DiTO™-1 [equivalent to TOTO®-1] *5 mM DMSO Solution* *CAS 143413-84-7*

DiTO™-1

equivalent to TOTO®-1; 5 mM DMSO Solution; CAS 143413-84-7

DiTO™-1 is chemically equivalent to TOTO®-1 (TOTO® is the trademark of Invitrogen). DiTO™-1 is a carbocyanine dimer with green fluorescence similar to FITC. It is cell-impermeant and easily distinguished from Cy5 and rhodamines as a nuclear counterstain and dead cell indicator. It is non-fluorescent in the absence of nucleic acids, and generates a very bright fluorescence signal upon binding to DNA. DiTO™-1 gives strong and selective nuclear staining in cultured cells and in paraffin sections. Simultaneous labeling with cell-permeable Nuclear Red™ LCS1 dye and cell-impermeant TOTO®-1 can be used to assess cell viability. DiTO™-1 and Nuclear Red™ both have much greater extinction coefficients than that of DNA-bound propidium iodide.

Protocol

SDS

Catalog	Size	Price	Quantity
17575	0.2 ml	Price

Citations

View all 4 citations: Citation Explorer

Biofilm formation on the surface of monazite and xenotime during bioleaching

Authors:

van Alin, Arya and Corbett, Melissa K and Fathollahzadeh, Homayoun and Tjiam, M Christian and Rickard, William DA and Sun, Xiao and Putnis, Andrew and Eksteen, Jacques and Kaksonen, Anna H and Watkin, Elizabeth

Journal:

Microbial Biotechnology (2023)

Highly efficient incorporation of dATP in terminal transferase polymerization forming the ploy (A) n-DITO-1 fluorescent probe sensing terminal transferase and T4 polynucleotide kinase activity

Authors:

Zhu, Jing and Chen, Liutao

Journal:

Analytica Chimica Acta (2022): 340080

Francisella novicida and F. philomiragia biofilm features conditionning fitness in spring water and in presence of antibiotics

Authors:

Siebert, Claire and Villers, Corinne and Pavlou, Georgios and Touquet, Bastien and Yakandawala, Nandadeva and Tardieux, Isabelle and Renesto, Patricia

Journal:

PloS one (2020): e0228591

Francisella novicida and F. philomiragia biofilm features conditionning fitness in spring water and in presence of antibiotics

Authors:

Siebert, Claire and Villers, Corinne and Pavlou, Georgios and Touquet, Bastien and Yakandawala, Nandadeva and Tardieux, Isabelle and Renesto, Patricia

Journal:

Plos one (2020): e0228591

References

View all 27 references: Citation Explorer

Chromatin staining of Drosophila testes

Authors:

Bonaccorsi S, Giansanti MG, Cenci G, Gatti M.

Journal:

Cold Spring Harb Protoc (2012)

In vivo cancer imaging by poly(ethylene glycol)-b-poly(varepsilon-caprolactone) micelles containing a near-infrared probe

Authors:

Cho H, Indig GL, Weichert J, Shin HC, Kwon GS.

Journal:

Nanomedicine (2012): 228

The fluorescent dyes TO-PRO-3 and TOTO-3 iodide allow detection of microbial cells in soil samples without interference from background fluorescence

Authors:

Henneberger R, Birch D, Bergquist P, Walter M, Anitori RP.

Journal:

Biotechniques (2011): 190

Effect of Mg ions on efficiency of gene electrotransfer and on cell electropermeabilization

Authors:

Haberl S, Miklavcic D, Pavlin M.

Journal:

Bioelectrochemistry (2010): 265

Visualizing nuclei in skin cryosections: viable options to 4'6-diamidino-2-phenylindol for confocal laser microscopy

Authors:

Glaser K, Wilke K, Wepf R, Biel SS.

Journal:

Skin Res Technol (2008): 324

Physical properties

Molecular weight	1302.78
Solvent	Water

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	117000 ¹
Excitation (nm)	514
Emission (nm)	531
Quantum yield	0.34 ¹

Storage, safety and handling

H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134
CAS	143413-84-7

Instrument settings

Fluorescence microscope
Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom
Instrument specification(s)	FITC filter set

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Cell Viability & Proliferation
Brochure: Nucleic Acid Detection Probes & Assay Kits

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Page updated on October 30, 2025