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N6-Methyladenosine-Biotin conjugate
N6-Methyladenosine is an abundant modification in mRNA and is found within some viruses, and most eukaryotes including mammals, insects, plants and yeast. It is also found in tRNA, rRNA, and small nuclear RNA (snRNA) as well as several long non-coding RNA, such as Xist. The methylation of adenosine is directed by a large N6-Methyladenosine methyltransferase complex containing METTL3 as the SAM-binding sub-unit. The biological functions of m6A are mediated through a group of RNA binding proteins that specifically recognize the methylated adenosine on RNA. This biotinylated N6-Methyladenosine derivative can be used for detecting the RNA modifications related to N6-Methyladenosine.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of N6-Methyladenosine-Biotin conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 132.649 µL | 663.244 µL | 1.326 mL | 6.632 mL | 13.265 mL |
| 5 mM | 26.53 µL | 132.649 µL | 265.298 µL | 1.326 mL | 2.653 mL |
| 10 mM | 13.265 µL | 66.324 µL | 132.649 µL | 663.244 µL | 1.326 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Citations
View all 28 citations: Citation Explorer
HIV-1 envelope proteins up-regulate N (6)-methyladenosine levels of cellular RNA independently of viral replication
Authors: Tirumuru, N., Wu, L.
Journal: J Biol Chem (2019): 3249-3260
Authors: Tirumuru, N., Wu, L.
Journal: J Biol Chem (2019): 3249-3260
Changes of RNA N(6)-methyladenosine in the hormesis effect induced by arsenite on human keratinocyte cells
Authors: Chen, H., Zhao, T., Sun, D., Wu, M., Zhang, Z.
Journal: Toxicol In Vitro (2019): 84-92
Authors: Chen, H., Zhao, T., Sun, D., Wu, M., Zhang, Z.
Journal: Toxicol In Vitro (2019): 84-92
N6-methyladenosine demethylase FTO suppresses clear cell renal cell carcinoma through a novel FTO-PGC-1alpha signalling axis
Authors: Zhuang, C., Zhuang, C., Luo, X., Huang, X., Yao, L., Li, J., Li, Y., Xiong, T., Ye, J., Zhang, F., Gui, Y.
Journal: J Cell Mol Med (2019): 2163-2173
Authors: Zhuang, C., Zhuang, C., Luo, X., Huang, X., Yao, L., Li, J., Li, Y., Xiong, T., Ye, J., Zhang, F., Gui, Y.
Journal: J Cell Mol Med (2019): 2163-2173
A dynamic reversible RNA N(6) -methyladenosine modification: current status and perspectives
Authors: Bi, Z., Liu, Y., Zhao, Y., Yao, Y., Wu, R., Liu, Q., Wang, Y., Wang, X.
Journal: J Cell Physiol (2019): 7948-7956
Authors: Bi, Z., Liu, Y., Zhao, Y., Yao, Y., Wu, R., Liu, Q., Wang, Y., Wang, X.
Journal: J Cell Physiol (2019): 7948-7956
Transcriptome-wide analysis of N6-methyladenosine uncovers its regulatory role in gene expression in the lepidopteran Bombyx mori
Authors: Li, B., Wang, X., Li, Z., Lu, C., Zhang, Q., Chang, L., Li, W., Cheng, T., Xia, Q., Zhao, P.
Journal: Insect Mol Biol (2019): se name="17100.enl" path="C:\Users\aatbi\Drop
Authors: Li, B., Wang, X., Li, Z., Lu, C., Zhang, Q., Chang, L., Li, W., Cheng, T., Xia, Q., Zhao, P.
Journal: Insect Mol Biol (2019): se name="17100.enl" path="C:\Users\aatbi\Drop
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