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AAT Bioquest

Helixyte™ Green *20X Aqueous PCR Solution*

Helixyte™ Green is manufactured by AAT Bioquest, and it has the same chemical structure of SYBR® Green (SYBR® is the trademark of ThermoFisher). Helixyte™ Green is an excellent nucleic acid sensor. The dye is essentially nonfluorescent, but becomes highly fluorescent upon binding to dsDNA. Helixyte™ Green is much less mutagenic and toxic than ethidium bromide. It has the same spectral properties to those of PicoGreen®, thus a great replacement to PicoGreen® (PicoGreen® is the trademark of Invitrogen). Helixyte™ Green is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA). It has much greater sensitivity for dsDNA, thus especially useful for assays where the presence of contaminating RNA or ssDNA might obscure results. Helixyte™ Green dye has excitation and emission spectra identical to those of PicoGreen®, making the dye readily compatible with instrument settings of PicoGreen® or SYBR® Green. This particular Helixyte™ Green formulation is optimized for quantitative real-time PCR (qPCR) applications.

Spectrum

Citations

View all 1 citations: Citation Explorer
Molecular Characterisation and Antibody Response to Bovine Respiratory Syncytial Virus in Vaccinated and Infected Cattle in Turkey
Authors: Aydin, Ozge and Yilmaz, Aysun and Turan, Nuri and Richt, Juergen A and Yilmaz, Huseyin
Journal: Pathogens (2024): 304

References

View all 22 references: Citation Explorer
Plasmalemma permeability and necrotic cell death phenotypes after intracerebral hemorrhage in mice
Authors: Zhu X, Tao L, Tejima-M and eville E, Qiu J, Park J, Garber K, Ericsson M, Lo EH, Whalen MJ.
Journal: Stroke (2012): 524
Intra-organ Biodistribution of Gold Nanoparticles Using Intrinsic Two-photon Induced Photoluminescence
Authors: Park J, Estrada A, Schwartz JA, Diagaradjane P, Krishnan S, Dunn AK, Tunnell JW.
Journal: Lasers Surg Med (2010): 630
Screening by imaging: scaling up single-DNA-molecule analysis with a novel parabolic VA-TIRF reflector and noise-reduction techniques
Authors: van 't Hoff M, Reuter M, Dryden DT, Oheim M.
Journal: Phys Chem Chem Phys (2009): 7713
Novel anatomic structures in the brain and spinal cord of rabbit that may belong to the Bonghan system of potential acupuncture meridians
Authors: Lee BC, Kim S, Soh KS.
Journal: J Acupunct Meridian Stud (2008): 29
Triplet fraction buildup effect of the DNA-YOYO complex studied with fluorescence correlation spectroscopy
Authors: Shimizu M, Sasaki S, Kinjo M.
Journal: Anal Biochem (2007): 87
Page updated on November 21, 2024

Ordering information

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Catalog Number17591
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Physical properties

Molecular weight

N/A

Solvent

DMSO

Spectral properties

Excitation (nm)

498

Emission (nm)

522

Storage, safety and handling

H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC41116134