FMOC-Lys(Biotin)-OH

FMOC-Lys(Biotin)-OH is a lysine derivative for the preparation of biotin-labeled peptides by FMOC-SPPS. It is useful for preparing biotin-labeled peptides. The biotinylated peptides are widely used in biological detections in combination with enzyme-labeled or fluorescence-labeled avidin or streptavidin conjugates. Due to the multiple sites of avidin conjugates, the binding of labeled avidin to a biotinylated probe produces an amplified response compared to the direct incorporation of the label into the peptides.

Calculators

Common stock solution preparation

Table 1. Volume of DMF needed to reconstitute specific mass of FMOC-Lys(Biotin)-OH to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	168.144 µL	840.718 µL	1.681 mL	8.407 mL	16.814 mL
5 mM	33.629 µL	168.144 µL	336.287 µL	1.681 mL	3.363 mL
10 mM	16.814 µL	84.072 µL	168.144 µL	840.718 µL	1.681 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (280 nm)	Correction Factor (260 nm)
FMOC-Lys(DABCYL)-OH CAS 146998-27-8	-	-	-	-	0.516	-
FMOC-Lys(TF3)-OH	553	578	75000¹	0.1¹	0.179	-
FMOC-Lys(Cy3)-OH	555	569	150000¹	0.15¹	0.073	0.07
FMOC-Lys(Cy5)-OH	651	670	250000¹	0.27¹, 0.4²	0.03	0.02
FMOC-Lys(TQ2)-OH	-	-	21000	-	0.12	0.100
FMOC-Lys(TQ3)-OH	-	-	22000	-	0.091	0.085

Citations

View all 2 citations: Citation Explorer

Pharmacophore Generation from a Drug-like Core Molecule Surrounded by a Library Peptide via the 10BASEd-T on Bacteriophage T7
Authors: Tokunaga, Yuuki and Azetsu, Yuuki and Fukunaga, Keisuke and Hatanaka, Takaaki and Ito, Yuji and Taki, Masumi
Journal: Molecules (2014): 2481--2496

Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions
Authors: Guimaraes, Carla P and Witte, Martin D and Theile, Christopher S and Bozkurt, Gunes and Kundrat, Lenka and Blom, Annet EM and Ploegh, Hidde L
Journal: Nature protocols (2013): 1787--1799

References

View all 16 references: Citation Explorer

Profiling the substrate specificity of viral protease VP4 by a FRET-based peptide library approach
Authors: Ekici OD, Zhu J, Wah Chung IY, Paetzel M, Dalbey RE, Pei D.
Journal: Biochemistry (2009): 5753

A FRET-based assay for characterization of alternative splicing events using peptide nucleic acid fluorescence in situ hybridization
Authors: Blanco AM, Rausell L, Aguado B, Perez-Alonso M, Artero R.
Journal: Nucleic Acids Res (2009): e116

Unfolded protein and peptide dynamics investigated with single-molecule FRET and correlation spectroscopy from picoseconds to seconds
Authors: Nettels D, Hoffmann A, Schuler B.
Journal: J Phys Chem B (2008): 6137

Development of DNA aptamers to a foot-and-mouth disease peptide for competitive FRET-based detection
Authors: Bruno JG, Carrillo MP, Phillips T.
Journal: J Biomol Tech (2008): 109

Synthesis of peptide nucleic acid FRET probes via an orthogonally protected building block for post-synthetic labeling of peptide nucleic acids at the 5-position of uracil
Authors: Oquare BY, Taylor JS.
Journal: Bioconjug Chem (2008): 2196

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