DiYO™-1 [equivalent to YOYO®-1]
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1270.65 |
Solvent | DMSO |
Spectral properties
Extinction coefficient (cm -1 M -1) | 989001 |
Excitation (nm) | 491 |
Emission (nm) | 508 |
Quantum yield | 0.521 |
Storage, safety and handling
H-phrase | H303, H313, H340 |
Hazard symbol | T |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R68 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 41116134 |
Alternative formats
DiYO™-1 [equivalent to YOYO®-1] *5 mM DMSO Solution* |
Overview | SDSProtocol |
See also: Nucleus, Cell Viability Assays
CAS 143413-85-8 | Molecular weight 1270.65 | Extinction coefficient (cm -1 M -1) 989001 | Excitation (nm) 491 | Emission (nm) 508 | Quantum yield 0.521 |
DiYO™-1 is chemically equivalent to YOYO®-1 (YOYO® is the trademark of Invitrogen). DiYO™-1 is a carbocyanine dimer with green fluorescence similar to FITC. It is cell-impermeant and easily distinguished from Cy5 and rhodamines as a nuclear counterstain and dead cell indicator. It is non-fluorescent in the absence of nucleic acids, and generates a very bright fluorescence signal upon binding to DNA. DiYO™-1 gives strong and selective nuclear staining in cultured cells and in paraffin sections. Simultaneous labeling with cell-permeable Nuclear Red™ LCS1 dye and cell-impermeant DiYO®-1 can be used to assess cell viability. DiYO™-1 and Nuclear Red™ both have much greater extinction coefficients than that of DNA-bound propidium iodide.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DiYO™-1 [equivalent to YOYO®-1] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 78.7 µL | 393.499 µL | 786.999 µL | 3.935 mL | 7.87 mL |
5 mM | 15.74 µL | 78.7 µL | 157.4 µL | 786.999 µL | 1.574 mL |
10 mM | 7.87 µL | 39.35 µL | 78.7 µL | 393.499 µL | 786.999 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Extinction coefficient (cm -1 M -1) | 989001 |
Excitation (nm) | 491 |
Emission (nm) | 508 |
Quantum yield | 0.521 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
DiYO™-3 [equivalent to YOYO®-3] *5 mM DMSO Solution* *CAS 156312-20-8* | 612 | 631 | 1670001 |
Oxazine 1 *CAS 24796-94-9* | 648 | 668 | 123000 |
DiTO™-1 [equivalent to TOTO®-1] *5 mM DMSO Solution* *CAS 143413-84-7* | 514 | 531 | 1170001 |
JC-1 [5,5,6,6-Tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide] *CAS#: 3520-43-2* | 515 | 530 | 1950001 |
BTA-1 [2-(4'-(methylamino)phenyl)benzothiazole] | 349 | 421 | - |
DiPO™-1 [equivalent to POPO®-1] *1 mM DMSO Solution* | 433 | 457 | 920001 |
EthD-1 [Ethidium Homodimer-1] | 528 | 617 | - |
Citations
View all 27 citations: Citation Explorer
Trellis tree-based analysis reveals stromal regulation of patient-derived organoid drug responses
Authors: Zapatero, Mar{\'\i}a Ramos and Tong, Alexander and Opzoomer, James W and O’Sullivan, Rhianna and Rodriguez, Ferran Cardoso and Sufi, Jahangir and Vlckova, Petra and Nattress, Callum and Qin, Xiao and Claus, Jeroen and others,
Journal: Cell (2023): 5606--5619
Authors: Zapatero, Mar{\'\i}a Ramos and Tong, Alexander and Opzoomer, James W and O’Sullivan, Rhianna and Rodriguez, Ferran Cardoso and Sufi, Jahangir and Vlckova, Petra and Nattress, Callum and Qin, Xiao and Claus, Jeroen and others,
Journal: Cell (2023): 5606--5619
Extracellular DNA: A Critical Aspect of Marine Biofilms
Authors: Tuck, Benjamin and Salgar-Chaparro, Silvia J and Watkin, Elizabeth and Somers, Anthony and Forsyth, Maria and Machuca, Laura L
Journal: Microorganisms (2022): 1285
Authors: Tuck, Benjamin and Salgar-Chaparro, Silvia J and Watkin, Elizabeth and Somers, Anthony and Forsyth, Maria and Machuca, Laura L
