PUR-1
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 437.30 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H340 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R68 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 41116134 |
Overview | SDSProtocol |
See also: Nucleus
Molecular weight 437.30 |
PUR-1 is essentially non-fluorescent in the absence of nucleic acids. Its fluorescence is significantly enhanced in the presence of DNA and/or RNA. In the presence of RNA, PUR-1 has fluorescence at ~478 nm with fluorescence quantum yield of ~40%. PUR-1 dye is excitable at 488 nm (with maximal excitation at 460 nm) and emits fluorescence between 470 to 550 nm with a maximum emission at 478 nm in the presence of nucleic acids. The dye can be used for analyzing blood-borne parasite infections in combination with a flow cytometer.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of PUR-1 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 228.676 µL | 1.143 mL | 2.287 mL | 11.434 mL | 22.868 mL |
5 mM | 45.735 µL | 228.676 µL | 457.352 µL | 2.287 mL | 4.574 mL |
10 mM | 22.868 µL | 114.338 µL | 228.676 µL | 1.143 mL | 2.287 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield |
Oxazine 1 *CAS 24796-94-9* | 648 | 668 | 123000 | 0.11001 |
DiTO™-1 [equivalent to TOTO®-1] *5 mM DMSO Solution* *CAS 143413-84-7* | 514 | 531 | 1170001 | 0.341 |
DiYO™-1 [equivalent to YOYO®-1] *5 mM DMSO Solution* | 491 | 508 | 989001 | 0.521 |
JC-1 [5,5,6,6-Tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide] *CAS#: 3520-43-2* | 515 | 530 | 1950001 | - |
BTA-1 [2-(4'-(methylamino)phenyl)benzothiazole] | 349 | 421 | - | - |
DiYO™-1 [equivalent to YOYO®-1] | 491 | 508 | 989001 | 0.521 |
DiPO™-1 [equivalent to POPO®-1] *1 mM DMSO Solution* | 433 | 457 | 920001 | 0.61 |
EthD-1 [Ethidium Homodimer-1] | 528 | 617 | - | - |
References
View all 52 references: Citation Explorer
Inhibition of Mitochondrial Fission Protein Reduced Mechanical Allodynia and Suppressed Spinal Mitochondrial Superoxide Induced by Perineural Human Immunodeficiency Virus gp120 in Rats
Authors: K, undefined and a H, Liu S, Iida T, Yi H, Huang W, Levitt RC, Lubarsky DA, C and iotti KA, Hao S.
Journal: Anesth Analg (2016): 264
Authors: K, undefined and a H, Liu S, Iida T, Yi H, Huang W, Levitt RC, Lubarsky DA, C and iotti KA, Hao S.
Journal: Anesth Analg (2016): 264
Up-Regulation of Mitochondrial Antioxidant Superoxide Dismutase Underpins Persistent Cardiac Nutritional-Preconditioning by Long Chain n-3 Polyunsaturated Fatty Acids in the Rat
Authors: Abdukeyum GG, Owen AJ, Larkin TA, McLennan PL.
Journal: J Clin Med (2016): 32
Authors: Abdukeyum GG, Owen AJ, Larkin TA, McLennan PL.
Journal: J Clin Med (2016): 32
Mitochondrial flashes: dump superoxide and dance with protons now
Authors: Demaurex N, Schwarzl and er M., undefined
Journal: Antioxid Redox Signal. (2016)
Authors: Demaurex N, Schwarzl and er M., undefined
Journal: Antioxid Redox Signal. (2016)
Expression and polymorphism (rs4880) of mitochondrial superoxide dismutase (SOD2) and asparaginase induced hepatotoxicity in adult patients with acute lymphoblastic leukemia
Authors: Alachkar H, Fulton N, Sanford B, Malnassy G, Mutonga M, Larson RA, Bloomfield CD, Marcucci G, Nakamura Y, Stock W.
Journal: Pharmacogenomics J. (2016)
Authors: Alachkar H, Fulton N, Sanford B, Malnassy G, Mutonga M, Larson RA, Bloomfield CD, Marcucci G, Nakamura Y, Stock W.
Journal: Pharmacogenomics J. (2016)
Adipocyte-Specific Deletion of Manganese Superoxide Dismutase Protects From Diet-Induced Obesity Through Increased Mitochondrial Uncoupling and Biogenesis
Authors: Han YH, Buffolo M, Pires KM, Pei S, Scherer PE, Boudina S.
Journal: Diabetes (2016): 2639
Authors: Han YH, Buffolo M, Pires KM, Pei S, Scherer PE, Boudina S.
Journal: Diabetes (2016): 2639
Mitochondrial generation of superoxide and hydrogen peroxide as the source of mitochondrial redox signaling
Authors: Br, undefined and MD., undefined
Journal: Free Radic Biol Med. (2016)
Authors: Br, undefined and MD., undefined
Journal: Free Radic Biol Med. (2016)
Near-Infrared Fluorescence Probe for in Situ Detection of Superoxide Anion and Hydrogen Polysulfides in Mitochondrial Oxidative Stress
Authors: Huang Y, Yu F, Wang J, Chen L.
Journal: Anal Chem (2016): 4122
Authors: Huang Y, Yu F, Wang J, Chen L.
Journal: Anal Chem (2016): 4122
Potential role of mitochondrial superoxide decreasing ferrochelatase and heme in coronary artery soluble guanylate cyclase depletion by angiotensin II
Authors: Patel D, Alhawaj R, Kelly MR, Accarino JJ, Lakhkar A, Gupte SA, Sun D, Wolin MS.
Journal: Am J Physiol Heart Circ Physiol (2016): H1439
Authors: Patel D, Alhawaj R, Kelly MR, Accarino JJ, Lakhkar A, Gupte SA, Sun D, Wolin MS.
Journal: Am J Physiol Heart Circ Physiol (2016): H1439
The study of the mechanism of arsenite toxicity in respiration-deficient cells reveals that NADPH oxidase-derived superoxide promotes the same downstream events mediated by mitochondrial superoxide in respiration-proficient cells
Authors: Guidarelli A, Fiorani M, Carloni S, Cerioni L, Balduini W, Cantoni O.
Journal: Toxicol Appl Pharmacol (2016): 35
Authors: Guidarelli A, Fiorani M, Carloni S, Cerioni L, Balduini W, Cantoni O.
Journal: Toxicol Appl Pharmacol (2016): 35
20-HETE-induced mitochondrial superoxide production and inflammatory phenotype in vascular smooth muscle is prevented by glucose-6-phosphate dehydrogenase inhibition
Authors: Lakhkar A, Dhagia V, Joshi SR, Gotlinger K, Patel D, Sun D, Wolin MS, Schwartzman ML, Gupte SA.
Journal: Am J Physiol Heart Circ Physiol (2016): H1107
Authors: Lakhkar A, Dhagia V, Joshi SR, Gotlinger K, Patel D, Sun D, Wolin MS, Schwartzman ML, Gupte SA.
Journal: Am J Physiol Heart Circ Physiol (2016): H1107