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DY547 CEP [DyLight 547 phosphoramidite]
DY547 CEP (DyLight 547 phosphoramidite) is an excellent replacement to the commonly used Cy3 phosphoramidite. Two cyanine derivatives, Cy3 and Cy5 phosphoramidites, are the most popular fluorescent labeling reagents for preparing fluorescence-labeled oligonucleotides. However, both Cy3 and Cy5 phosphoramidites are not very stable. DY547 CEP appears to be more stable than Cy3 phosphoramidite. As Cy3 and Cy5 phosphoramidites, the deprotection of oligos containing DY547 CEP may be carried out with ammonium hydroxide at room temperature, regardless of the base protecting groups on the monomers used. However, it is always prudent to use monomers with base labile protecting groups to limit the exposure time to 2 hours or less at 65°C during deprotection.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| DY647 CEP [DyLight 647 phosphoramidite] | 652 | 673 | 2500001 |
| DY677 CEP [DyLight 677 phosphoramidite] | 682 | 702 | - |
References
View all 50 references: Citation Explorer
Probing functional conformation-state fluctuation dynamics in recognition binding between calmodulin and target peptide.
Authors: Jaiswal, Sunidhi and He, Yufan and Lu, H Peter
Journal: The Journal of chemical physics (2022): 055102
Authors: Jaiswal, Sunidhi and He, Yufan and Lu, H Peter
Journal: The Journal of chemical physics (2022): 055102
Insulin-Like Growth Factor-1 Receptor Targeted Fluorescent Imaging for Gallbladder Cancer in Orthotopic Mouse Models.
Authors: Choi, Jung Ha and Park, Jeong Youp
Journal: Gut and liver (2021)
Authors: Choi, Jung Ha and Park, Jeong Youp
Journal: Gut and liver (2021)
Stability Enhancement of a Dimeric HER2-Specific Affibody Molecule through Sortase A-Catalyzed Head-to-Tail Cyclization.
Authors: Westerlund, Kristina and Myrhammar, Anders and Tano, Hanna and Gestin, Maxime and Karlström, Amelie Eriksson
Journal: Molecules (Basel, Switzerland) (2021)
Authors: Westerlund, Kristina and Myrhammar, Anders and Tano, Hanna and Gestin, Maxime and Karlström, Amelie Eriksson
Journal: Molecules (Basel, Switzerland) (2021)
Synthesis of Fluorescently Labeled Antibodies Using Non-Canonical Amino Acids in Eukaryotic Cell-Free Systems.
Authors: Stech, Marlitt and Rakotoarinoro, Nathanaël and Teichmann, Tamara and Zemella, Anne and Thoring, Lena and Kubick, Stefan
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 175-190
Authors: Stech, Marlitt and Rakotoarinoro, Nathanaël and Teichmann, Tamara and Zemella, Anne and Thoring, Lena and Kubick, Stefan
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 175-190
Novel EBV LMP1 C-terminal domain binding affibody molecules as potential agents for in vivo molecular imaging diagnosis of nasopharyngeal carcinoma.
Authors: Kamara, Saidu and Guo, Yanru and Mao, Shanshan and Ye, Xiaoxian and Li, Qingfeng and Zheng, Maolin and Zhu, Jinshun and Zhang, Jing and Du, Wangqi and Chen, Jun and Zhu, Shanli and Zhang, Lifang
Journal: Applied microbiology and biotechnology (2021): 7283-7293
Authors: Kamara, Saidu and Guo, Yanru and Mao, Shanshan and Ye, Xiaoxian and Li, Qingfeng and Zheng, Maolin and Zhu, Jinshun and Zhang, Jing and Du, Wangqi and Chen, Jun and Zhu, Shanli and Zhang, Lifang
Journal: Applied microbiology and biotechnology (2021): 7283-7293
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