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Helixyte Green (SYBR Green I)

20X Aqueous PCR Solution
SYBR® Green I (Thermo Fisher Scientific) and Helixyte™ Green are chemically equivalent, nucleic acid stains, with an excitation peak at 498 nm and an emission peak at 522 nm. The dye is useful for the detection of double-stranded DNA (dsDNA) in gel electrophoresis and quantitative PCR (qPCR) applications.

Staining mechanism and principle

Helixyte™ Green is an intercalating nucleic acid dye belonging to the cyanine dye family. It is fluorogenic, becoming highly fluorescent upon binding to the minor groove of dsDNA and, to a lesser extent, when bound to single stranded DNA (ssDNA) and RNA. It can be used to stain dsDNA in polyacrylamide and agarose gels in electrophoresis applications. It is also useful for monitoring qPCR, wherein fluorescence measured during the annealing or extension stage is proportional to the amount of PCR product. In these applications, often the dye is provided alongside the master mix.

Excitation / Emission

Helixyte Green and SYBR Green I have the same fluorescence spectrum, with an excitation peak at 498 nm and an emission peak at 522 nm. They are well excited by the 488 nm laser line and can be detected in the FITC channel.

Helixyte/SYBR Green versus Ethidium Bromide (EtBr)

Helixyte™ Green and SYBR Green stains are in principle safer and less mutagenic than EtBr because they are less cell permeable. Additionally, these dyes are experimentally more convenient due to having a green excitation wavelength, which is compatible with common lab instruments, such as the FITC channel on a fluorescence microscope or the 488 nm laser on a flow cytometer. This is in contrast to EtBr, which requires the more dangerous UV excitation wavelength.

SYBR Green I versus SYBR Green II

SYBR Green I has a strong affinity for dsDNA, whereas SYBR Green II has a strong affinity for RNA.

Spectrum

Alternative formats

NameConcentration
Helixyte™ Green *10,000X Aqueous PCR Solution*10,000x
Helixyte™ Green *20X Aqueous PCR Solution*20x

Product family

NameExcitation (nm)Emission (nm)
Helixyte™ 14 [Equivalent to SYBR 14] *5 mM*489520
Helixyte™ 14 [Equivalent to SYBR 14]489520
Indocyanine Green *CAS 3599-32-4*789813
Thiolite™ Green505524
CytoTell™ Green510525
LysoBrite™ Green501510
Q4ever™ Green *1250X DMSO Solution*503527
Droplite™ Green421521
FerroBrite™ Green453552

Citations

View all 1 citations: Citation Explorer
Molecular Characterisation and Antibody Response to Bovine Respiratory Syncytial Virus in Vaccinated and Infected Cattle in Turkey
Authors: Aydin, Ozge and Yilmaz, Aysun and Turan, Nuri and Richt, Juergen A and Yilmaz, Huseyin
Journal: Pathogens (2024): 304

References

View all 22 references: Citation Explorer
Plasmalemma permeability and necrotic cell death phenotypes after intracerebral hemorrhage in mice
Authors: Zhu X, Tao L, Tejima-M and eville E, Qiu J, Park J, Garber K, Ericsson M, Lo EH, Whalen MJ.
Journal: Stroke (2012): 524
Intra-organ Biodistribution of Gold Nanoparticles Using Intrinsic Two-photon Induced Photoluminescence
Authors: Park J, Estrada A, Schwartz JA, Diagaradjane P, Krishnan S, Dunn AK, Tunnell JW.
Journal: Lasers Surg Med (2010): 630
Screening by imaging: scaling up single-DNA-molecule analysis with a novel parabolic VA-TIRF reflector and noise-reduction techniques
Authors: van 't Hoff M, Reuter M, Dryden DT, Oheim M.
Journal: Phys Chem Chem Phys (2009): 7713
Novel anatomic structures in the brain and spinal cord of rabbit that may belong to the Bonghan system of potential acupuncture meridians
Authors: Lee BC, Kim S, Soh KS.
Journal: J Acupunct Meridian Stud (2008): 29
Triplet fraction buildup effect of the DNA-YOYO complex studied with fluorescence correlation spectroscopy
Authors: Shimizu M, Sasaki S, Kinjo M.
Journal: Anal Biochem (2007): 87
Page updated on February 12, 2025

Ordering information

Price
Concentration20x
10,000x
20x
Unit size
Catalog Number
1759117592
Quantity
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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Request quotation

Physical properties

Molecular weight

N/A

Solvent

DMSO

Spectral properties

Excitation (nm)

498

Emission (nm)

522

Storage, safety and handling

H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC41116134
SYBR Green spectrum. SYBR Green is a fluorescent compound with an excitation peak at 498 nm and an emission peak at 522 nm. Other spectra of interest include: Helixyte™ Green Nucleic Acid Gel Stain, Helixyte™ Green dsDNA, and Q4ever™ Green. Helixyte™ Green belongs to the following categories: Kinases, Gene Expression Analysis & Genotyping, Genome Editing & CRISPR, Coronavirus (SARS-CoV-2), Nucleic Acid Building Blocks, Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR), RNA Immunoprecipitation (RIP), and Single Cell RNA Sequencing (scRNA-seq).
SYBR Green spectrum. SYBR Green is a fluorescent compound with an excitation peak at 498 nm and an emission peak at 522 nm. Other spectra of interest include: Helixyte™ Green Nucleic Acid Gel Stain, Helixyte™ Green dsDNA, and Q4ever™ Green. Helixyte™ Green belongs to the following categories: Kinases, Gene Expression Analysis & Genotyping, Genome Editing & CRISPR, Coronavirus (SARS-CoV-2), Nucleic Acid Building Blocks, Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR), RNA Immunoprecipitation (RIP), and Single Cell RNA Sequencing (scRNA-seq).
SYBR Green spectrum. SYBR Green is a fluorescent compound with an excitation peak at 498 nm and an emission peak at 522 nm. Other spectra of interest include: Helixyte™ Green Nucleic Acid Gel Stain, Helixyte™ Green dsDNA, and Q4ever™ Green. Helixyte™ Green belongs to the following categories: Kinases, Gene Expression Analysis & Genotyping, Genome Editing & CRISPR, Coronavirus (SARS-CoV-2), Nucleic Acid Building Blocks, Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR), RNA Immunoprecipitation (RIP), and Single Cell RNA Sequencing (scRNA-seq).
Quantitative PCR results targeting GAPDH with an input of 100 ng&ndash;0.00001 ng cDNA was performed using Helixyte&trade; Green *20X Aqueous PCR Solution* (Cat No. 17591)&nbsp;and a Fast Advanced Master Mix on an Applied Biosystems&reg; 7500 FAST Real-Time PCR System.

Documents

pdfSafety data sheet
pdfProduct protocol
pdfCertificate of analysis
Kinases
Gene Expression Analysis & Genotyping
Genome Editing & CRISPR
Coronavirus (SARS-CoV-2)
Nucleic Acid Building Blocks
Polymerase Chain Reaction (PCR)
Real-Time PCR (qPCR)
Reverse Transcription PCR (RT-PCR)
RNA Immunoprecipitation (RIP)
Single Cell RNA Sequencing (scRNA-seq)

Alternative formats

Helixyte™ Green *10,000X Aqueous PCR Solution*
Helixyte™ Green Nucleic Acid Gel Stain *10,000X DMSO Solution*
Helixyte™ Gold Nucleic Acid Gel Stain *10,000X DMSO Solution*
Helixyte™ Green dsDNA Quantifying Reagent *200X DMSO Solution*