Journal: Microorganisms (2022): 1285
A viral ubiquitination switch attenuates innate immunity and triggers nuclear import of virion DNA and infection
Authors: Bauer, Michael and Gomez-Gonzalez, Alfonso and Suomalainen, Maarit and Schilling, Nicolas and Hemmi, Silvio and Greber, Urs F
Journal: Science advances (2021): eabl7150
Authors: Bauer, Michael and Gomez-Gonzalez, Alfonso and Suomalainen, Maarit and Schilling, Nicolas and Hemmi, Silvio and Greber, Urs F
Journal: Science advances (2021): eabl7150
Non-viral gene delivery of the oncotoxic protein NS1 for treatment of hepatocellular carcinoma
Authors: Witzigmann, Dominik and Grossen, Philip and Quintavalle, Cristina and Lanzafame, Manuela and Schenk, Susanne H and Tran, Xue-Ting and Englinger, Bernhard and Hauswirth, Patrick and Gr{\"u}nig, David and van Schoonhoven, Sushilla and others,
Journal: Journal of Controlled Release (2021): 138--152
Authors: Witzigmann, Dominik and Grossen, Philip and Quintavalle, Cristina and Lanzafame, Manuela and Schenk, Susanne H and Tran, Xue-Ting and Englinger, Bernhard and Hauswirth, Patrick and Gr{\"u}nig, David and van Schoonhoven, Sushilla and others,
Journal: Journal of Controlled Release (2021): 138--152
DNase-mediated eDNA removal enhances D-LL-31 activity against biofilms of bacteria isolated from chronic rhinosinusitis patients
Authors: Wongkaewkhiaw, Saharut and Kanthawong, Sakawrat and Bolscher, Jan GM and Nazmi, Kamran and Taweechaisupapong, Suwimol and Krom, Bastiaan P
Journal: Biofouling (2020): 1--12
Authors: Wongkaewkhiaw, Saharut and Kanthawong, Sakawrat and Bolscher, Jan GM and Nazmi, Kamran and Taweechaisupapong, Suwimol and Krom, Bastiaan P
Journal: Biofouling (2020): 1--12
Correction: Photobleaching of YOYO-1 in super-resolution single DNA fluorescence imaging
Authors: Pyle, J. R., Chen, J.
Journal: Beilstein J Nanotechnol (2018): 809-811
Authors: Pyle, J. R., Chen, J.
Journal: Beilstein J Nanotechnol (2018): 809-811
Ultrafast Transient Absorption Spectra of Photoexcited YOYO-1 molecules call for additional investigations of their fluorescence quenching mechanism
Authors: Wang, L., Pyle, J. R., Cimatu, K. A., Chen, J.
Journal: J Photochem Photobiol A Chem (2018): 411-419
Authors: Wang, L., Pyle, J. R., Cimatu, K. A., Chen, J.
Journal: J Photochem Photobiol A Chem (2018): 411-419
Photobleaching of YOYO-1 in super-resolution single DNA fluorescence imaging
Authors: Pyle, J. R., Chen, J.
Journal: Beilstein J Nanotechnol (2017): 2296-2306
Authors: Pyle, J. R., Chen, J.
Journal: Beilstein J Nanotechnol (2017): 2296-2306
Detection of amyloid-beta fibrils using the DNA-intercalating dye YOYO-1: Binding mode and fibril formation kinetics
Authors: Lindberg, D. J., Esbjorner, E. K.
Journal: Biochem Biophys Res Commun (2016): 313-8
Authors: Lindberg, D. J., Esbjorner, E. K.
Journal: Biochem Biophys Res Commun (2016): 313-8
Interference of ATP with the fluorescent probes YOYO-1 andYOYO-3 modifies the mechanical properties of intercalator-stained DNA confined in nanochannels
Authors: Roushan, M., Azad, Z., Lim, S. F., Wang, H., Riehn, R.
Journal: Mikrochim Acta (2015): 1561-1565
Authors: Roushan, M., Azad, Z., Lim, S. F., Wang, H., Riehn, R.
Journal: Mikrochim Acta (2015): 1561-1565
References
View all 22 references: Citation Explorer
Plasmalemma permeability and necrotic cell death phenotypes after intracerebral hemorrhage in mice
Authors: Zhu X, Tao L, Tejima-M and eville E, Qiu J, Park J, Garber K, Ericsson M, Lo EH, Whalen MJ.
