PE/Cy5 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*
AAT Bioquest's anti-human secondary antibodies have well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. This PE/Cy5-labeled secondary antibody was prepared using AAT Bioquest's proprietary labeling technology. It demonstrated much brighter signal compared to the similar PE/Cy5 goat anti-human IgG antibodies from other commercial sources, and thus can significantly increase assay sensitivities. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. This antibody was purified through affinity chromatography and conjugated to PE/Cy5 (ex/em = 565/666 nm). It is compatible with the 561 nm laser and 697/58 nm bandpass filter (for example, as in the BD FACSMelody™).
Spectrum
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Conjugation
We provide custom conjugation services for this antibody (eg. labeling of IgG antibody with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
Proteins | HRP, Alkaline Phosphatase, Streptavidin |
Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
Small Molecules | Biotin |
Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Page updated on November 21, 2024