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DY677 CEP [DyLight 677 phosphoramidite]
DY677 CEP (DyLight 677 phosphoramidite) is an excellent replacement to the commonly used Cy5.5 phosphoramidite. Cyanine phosphoramidites (such as Cy3 and Cy5 phosphoramidites) are the most popular fluorescent labeling reagents for preparing fluorescence-labeled oligonucleotides. However, both Cy3 and Cy5 phosphoramidites are not very stable. DY677 CEP appears to be more stable than Cy5.5 phosphoramidite. As Cy5.5 phosphoramidite, the deprotection of oligos containing DY677 CEP may be carried out with ammonium hydroxide at room temperature, regardless of the base protecting groups on the monomers used. However, it is always prudent to use monomers with base labile protecting groups to limit the exposure time to 2 hours or less at 65°C during deprotection.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| DY647 CEP [DyLight 647 phosphoramidite] | 652 | 673 | 2500001 |
| DY547 CEP [DyLight 547 phosphoramidite] | 558 | 574 | 1500001 |
References
View all 50 references: Citation Explorer
Insulin-Like Growth Factor-1 Receptor Targeted Fluorescent Imaging for Gallbladder Cancer in Orthotopic Mouse Models.
Authors: Choi, Jung Ha and Park, Jeong Youp
Journal: Gut and liver (2021)
Authors: Choi, Jung Ha and Park, Jeong Youp
Journal: Gut and liver (2021)
Stability Enhancement of a Dimeric HER2-Specific Affibody Molecule through Sortase A-Catalyzed Head-to-Tail Cyclization.
Authors: Westerlund, Kristina and Myrhammar, Anders and Tano, Hanna and Gestin, Maxime and Karlström, Amelie Eriksson
Journal: Molecules (Basel, Switzerland) (2021)
Authors: Westerlund, Kristina and Myrhammar, Anders and Tano, Hanna and Gestin, Maxime and Karlström, Amelie Eriksson
Journal: Molecules (Basel, Switzerland) (2021)
Synthesis of Fluorescently Labeled Antibodies Using Non-Canonical Amino Acids in Eukaryotic Cell-Free Systems.
Authors: Stech, Marlitt and Rakotoarinoro, Nathanaël and Teichmann, Tamara and Zemella, Anne and Thoring, Lena and Kubick, Stefan
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 175-190
Authors: Stech, Marlitt and Rakotoarinoro, Nathanaël and Teichmann, Tamara and Zemella, Anne and Thoring, Lena and Kubick, Stefan
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 175-190
Novel EBV LMP1 C-terminal domain binding affibody molecules as potential agents for in vivo molecular imaging diagnosis of nasopharyngeal carcinoma.
Authors: Kamara, Saidu and Guo, Yanru and Mao, Shanshan and Ye, Xiaoxian and Li, Qingfeng and Zheng, Maolin and Zhu, Jinshun and Zhang, Jing and Du, Wangqi and Chen, Jun and Zhu, Shanli and Zhang, Lifang
Journal: Applied microbiology and biotechnology (2021): 7283-7293
Authors: Kamara, Saidu and Guo, Yanru and Mao, Shanshan and Ye, Xiaoxian and Li, Qingfeng and Zheng, Maolin and Zhu, Jinshun and Zhang, Jing and Du, Wangqi and Chen, Jun and Zhu, Shanli and Zhang, Lifang
Journal: Applied microbiology and biotechnology (2021): 7283-7293
Simple methodology to visualize whole-brain microvasculature in three dimensions.
Authors: Khouri, Katiana and Xie, Danny F and Crouzet, Christian and Bahani, Adrian W and Cribbs, David H and Fisher, Mark J and Choi, Bernard
Journal: Neurophotonics (2021): 025004
Authors: Khouri, Katiana and Xie, Danny F and Crouzet, Christian and Bahani, Adrian W and Cribbs, David H and Fisher, Mark J and Choi, Bernard
Journal: Neurophotonics (2021): 025004
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