Journal: Stroke (2012): 524
Authors: Zhu X, Tao L, Tejima-M and eville E, Qiu J, Park J, Garber K, Ericsson M, Lo EH, Whalen MJ.
Journal: Stroke (2012): 524
Intra-organ Biodistribution of Gold Nanoparticles Using Intrinsic Two-photon Induced Photoluminescence
Authors: Park J, Estrada A, Schwartz JA, Diagaradjane P, Krishnan S, Dunn AK, Tunnell JW.
Journal: Lasers Surg Med (2010): 630
Authors: Park J, Estrada A, Schwartz JA, Diagaradjane P, Krishnan S, Dunn AK, Tunnell JW.
Journal: Lasers Surg Med (2010): 630
Screening by imaging: scaling up single-DNA-molecule analysis with a novel parabolic VA-TIRF reflector and noise-reduction techniques
Authors: van 't Hoff M, Reuter M, Dryden DT, Oheim M.
Journal: Phys Chem Chem Phys (2009): 7713
Authors: van 't Hoff M, Reuter M, Dryden DT, Oheim M.
Journal: Phys Chem Chem Phys (2009): 7713
Novel anatomic structures in the brain and spinal cord of rabbit that may belong to the Bonghan system of potential acupuncture meridians
Authors: Lee BC, Kim S, Soh KS.
Journal: J Acupunct Meridian Stud (2008): 29
Authors: Lee BC, Kim S, Soh KS.
Journal: J Acupunct Meridian Stud (2008): 29
Triplet fraction buildup effect of the DNA-YOYO complex studied with fluorescence correlation spectroscopy
Authors: Shimizu M, Sasaki S, Kinjo M.
Journal: Anal Biochem (2007): 87
Authors: Shimizu M, Sasaki S, Kinjo M.
Journal: Anal Biochem (2007): 87
DNA length evaluation using cyanine dye and fluorescence correlation spectroscopy
Authors: Shimizu M, Sasaki S, Tsuruoka M.
Journal: Biomacromolecules (2005): 2703
Authors: Shimizu M, Sasaki S, Tsuruoka M.
Journal: Biomacromolecules (2005): 2703
Implementation of accurate and fast DNA cytometry by confocal microscopy in 3D
Authors: Ploeger LS, Huisman A, van der Gugten J, van der Giezen DM, Belien JA, Abbaker AY, Dullens HF, Grizzle W, Poulin NM, Meijer GA, van Diest PJ.
Journal: Cell Oncol (2005): 225
Authors: Ploeger LS, Huisman A, van der Gugten J, van der Giezen DM, Belien JA, Abbaker AY, Dullens HF, Grizzle W, Poulin NM, Meijer GA, van Diest PJ.
Journal: Cell Oncol (2005): 225
TO-PRO-3 is an optimal fluorescent dye for nuclear counterstaining in dual-colour FISH on paraffin sections
Authors: Bink K, Walch A, Feuchtinger A, Eisenmann H, Hutzler P, Hofler H, Werner M.
Journal: Histochem Cell Biol (2001): 293
Authors: Bink K, Walch A, Feuchtinger A, Eisenmann H, Hutzler P, Hofler H, Werner M.
Journal: Histochem Cell Biol (2001): 293
Green-light transilluminator for the detection without photodamage of proteins and DNA labeled with different fluorescent dyes
Authors: Alba FJ, Bermudez A, Daban JR.
Journal: Electrophoresis (2001): 399
Authors: Alba FJ, Bermudez A, Daban JR.
Journal: Electrophoresis (2001): 399
Photophysical properties of fluorescent DNA-dyes bound to single- and double-stranded DNA in aqueous buffered solution
Authors: Cosa G, Focsaneanu KS, McLean JR, McNamee JP, Scaiano JC.
Journal: Photochem Photobiol (2001): 585
Authors: Cosa G, Focsaneanu KS, McLean JR, McNamee JP, Scaiano JC.
Journal: Photochem Photobiol (2001): 585
Application notes
FAQ
What are the most popular green DNA dyes?
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
Are mRNAs found in prokaryotes differ from those of eukaryotes?
Are spliceosomes associated with any diseases?
Are there any alternatives for ethidium bromide in agarose gels?
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
Are mRNAs found in prokaryotes differ from those of eukaryotes?
Are spliceosomes associated with any diseases?
Are there any alternatives for ethidium bromide in agarose gels?