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AAT Bioquest

mFluor™ UV520 maleimide

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mFluor™ UV520 Maleimide is an excellent building block that can be readily used for labeling biomolecules that have a free thiol (SH) group such as antibodies and thiol-modified oligos. mFluor™ UV 520 dyes have fluorescence excitation and emission maxima of ~350 nm and ~520 nm respectively. These spectral characteristics make them a unique color for flow cytometry application. mFluor™ UV520 Maleimide is reasonably stable and shows good reactivity and selectivity with protein amino groups. mFluor™ UV520 Maleimide provides a convenient tool to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) for flow cytometric applications with the UV laser excitation. mFluor™ UV dyes are optimized to be excited with a UV laser at 350 nm. AAT Bioquest offers the largest collection of fluorescent dyes that are excited by UV laser at 350 nm. mFluor™ dyes are developed for multicolor flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 350 nm, 405 nm, 488 nm and 633 nm).
Ordering information
Price
Unit size
Catalog Number1607
Quantity
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Additional ordering information
Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
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Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
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Physical properties
Molecular weight1266.15
SolventDMSO
Spectral properties
Absorbance (nm)502
Correction Factor (260 nm)0.495
Correction Factor (280 nm)0.518
Extinction coefficient (cm -1 M -1)800001
Excitation (nm)503
Emission (nm)524
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
Spectrum
Product family
NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
mFluor™ UV520 SE503524800001-0.4950.518
mFluor™ UV420 maleimide353421750001---
mFluor™ UV540 maleimide5425609000010.3510.6340.463
mFluor™ UV610 maleimide5906099000010.250.9490.904
mFluor™ UV460 maleimide3584561500010.8610.350.134