Helixyte™ Gold Nucleic Acid Gel Stain *10,000X DMSO Solution*
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 749.48 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 496 |
Emission (nm) | 539 |
Storage, safety and handling
H-phrase | H303, H313, H340 |
Hazard symbol | T |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R68 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 41116134 |
Related products
Overview | ![]() ![]() |
See also: Cloning, DNA Fragmentation, DNA & RNA Markers and Ladders, Gel Electrophoresis, Loop-Mediated Isothermal Amplification (LAMP), Polymerase Chain Reaction (PCR), Reverse Transcription PCR (RT-PCR), RNA Purification & Analysis
Molecular weight 749.48 | Excitation (nm) 496 | Emission (nm) 539 |
Helixyte™ Gold is manufactured by AAT Bioquest, and it has the same chemical structure of SYBR® Gold (SYBR® is the trademark of ThermoFisher). Helixyte™ Gold is an excellent nucleic acid gel stain, and exhibits large fluorescence enhancement upon binding to nucleic acids. It has the same spectral properties to those of SYBR® Gold, thus a great replacement to SYBR® Gold (SYBR® Gold is the trademark of ThermoFisher). It is one of the most sensitive stains available for detecting DNA in agarose and polyacrylamide gels. Helixyte™ Gold has higher sensitivity for DNA than RNA, and is ideal for use with laser scanners with the same instrument settings of SYBR Gold. Helixyte™ Gold is much more sensitive than ethidium bromide for DNA in agarose gels, and it can detect as low as picogram dsDNA on gels.
Example protocol
AT A GLANCE
Spectral Properties of Helixyte™ Gold
Excitation/Emission: 495/540 nm
Important Helixyte™ Gold nucleic acid gel stain is significantly less mutagenic than ethidium bromide. However, we must caution that no data are available on the mutagenicity or toxicity of Helixyte™ Gold stain in humans. It should be treated as a potential mutagen and used with appropriate care due to the fact that this reagent binds to nucleic acids. The disposing of the stain shall be in compliance with local regulations.
We have found the greatest sensitivity is achieved by post-staining which also eliminates the possibility of dye interference with DNA migration. While the precast protocol is more convenient, some DNA samples may experience migration, it is highly recommended the gel running time does not exceed more than 2 hours. The following protocols are recommended. However, some comparisons might be needed to determine which one better meets your needs.
Excitation/Emission: 495/540 nm
Important Helixyte™ Gold nucleic acid gel stain is significantly less mutagenic than ethidium bromide. However, we must caution that no data are available on the mutagenicity or toxicity of Helixyte™ Gold stain in humans. It should be treated as a potential mutagen and used with appropriate care due to the fact that this reagent binds to nucleic acids. The disposing of the stain shall be in compliance with local regulations.
We have found the greatest sensitivity is achieved by post-staining which also eliminates the possibility of dye interference with DNA migration. While the precast protocol is more convenient, some DNA samples may experience migration, it is highly recommended the gel running time does not exceed more than 2 hours. The following protocols are recommended. However, some comparisons might be needed to determine which one better meets your needs.
PREPARATION OF WORKING SOLUTION
Helixyte™ Gold working solution (1X)
Make 1X Helixyte™ Gold working solution by diluting the 10,000X stock reagent into pH 7.5 - 8 buffer (e.g., TAE, TBE or TE, preferably pH 8.0). Note Staining solutions prepared in water are less stable than those prepared in buffer and must be used within 24 hours to ensure maximal staining sensitivity.
Note In addition, staining solutions prepared in buffers with pH below 7.5 or above 8.0 are less stable and show reduced staining efficacy.
SAMPLE EXPERIMENTAL PROTOCOL
Post-Staining Protocol
- Run gels based on your standard protocol.
- Place the gel in a suitable polypropylene container. Gently add a sufficient amount of the 1X Helixyte™ Gold working solution to submerge the gel.
Note Do not use a glass container, as it will adsorb much of the dye in the staining solution. - Agitate the gel gently at room temperature for ~30 minutes, protected from the light.
Note The staining solution can be stored in the dark (preferably refrigerated) for a week and reused up to 2 - 3 times. - Image the stained gel with a 254 nm transilluminator or a laser-based gel scanner using a long path green filter, such as a SYBR® filter or GelStar® filter.
Pre-Casting Protocol
- Prepare agarose gel solution using your standard protocol.
- Add 1X Helixyte™ Gold working solution to the gel and mix thoroughly.
- Run gels based on your standard protocol.
- Image the stained gel with a 254 nm transilluminator or a laser-based gel scanner using a long path green filter, such as a SYBR® filter or GelStar® filter.
DNA-Staining Before Electrophoresis
- Incubate DNA with a 1:1000 to 1:3000 dilution of the dye (in TE, TBE, or TAE) for at least 15 minutes prior to electrophoresis.
- Run gels based on your standard protocol.
