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Pluronic® F-127
10% solution in water
Pluronic® F127 is a detergent that can improve the water solubility of hydrophobic molecules, such as AM esters
and lipophilic tracers.
Pluronic® F-127, also known as poloxamer 407, is a surfactant polyol (polyoxypropylene-polyoxyethylene block
copolymer). It has a molecular weight of approximately 13 kDa and is provided as a 10% solution in water.
Given its amphiphilic nature, Pluronic® F127 has been found to help solubilize and disperse dye molecules in
physiological media, such as Hanks' Balanced Salt Solution (HBSS). Data shows that it is relatively non-toxic to
cells. [1, 2]
While concentration is often experimentally dependent, this table provides a guideline for some common Pluronic®
F-127 uses:
| Compound | Pluronic® F127 Concentration |
| Calcein AM | 0.02% |
| CFDA AM | 0.05% |
| Fluo-3 AM | 0.04% |
| Fluo-4 AM | 0.04% |
| Fura-2 AM | 0.04% |
| Rhod-2 AM | 0.04% |
- Hamad, M. I., Krause, M., & Wahle, P. (2015). Improving AM ester calcium dye loading efficiency. Journal of neuroscience methods, 240, 48-60.
- Poenie, M., Alderton, J., Steinhardt, R., & Tsien, R. (1986). Calcium rises abruptly and briefly throughout the cell at the onset of anaphase. Science, 233(4766), 886-889.
Citations
View all 25 citations: Citation Explorer
Mitochondrial-dependent oxidative phosphorylation is key for postnatal metabolic adaptation of alveolar macrophages in the lung
Authors: Zhang, Jun and Peng, Yu and Song, Haosen and Liu, Siqi and Li, Chuanwei and Zhang, Yi and Shi, Xiaowei and Guo, Huifang and Xu, Yingping
Journal: International Immunopharmacology (2024): 112012
Authors: Zhang, Jun and Peng, Yu and Song, Haosen and Liu, Siqi and Li, Chuanwei and Zhang, Yi and Shi, Xiaowei and Guo, Huifang and Xu, Yingping
Journal: International Immunopharmacology (2024): 112012
Agonist-Induced Ca2+ Signaling in HEK-293-Derived Cells Expressing a Single IP3 Receptor Isoform
Authors: Kochkina, Ekaterina N and Kopylova, Elizaveta Е and Rogachevskaja, Olga A and Kovalenko, Nina P and Kabanova, Natalia V and Kotova, Polina D and Bystrova, Marina F and Kolesnikov, Stanislav S
Journal: Cells (2024): 562
Authors: Kochkina, Ekaterina N and Kopylova, Elizaveta Е and Rogachevskaja, Olga A and Kovalenko, Nina P and Kabanova, Natalia V and Kotova, Polina D and Bystrova, Marina F and Kolesnikov, Stanislav S
Journal: Cells (2024): 562
FTO-mediated m6A demethylation regulates GnRH expression in the hypothalamus via the PLC$\beta$3/Ca2+/CAMK signalling pathway
Authors: Zang, Shaolian and Yin, Xiaoqin and Li, Pin
Journal: Communications Biology (2023): 1297
Authors: Zang, Shaolian and Yin, Xiaoqin and Li, Pin
Journal: Communications Biology (2023): 1297
CVD-associated SNPs with regulatory potential reveal novel non-coding disease genes
Authors: Zhu, Chaonan and Baumgarten, Nina and Wu, Meiqian and Wang, Yue and Das, Arka Provo and Kaur, Jaskiran and Ardakani, Fatemeh Behjati and Duong, Thanh Thuy and Pham, Minh Duc and Duda, Maria and others,
Journal: Human Genomics (2023): 1--17
Authors: Zhu, Chaonan and Baumgarten, Nina and Wu, Meiqian and Wang, Yue and Das, Arka Provo and Kaur, Jaskiran and Ardakani, Fatemeh Behjati and Duong, Thanh Thuy and Pham, Minh Duc and Duda, Maria and others,
Journal: Human Genomics (2023): 1--17
Analysis of Fatty Acid Metabolism in Fetal and Failing Hearts by Single-Cell RNA Sequencing Revealed SLC27A6 as a Critical Gene in Heart Maturation
Authors: Zhu, Wenjia and Zheng, Yufan and Liu, Jiaying and Zhao, Chao and Sun, Ning and Qu, Xiuxia and Yang, Hui
Journal: Acta Cardiologica Sinica (2023): 580
Authors: Zhu, Wenjia and Zheng, Yufan and Liu, Jiaying and Zhao, Chao and Sun, Ning and Qu, Xiuxia and Yang, Hui
Journal: Acta Cardiologica Sinica (2023): 580
Page updated on September 18, 2024
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