitation (nm)	Emission (nm)	Quantum yield
Rhod-4™, AM	523	551	0.1¹

Images

Figure 1. Rhod-4™ azide is an excellent building block that can be readily used to prepare a calcium-sensitive bioconjugate for monitoring calcium change spatially for a specific target. It can be conjugated to alkyne-containing biomolecule via the well-known click chemistry.

References

View all 50 references: Citation Explorer

Mitochondria-Endoplasmic Reticulum Contact Sites Dynamics and Calcium Homeostasis Are Differentially Disrupted in PINK1-PD or PRKN-PD Neurons.
Authors: Grossmann, Dajana and Malburg, Nina and Glaß, Hannes and Weeren, Veronika and Sondermann, Verena and Pfeiffer, Julia F and Petters, Janine and Lukas, Jan and Seibler, Philip and Klein, Christine and Grünewald, Anne and Hermann, Andreas
Journal: Movement disorders : official journal of the Movement Disorder Society (2023)

Cardiomyocyte Targeting Peptide to Deliver Amiodarone.
Authors: Zahid, Maliha and Weber, Beth and Yurko, Ray and Islam, Kazi and Agrawal, Vaishavi and Lopuszynski, Jack and Yagi, Hisato and Salama, Guy
Journal: bioRxiv : the preprint server for biology (2023)

Palmitic acid methyl ester induces cardiac hypertrophy through activating the GPR receptor-mediated changes of intracellular calcium concentrations and mitochondrial functions.
Authors: Lien, Chih-Feng and Chiu, Hung-Wen and Lee, Wen-Sen and Lin, Jian-Hong and Wang, Yi-Shun and Ting, Pei-Ching and Luo, Yu-Po and Chang, Jui-Chih and Yang, Kun-Ta
Journal: Journal of cellular physiology (2023): 242-256

CDN1163, a SERCA activator, causes intracellular Ca2+ leak, mitochondrial hyperpolarization and cell cycle arrest in mouse neuronal N2A cells.
Authors: Huang, Yi-Ping and Huang, Wen-Wei and Tsai, Kun-Feng and Shiao, Lian-Ru and Yang, Zih-He and Tseng, Shao-Yun and Lin, Yu-Hsien and Chen, Cing-Yu and Chan, Paul and Leung, Yuk-Man
Journal: Neurotoxicology (2023): 9-15

Piezo1 channel activation stimulates ATP production through enhancing mitochondrial respiration and glycolysis in vascular endothelial cells.
Authors: Jiang, Man and Zhang, Yi-Xin and Bu, Wen-Jie and Li, Ping and Chen, Jia-Hui and Cao, Ming and Dong, Yan-Chao and Sun, Zhi-Jie and Dong, De-Li
Journal: British journal of pharmacology (2023): 1862-1877

Comparing the effects of chemical Ca2+ dyes and R-GECO on contractility and Ca2+ transients in adult and human iPSC cardiomyocytes.
Authors: Robinson, Paul and Sparrow, Alexander J and Psaras, Yiangos and Steeples, Violetta and Simon, Jillian N and Broyles, Connor N and Chang, Yu-Fen and Brook, Frances A and Wang, Ying-Jie and Blease, Andrew and Zhang, Xiaoyu and Abassi, Yama A and Geeves, Michael A and Toepfer, Christopher N and Watkins, Hugh and Redwood, Charles and Daniels, Matthew J
Journal: Journal of molecular and cellular cardiology (2023): 44-57

Mitochondrial connexin43 and mitochondrial KATP channels modulate triggered arrhythmias in mouse ventricular muscle.
Authors: Sato, Haruka and Nishiyama, Masami and Morita, Natsuki and Satoh, Wakako and Hasegawa, Taiki and Someya, Yuka and Okumura, Tsuyoshi and Koyama, Sana and Shindoh, Chiyohiko and Miura, Masahito
Journal: Pflugers Archiv : European journal of physiology (2023): 477-488

Autonomic Regulation of the Goldfish Intact Heart.
Authors: Bazmi, Maedeh and Escobar, Ariel L
Journal: Frontiers in physiology (2022): 793305

Inhibition of mitochondrial reactive oxygen species improves coronary endothelial function after cardioplegic hypoxia/reoxygenation.
Authors: Song, Yi and Xing, Hang and He, Yixin and Zhang, Zhiqi and Shi, Guangbin and Wu, Su and Liu, Yuhong and Harrington, Elizabeth O and Sellke, Frank W and Feng, Jun
Journal: The Journal of thoracic and cardiovascular surgery (2022): e207-e226

Decabromodiphenyl ether initiates mitochondria-dependent apoptosis by disrupting calcium homeostasis in mice livers.
Authors: Che, Siyan and Chen, Sunni and Li, Shiqi and Ruan, Zheng
Journal: Chemosphere (2022): 132767