Helixyte™ Green *20X Aqueous PCR Solution*
Helixyte™ Green is manufactured by AAT Bioquest, and it has the same chemical structure of SYBR® Green (SYBR® is the trademark of ThermoFisher). Helixyte™ Green is an excellent nucleic acid sensor. The dye is essentially nonfluorescent, but becomes highly fluorescent upon binding to dsDNA. Helixyte™ Green is much less mutagenic and toxic than ethidium bromide. It has the same spectral properties to those of PicoGreen®, thus a great replacement to PicoGreen® (PicoGreen® is the trademark of Invitrogen). Helixyte™ Green is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA). It has much greater sensitivity for dsDNA, thus especially useful for assays where the presence of contaminating RNA or ssDNA might obscure results. Helixyte™ Green dye has excitation and emission spectra identical to those of PicoGreen®, making the dye readily compatible with instrument settings of PicoGreen® or SYBR® Green. This particular Helixyte™ Green formulation is optimized for quantitative real-time PCR (qPCR) applications.
Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) |
Helixyte™ 14 [Equivalent to SYBR 14] *5 mM* | 489 | 520 |
Helixyte™ 14 [Equivalent to SYBR 14] | 489 | 520 |
Indocyanine Green *CAS 3599-32-4* | 789 | 813 |
Thiolite™ Green | 505 | 524 |
CytoTell™ Green | 510 | 525 |
LysoBrite™ Green | 501 | 510 |
Q4ever™ Green *1250X DMSO Solution* | 503 | 527 |
Droplite™ Green | 421 | 521 |
FerroBrite™ Green | 453 | 552 |
Citations
View all 1 citations: Citation Explorer
Molecular Characterisation and Antibody Response to Bovine Respiratory Syncytial Virus in Vaccinated and Infected Cattle in Turkey
Authors: Aydin, Ozge and Yilmaz, Aysun and Turan, Nuri and Richt, Juergen A and Yilmaz, Huseyin
Journal: Pathogens (2024): 304
Authors: Aydin, Ozge and Yilmaz, Aysun and Turan, Nuri and Richt, Juergen A and Yilmaz, Huseyin
Journal: Pathogens (2024): 304
References
View all 22 references: Citation Explorer
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Journal: Stroke (2012): 524
Authors: Zhu X, Tao L, Tejima-M and eville E, Qiu J, Park J, Garber K, Ericsson M, Lo EH, Whalen MJ.
Journal: Stroke (2012): 524
Intra-organ Biodistribution of Gold Nanoparticles Using Intrinsic Two-photon Induced Photoluminescence
Authors: Park J, Estrada A, Schwartz JA, Diagaradjane P, Krishnan S, Dunn AK, Tunnell JW.
Journal: Lasers Surg Med (2010): 630
Authors: Park J, Estrada A, Schwartz JA, Diagaradjane P, Krishnan S, Dunn AK, Tunnell JW.
Journal: Lasers Surg Med (2010): 630
Screening by imaging: scaling up single-DNA-molecule analysis with a novel parabolic VA-TIRF reflector and noise-reduction techniques
Authors: van 't Hoff M, Reuter M, Dryden DT, Oheim M.
Journal: Phys Chem Chem Phys (2009): 7713
Authors: van 't Hoff M, Reuter M, Dryden DT, Oheim M.
Journal: Phys Chem Chem Phys (2009): 7713
Novel anatomic structures in the brain and spinal cord of rabbit that may belong to the Bonghan system of potential acupuncture meridians
Authors: Lee BC, Kim S, Soh KS.
Journal: J Acupunct Meridian Stud (2008): 29
Authors: Lee BC, Kim S, Soh KS.
Journal: J Acupunct Meridian Stud (2008): 29
Triplet fraction buildup effect of the DNA-YOYO complex studied with fluorescence correlation spectroscopy
Authors: Shimizu M, Sasaki S, Kinjo M.
Journal: Anal Biochem (2007): 87
Authors: Shimizu M, Sasaki S, Kinjo M.
Journal: Anal Biochem (2007): 87
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