mFluor™ UV520 maleimide
mFluor™ UV520 Maleimide is an excellent building block that can be readily used for labeling biomolecules that have a free thiol (SH) group such as antibodies and thiol-modified oligos. mFluor™ UV 520 dyes have fluorescence excitation and emission maxima of ~350 nm and ~520 nm respectively. These spectral characteristics make them a unique color for flow cytometry application. mFluor™ UV520 Maleimide is reasonably stable and shows good reactivity and selectivity with protein amino groups. mFluor™ UV520 Maleimide provides a convenient tool to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) for flow cytometric applications with the UV laser excitation. mFluor™ UV dyes are optimized to be excited with a UV laser at 350 nm. AAT Bioquest offers the largest collection of fluorescent dyes that are excited by UV laser at 350 nm. mFluor™ dyes are developed for multicolor flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 350 nm, 405 nm, 488 nm and 633 nm).
Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
mFluor™ UV520 SE | 503 | 524 | 800001 | - | 0.495 | 0.518 |
mFluor™ UV420 maleimide | 353 | 421 | 750001 | - | - | - |
mFluor™ UV540 maleimide | 542 | 560 | 900001 | 0.351 | 0.634 | 0.463 |
mFluor™ UV610 maleimide | 590 | 609 | 900001 | 0.25 | 0.949 | 0.904 |
mFluor™ UV460 maleimide | 358 | 456 | 150001 | 0.861 | 0.35 | 0.134 |