- Image the stained gel with a 254 nm transilluminator, or a laser-based gel scanner using a long path green filter such as a SYBR® filter or GelStar® filter.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Helixyte™ Gold Nucleic Acid Gel Stain *10,000X DMSO Solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 133.426 µL | 667.129 µL | 1.334 mL | 6.671 mL | 13.343 mL |
5 mM | 26.685 µL | 133.426 µL | 266.852 µL | 1.334 mL | 2.669 mL |
10 mM | 13.343 µL | 66.713 µL | 133.426 µL | 667.129 µL | 1.334 mL |
Molarity calculator
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Product Family
Name | Excitation (nm) | Emission (nm) |
Helixyte™ Green Nucleic Acid Gel Stain *10,000X DMSO Solution* | 498 | 522 |
Images
References
View all 79 references: Citation Explorer
SYBR green real-time PCR for the detection of all enterovirus-A71 genogroups
Authors: Dubot-Peres A, Tan CY, de Chesse R, Sibounheuang B, Vongsouvath M, Phommasone K, Bessaud M, Gazin C, Thirion L, Phetsouvanh R, Newton PN, de Lamballerie X.
Journal: PLoS One (2014): e89963
Authors: Dubot-Peres A, Tan CY, de Chesse R, Sibounheuang B, Vongsouvath M, Phommasone K, Bessaud M, Gazin C, Thirion L, Phetsouvanh R, Newton PN, de Lamballerie X.
Journal: PLoS One (2014): e89963
Development of loop-mediated isothermal amplification and SYBR green real-time PCR methods for the detection of Citrus yellow mosaic badnavirus in citrus species
Authors: Anthony Johnson AM, Dasgupta I, Sai Gopal DV.
Journal: J Virol Methods (2014): 9
Authors: Anthony Johnson AM, Dasgupta I, Sai Gopal DV.
Journal: J Virol Methods (2014): 9
Development of both multiple PCR and real-time SYBR green PCR for the detection of Vibrio cholerae non-O1/O139 serogroups
Authors: Li F, Kan B, Wang D.
Journal: Zhonghua Liu Xing Bing Xue Za Zhi (2014): 66
Authors: Li F, Kan B, Wang D.
Journal: Zhonghua Liu Xing Bing Xue Za Zhi (2014): 66
Detection of Cardamom mosaic virus and Banana bract mosaic virus in cardamom using SYBR Green based reverse transcription-quantitative PCR
Authors: Siljo A, Bhat AI, Biju CN.
Journal: Virusdisease (2014): 137
Authors: Siljo A, Bhat AI, Biju CN.
Journal: Virusdisease (2014): 137
Comparison of SYBR Green and TaqMan methods in quantitative real-time polymerase chain reaction analysis of four adenosine receptor subtypes
Authors: Tajadini M, Panjehpour M, Javanmard SH.
Journal: Adv Biomed Res (2014): 85
Authors: Tajadini M, Panjehpour M, Javanmard SH.
Journal: Adv Biomed Res (2014): 85
Development of a High-resolution Melting Analysis Method Based on SYBR Green-I for rs7216389 Locus Genotyping in Asthmatic Child Patients
Authors: Vali Z, Raz A, Bokharaei H, Nabavi M, Bemanian MH, Yazdi MS, Djadid ND.
Journal: Avicenna J Med Biotechnol (2014): 72
Authors: Vali Z, Raz A, Bokharaei H, Nabavi M, Bemanian MH, Yazdi MS, Djadid ND.
Journal: Avicenna J Med Biotechnol (2014): 72
Primer evaluation and adaption for cost-efficient SYBR Green-based qPCR and its applicability for specific quantification of methanogens
Authors: Reitschuler C, Lins P, Illmer P.
Journal: World J Microbiol Biotechnol (2014): 293
Authors: Reitschuler C, Lins P, Illmer P.
Journal: World J Microbiol Biotechnol (2014): 293
Development and comparative evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of West Nile virus in human patients
Authors: Kumar JS, Saxena D, Parida M.
Journal: Mol Cell Probes. (2014)
Authors: Kumar JS, Saxena D, Parida M.
Journal: Mol Cell Probes. (2014)
Multiplex SYBR(R) green-real time PCR (qPCR) assay for the detection and differentiation of Bartonella henselae and Bartonella clarridgeiae in cats
Authors: Staggemeier R, Pilger DA, Spilki FR, Cantarelli VV.
Journal: Rev Inst Med Trop Sao Paulo (2014): 93
Authors: Staggemeier R, Pilger DA, Spilki FR, Cantarelli VV.
Journal: Rev Inst Med Trop Sao Paulo (2014): 93
Development and evaluation of SYBR Green-I based quantitative PCR assays for herpes simplex virus type 1 whole transcriptome analysis
Authors: Garvey CE, McGowin CL, Foster TP.
Journal: J Virol Methods (2014): 101
Authors: Garvey CE, McGowin CL, Foster TP.
Journal: J Virol Methods (2014): 101
Application notes
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