MitoROS Brite™ 570 *Optimized for Detecting Reactive Oxygen Species (ROS) in Mitochondria*
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Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Molecular weight | 732.83 |
Solvent | DMSO |
Excitation (nm) | 555 |
Emission (nm) | 568 |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Overview | ![]() ![]() |
Molecular weight 732.83 | Excitation (nm) 555 | Emission (nm) 568 |
Platform
Fluorescence microscope
Excitation | Cy3 Filter Set |
Emission | Cy3 Filter Set |
Recommended plate | Black wall/clear bottom |
Example protocol
AT A GLANCE
Before use, thaw MitoROS Brite™ 570 at room temperature. Once thawed, briefly centrifuge to collect the dried pellet.
Prepare the cells in a growth medium.
Stain the cells using the MitoROS Brite™ 570 working solution.
Treat the cells with your desired test compounds.
Monitor fluorescence intensity with a Cy3 filter set or Ex/Em = 540/590 nm.
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles
Prepare a 5 to 10 mM MitoROS Brite™ 570 stock solution in DMSO.
Note: Prepare single-use aliquots of the MitoROS Brite™ 570 stock solution and store at ≤ -20°C, protected from light. Avoid freeze-thaw cycles.
PREPARATION OF WORKING SOLUTION
Prepare a 5 to 10 μM MitoROS Brite™ 570 working solution by diluting the MitoROS Brite™ 570 stock solution into Hanks solution with 20 mM Hepes buffer (HHBS).
Note: For optimal results, use this solution within a few hours of preparation.
Note: Protect the working solution from light by covering it with foil or storing it in a dark place.
SAMPLE EXPERIMENTAL PROTOCOL
Plate the cells as desired in a 96-well black wall-clear bottom plate.
Add 100 µL of the MitoROS Brite™ 570 working solution to the cells.
Incubate the cells at 37°C for 30 to 60 minutes, protected from light.
Note: The optimal concentration and incubation time for MitoROS Brite™ 570 may vary between different cell lines. You may need to test different concentrations to determine the best conditions.
Remove the dye working solution and wash the cells twice with HHBS buffer.
Treat cells as desired.
Remove the treatment and wash cells twice with HHBS buffer.
Add HHBS buffer and examine the cells using a fluorescence microscope with a Cy3 filter set.
Calculators
Common stock solution preparation
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 136.457 µL | 682.286 µL | 1.365 mL | 6.823 mL | 13.646 mL |
5 mM | 27.291 µL | 136.457 µL | 272.915 µL | 1.365 mL | 2.729 mL |
10 mM | 13.646 µL | 68.229 µL | 136.457 µL | 682.286 µL | 1.365 mL |
Molarity calculator
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Product Family
Name | Excitation (nm) | Emission (nm) |
MitoROS Brite™ 670 *Optimized for Detecting Reactive Oxygen Species (ROS) in Mitochondria* | 651 | 670 |
ROS Brite™ 570 *Optimized for Detecting Reactive Oxygen Species (ROS)* | 555 | 568 |
Images
References
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