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DY647 CEP [DyLight 647 phosphoramidite]
DY647 CEP (DyLight 647 phosphoramidite) is an excellent replacement to the commonly used Cy5 phosphoramidite. Two cyanine derivatives, Cy3 and Cy5 phosphoramidites, are the most popular fluorescent labeling reagents for preparing fluorescence-labeled oligonucleotides. However, both Cy3 and Cy5 phosphoramidites are not very stable. DY647 CEP appears to be more stable than Cy5 phosphoramidite. As Cy3 and Cy5 phosphoramidites, the deprotection of oligos containing DY647 CEP may be carried out with ammonium hydroxide at room temperature, regardless of the base protecting groups on the monomers used. However, it is always prudent to use monomers with base labile protecting groups to limit the exposure time to 2 hours or less at 65°C during deprotection.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| DY677 CEP [DyLight 677 phosphoramidite] | 682 | 702 | - |
| DY547 CEP [DyLight 547 phosphoramidite] | 558 | 574 | 1500001 |
References
View all 3 references: Citation Explorer
Resonance energy transfer in DNA duplexes labeled with localized dyes.
Authors: Cunningham, Paul D and Khachatrian, Ani and Buckhout-White, Susan and Deschamps, Jeffrey R and Goldman, Ellen R and Medintz, Igor L and Melinger, Joseph S
Journal: The journal of physical chemistry. B (2014): 14555-65
Authors: Cunningham, Paul D and Khachatrian, Ani and Buckhout-White, Susan and Deschamps, Jeffrey R and Goldman, Ellen R and Medintz, Igor L and Melinger, Joseph S
Journal: The journal of physical chemistry. B (2014): 14555-65
A highly fluorescent DNA toolkit: synthesis and properties of oligonucleotides containing new Cy3, Cy5 and Cy3B monomers.
Authors: Hall, Lucy M and Gerowska, Marta and Brown, Tom
Journal: Nucleic acids research (2012): e108
Authors: Hall, Lucy M and Gerowska, Marta and Brown, Tom
Journal: Nucleic acids research (2012): e108
Hairpin-like fluorescent probe for imaging of NF-κB transcription factor activity.
Authors: Metelev, Valeri and Zhang, Surong and Tabatadze, David and Bogdanov, Alexei
Journal: Bioconjugate chemistry (2011): 759-65
Authors: Metelev, Valeri and Zhang, Surong and Tabatadze, David and Bogdanov, Alexei
Journal: Bioconjugate chemistry (2011): 759-65
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