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PUR-1
PUR-1 is essentially non-fluorescent in the absence of nucleic acids. Its fluorescence is significantly enhanced in the presence of DNA and/or RNA. In the presence of RNA, PUR-1 has fluorescence at ~478 nm with fluorescence quantum yield of ~40%. PUR-1 dye is excitable at 488 nm (with maximal excitation at 460 nm) and emits fluorescence between 470 to 550 nm with a maximum emission at 478 nm in the presence of nucleic acids. The dye can be used for analyzing blood-borne parasite infections in combination with a flow cytometer.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of PUR-1 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 228.676 µL | 1.143 mL | 2.287 mL | 11.434 mL | 22.868 mL |
| 5 mM | 45.735 µL | 228.676 µL | 457.352 µL | 2.287 mL | 4.574 mL |
| 10 mM | 22.868 µL | 114.338 µL | 228.676 µL | 1.143 mL | 2.287 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield |
| Oxazine 1 *CAS 24796-94-9* | 648 | 668 | 123000 | 0.11001 |
| DiTO™-1 [equivalent to TOTO®-1] *5 mM DMSO Solution* *CAS 143413-84-7* | 514 | 531 | 1170001 | 0.341 |
| DiYO™-1 [equivalent to YOYO®-1] *5 mM DMSO Solution* | 491 | 508 | 989001 | 0.521 |
| JC-1 [5,5,6,6-Tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide] *CAS#: 3520-43-2* | 515 | 530 | 1950001 | - |
| BTA-1 [2-(4'-(methylamino)phenyl)benzothiazole] | 349 | 421 | - | - |
| DiYO™-1 [equivalent to YOYO®-1] | 491 | 508 | 989001 | 0.521 |
| DiPO™-1 [equivalent to POPO®-1] *1 mM DMSO Solution* | 433 | 457 | 920001 | 0.61 |
| EthD-1 [Ethidium Homodimer-1] | 528 | 617 | - | - |
References
View all 52 references: Citation Explorer
Inhibition of Mitochondrial Fission Protein Reduced Mechanical Allodynia and Suppressed Spinal Mitochondrial Superoxide Induced by Perineural Human Immunodeficiency Virus gp120 in Rats
Authors: K, undefined and a H, Liu S, Iida T, Yi H, Huang W, Levitt RC, Lubarsky DA, C and iotti KA, Hao S.
Journal: Anesth Analg (2016): 264
Authors: K, undefined and a H, Liu S, Iida T, Yi H, Huang W, Levitt RC, Lubarsky DA, C and iotti KA, Hao S.
Journal: Anesth Analg (2016): 264
Up-Regulation of Mitochondrial Antioxidant Superoxide Dismutase Underpins Persistent Cardiac Nutritional-Preconditioning by Long Chain n-3 Polyunsaturated Fatty Acids in the Rat
Authors: Abdukeyum GG, Owen AJ, Larkin TA, McLennan PL.
Journal: J Clin Med (2016): 32
Authors: Abdukeyum GG, Owen AJ, Larkin TA, McLennan PL.
Journal: J Clin Med (2016): 32
Mitochondrial flashes: dump superoxide and dance with protons now
Authors: Demaurex N, Schwarzl and er M., undefined
Journal: Antioxid Redox Signal. (2016)
Authors: Demaurex N, Schwarzl and er M., undefined
Journal: Antioxid Redox Signal. (2016)
Expression and polymorphism (rs4880) of mitochondrial superoxide dismutase (SOD2) and asparaginase induced hepatotoxicity in adult patients with acute lymphoblastic leukemia
Authors: Alachkar H, Fulton N, Sanford B, Malnassy G, Mutonga M, Larson RA, Bloomfield CD, Marcucci G, Nakamura Y, Stock W.
Journal: Pharmacogenomics J. (2016)
Authors: Alachkar H, Fulton N, Sanford B, Malnassy G, Mutonga M, Larson RA, Bloomfield CD, Marcucci G, Nakamura Y, Stock W.
Journal: Pharmacogenomics J. (2016)
Adipocyte-Specific Deletion of Manganese Superoxide Dismutase Protects From Diet-Induced Obesity Through Increased Mitochondrial Uncoupling and Biogenesis
Authors: Han YH, Buffolo M, Pires KM, Pei S, Scherer PE, Boudina S.
Journal: Diabetes (2016): 2639
Authors: Han YH, Buffolo M, Pires KM, Pei S, Scherer PE, Boudina S.
Journal: Diabetes (2016): 2639
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