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MitoLite™ Blue FX490
MitoLite™ Blue is a cationic dye that selectively accumulates in mitochondria probably via the mitochondrial membrane potential gradient. The mitochondrial indicator is a hydrophobic compound that easily permeates intact live cells, and trapped in mitochondria after it gets into cells. This fluorescent mitochondrial indicator is retained in mitochondria for long time since the indicator carries a cell-retaining group. This key feature significantly increases its staining efficiency. The labeling protocol is robust, requiring minimal hands-on time. It can be readily adapted for a wide variety of fluorescence platforms such as microplate assays, immunocytochemistry and flow cytometry. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.
Spectrum
Citations
View all 8 citations: Citation Explorer
Mitofusin-mediated contacts between mitochondria and peroxisomes regulate mitochondrial fusion
Authors: Alsayyah, Cynthia and Singh, Manish K and Morcillo-Parra, Maria Angeles and Cavellini, Laetitia and Shai, Nadav and Schmitt, Christine and Schuldiner, Maya and Zalckvar, Einat and Mallet, Adeline and Belgareh-Touz{\'e}, Na{\"\i}ma and others,
Journal: PLoS biology (2024): e3002602
Authors: Alsayyah, Cynthia and Singh, Manish K and Morcillo-Parra, Maria Angeles and Cavellini, Laetitia and Shai, Nadav and Schmitt, Christine and Schuldiner, Maya and Zalckvar, Einat and Mallet, Adeline and Belgareh-Touz{\'e}, Na{\"\i}ma and others,
Journal: PLoS biology (2024): e3002602
A selective fluorescent turn-on probe for imaging and sensing of hydrogen peroxide in living cells
Authors: Ciou, Jyun-Jia and Wu, Tzu-Chien and Chen, Zhi-Jia and Cheng, Bill and Lai, Chian-Hui
Journal: Analytical and Bioanalytical Chemistry (2023): 1--11
Authors: Ciou, Jyun-Jia and Wu, Tzu-Chien and Chen, Zhi-Jia and Cheng, Bill and Lai, Chian-Hui
Journal: Analytical and Bioanalytical Chemistry (2023): 1--11
Cetyltrimethylammonium chloride-loaded mesoporous silica nanoparticles as a mitochondrion-targeting agent for tumor therapy
Authors: Tang, Menghuan and Zhang, Peng and Liu, Jiahui and Long, Yijuan and Cheng, Yuan and Zheng, Huzhi
Journal: RSC Advances (2020): 17050--17057
Authors: Tang, Menghuan and Zhang, Peng and Liu, Jiahui and Long, Yijuan and Cheng, Yuan and Zheng, Huzhi
Journal: RSC Advances (2020): 17050--17057
A ratiometric fluorescent probe for detecting hypochlorite in the endoplasmic reticulum
Authors: Hou, Ji-Ting and Kim, Hyeong Seok and Duan, Chong and Ji, Myung Sun and Wang, Shan and Zeng, Lintao and Ren, Wen Xiu and Kim, Jong Seung
Journal: Chemical communications (2019): 2533--2536
Authors: Hou, Ji-Ting and Kim, Hyeong Seok and Duan, Chong and Ji, Myung Sun and Wang, Shan and Zeng, Lintao and Ren, Wen Xiu and Kim, Jong Seung
Journal: Chemical communications (2019): 2533--2536
Co-delivery of VP-16 and Bcl-2-targeted antisense on PEG-grafted oMWCNTs for synergistic in vitro anti-cancer effects in non-small and small cell lung cancer
Authors: Heger, Zbynek and Polanska, Hana and Krizkova, Sona and Balvan, Jan and Raudenska, Martina and Dostalova, Simona and Moulick, Amitava and Masarik, Michal and Adam, Vojtech
Journal: Colloids and Surfaces B: Biointerfaces (2017): 131--140
Authors: Heger, Zbynek and Polanska, Hana and Krizkova, Sona and Balvan, Jan and Raudenska, Martina and Dostalova, Simona and Moulick, Amitava and Masarik, Michal and Adam, Vojtech
Journal: Colloids and Surfaces B: Biointerfaces (2017): 131--140
References
View all 70 references: Citation Explorer
Quantification of carbonylated proteins in rat skeletal muscle mitochondria using capillary sieving electrophoresis with laser-induced fluorescence detection
Authors: Feng J, Arriaga EA.
Journal: Electrophoresis (2008): 475
Authors: Feng J, Arriaga EA.
Journal: Electrophoresis (2008): 475
Calcium, mitochondria and apoptosis studied by fluorescence measurements
Authors: Roy SS, Hajnoczky G.
Journal: Methods (2008): 213
Authors: Roy SS, Hajnoczky G.
Journal: Methods (2008): 213
Fluorescence imaging of mitochondria in yeast
Authors: Swayne TC, Gay AC, Pon LA.
Journal: Methods Mol Biol (2007): 433
Authors: Swayne TC, Gay AC, Pon LA.
Journal: Methods Mol Biol (2007): 433
A fluorescence assay for peptide translocation into mitochondria
Authors: Martinez-Caballero S, Peixoto PM, Kinnally KW, Campo ML.
Journal: Anal Biochem (2007): 76
Authors: Martinez-Caballero S, Peixoto PM, Kinnally KW, Campo ML.
Journal: Anal Biochem (2007): 76
Fast electrophoretic analysis of individual mitochondria using microchip capillary electrophoresis with laser induced fluorescence detection
Authors: Duffy CF, MacCraith B, Diamond D, O'Kennedy R, Arriaga EA.
Journal: Lab Chip (2006): 1007
Authors: Duffy CF, MacCraith B, Diamond D, O'Kennedy R, Arriaga EA.
Journal: Lab Chip (2006): 1007
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