MitoROS Brite™ 670 *Optimized for Detecting Reactive Oxygen Species (ROS) in Mitochondria*
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Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Molecular weight | 758.87 |
Solvent | DMSO |
Excitation (nm) | 651 |
Emission (nm) | 670 |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Overview | ![]() ![]() |
Molecular weight 758.87 | Excitation (nm) 651 | Emission (nm) 670 |
Platform
Fluorescence microscope
Excitation | Cy5 Filter Set |
Emission | Cy5 Filter Set |
Recommended plate | Black wall/clear bottom |
Example protocol
AT A GLANCE
Before use, thaw MitoROS Brite™ 670 at room temperature. Once thawed, briefly centrifuge to collect the dried pellet.
Prepare the cells in a growth medium.
Stain the cells using the MitoROS Brite™ 670 working solution.
Treat the cells with your desired test compounds.
Monitor fluorescence intensity with a Cy5 filter set or Ex/Em = 650/670 nm.
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles
Prepare a 5 to 10 mM MitoROS Brite™ 670 stock solution in DMSO.
Note: Prepare single-use aliquots of the MitoROS Brite™ 670 stock solution and store at ≤ -20°C, protected from light. Avoid freeze-thaw cycles.
PREPARATION OF WORKING SOLUTION
Prepare a 5 to 10 μM MitoROS Brite™ 670 working solution by diluting the MitoROS Brite™ 670 stock solution into Hanks solution with 20 mM Hepes buffer (HHBS).
Note: For optimal results, use this solution within a few hours of preparation.
Note: Protect the working solution from light by covering it with foil or storing it in a dark place.
SAMPLE EXPERIMENTAL PROTOCOL
Plate the cells as desired in a 96-well black wall-clear bottom plate.
Add 100 µL of the MitoROS Brite™ 670 working solution to the cells.
Incubate the cells at 37°C for 30 to 60 minutes, protected from light.
Note: The optimal concentration and incubation time for MitoROS Brite™ 670 may vary between different cell lines. You may need to test different concentrations to determine the best conditions.
Remove the dye working solution and wash the cells twice with HHBS buffer.
Treat cells as desired.
Remove the treatment and wash cells twice with HHBS buffer.
Add HHBS buffer and examine the cells using a fluorescence microscope with a Cy5 filter set.
Calculators
Common stock solution preparation
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 131.775 µL | 658.874 µL | 1.318 mL | 6.589 mL | 13.177 mL |
5 mM | 26.355 µL | 131.775 µL | 263.55 µL | 1.318 mL | 2.635 mL |
10 mM | 13.177 µL | 65.887 µL | 131.775 µL | 658.874 µL | 1.318 mL |
Molarity calculator
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Product Family
Name | Excitation (nm) | Emission (nm) |
MitoROS Brite™ 570 *Optimized for Detecting Reactive Oxygen Species (ROS) in Mitochondria* | 555 | 568 |
ROS Brite™ 670 *Optimized for Detecting Reactive Oxygen Species (ROS)* | 651 | 670 |
Images
References
Authors: Chen, Wenhao and Shen, Zile and Dong, Wenxi and Huang, Guowei and Yu, Dingye and Chen, Weizhe and Yan, Xialin and Yu, Zhen
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Journal: Redox report : communications in free radical research (2024): 2312320
Authors: Liu, Xue and Wang, Fei-Yu and Chi, Song and Liu, Tao and Yang, Hai-Lin and Zhong, Ru-Jie and Li, Xiao-Yu and Gao, Jing
Journal: Brain research (2024): 148882
Authors: Vilas-Boas, Eloisa A and Kowaltowski, Alicia J
Journal: Free radical biology & medicine (2024): 195-214
Authors: Yao, Hanlin and Zhao, Hongchao and Du, Yang and Zhang, Ye and Li, Yanze and Zhu, Hengcheng
Journal: Translational research : the journal of laboratory and clinical medicine (2024): 1-12
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Journal: Redox biology (2024): 103174
Authors: Mialet-Perez, Jeanne and Belaidi, Elise
Journal: Free radical biology & medicine (2024): 13-22
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Journal: Biomaterials advances (2024): 213858
Authors: Xu, Zehua and Li, Jing and Su, Bowen and Gao, Hongying and Ren, Miaomiao and Lin, Yi and Shen, Heqing
Journal: Free radical biology & medicine (2024): 249-261
Authors: Milan, Madison and Brown, Jacob and O'Reilly, Colleen L and Bubak, Matthew P and Negri, Sharon and Balasubramanian, Priya and Dhanekula, Arjune S and Pharaoh, Gavin and Reyff, Zeke and Ballard, Cade and Shi, Helen and Yabluchanskiy, Andriy and Rudolph, Michael C and Ungvari, Zoltan and Marcinek, David J and Miller, Benjamin F and Van Remmen, Holly and Tarantini, Stefano
Journal: Redox biology (2024): 103189