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DiI Dye
1,1-Dioctadecyl-3,3,3,3- tetramethylindocarbocyanine iodide
DiI, DiO, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures. The fluorescence of these environment-sensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins although they are weakly fluorescent in water. They have high extinction coefficients, polarity-dependent fluorescence and short excited-state lifetimes. Once applied to cells, these dyes diffuse laterally within the cellular plasma membranes, resulting in even staining of the entire cell at their optimal concentrations. The distinct fluorescence colors of DiI (orange fluorescence), DiO (green fluorescence), DiD (red fluorescence) and DiR (deep red fluorescent) provide a convenient tool for multicolor imaging and flow cytometric analysis of live cells. DiO and DiI can be used with standard FITC and TRITC filters respectively. Among them DiI and its analogs are most frequently used since they usually exhibit very low cell toxicity. In addition, DiI is widely used for determining lipoproteins such as LDL and HDL.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DiI iodide [1,1-Dioctadecyl-3,3,3,3- tetramethylindocarbocyanine iodide] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 104.024 µL | 520.118 µL | 1.04 mL | 5.201 mL | 10.402 mL |
| 5 mM | 20.805 µL | 104.024 µL | 208.047 µL | 1.04 mL | 2.08 mL |
| 10 mM | 10.402 µL | 52.012 µL | 104.024 µL | 520.118 µL | 1.04 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| Propidium iodide *CAS 25535-16-4* | 537 | 618 | 60001 |
| Propidium iodide *10 mM aqueous solution* | 537 | 618 | 60001 |
| DiR iodide [1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide] | 754 | 778 | 2700001 |
| DiI perchlorate [1,1-Dioctadecyl-3,3,3,3-tetramethylindocarbocyanine perchlorate] *CAS 41085-99-8* | 550 | 564 | 148000 |
| DiI triflate [1,1-Dioctadecyl-3,3,3,3-tetramethylindocarbocyanine triflate] | 550 | 564 | 148000 |
| Propidium iodide *1 mg/mL aqueous solution* | 537 | 618 | 60001 |
Citations
View all 53 citations: Citation Explorer
Engineering Central Substitutions in Heptamethine Dyes for Improved Fluorophore Performance
Authors: Guo, Lei and Yang, Meek and Dong, Bin and Lewman, Seth and Van Horn, Alex and Jia, Shang
Journal: JACS Au (2024)
Authors: Guo, Lei and Yang, Meek and Dong, Bin and Lewman, Seth and Van Horn, Alex and Jia, Shang
Journal: JACS Au (2024)
Modular strategy for development of the hierarchical visual network in mice
Authors: Murakami, Tomonari and Matsui, Teppei and Uemura, Masato and Ohki, Kenichi
Journal: Nature (2022): 578--585
Authors: Murakami, Tomonari and Matsui, Teppei and Uemura, Masato and Ohki, Kenichi
Journal: Nature (2022): 578--585
Extracellular vesicles from lung tissue drive bone marrow neutrophil recruitment in inflammation
Authors: Liu, Bowen and Jin, Yuan and Yang, Jingyi and Han, Yue and Shan, Hui and Qiu, Mantang and Zhao, Xuyang and Liu, Anhang and Jin, Yan and Yin, Yuxin
Journal: Journal of Extracellular Vesicles (2022): e12223
Authors: Liu, Bowen and Jin, Yuan and Yang, Jingyi and Han, Yue and Shan, Hui and Qiu, Mantang and Zhao, Xuyang and Liu, Anhang and Jin, Yan and Yin, Yuxin
Journal: Journal of Extracellular Vesicles (2022): e12223
A novel strategy to identify candidate diagnostic and prognostic biomarkers for gastric cancer
Authors: Liu, Lei and Pang, Honglin and He, Qiao and Pan, Biran and Sun, Xiaobin and Shan, Jing and Wu, Liping and Wu, Kaiwen and Yao, Xue and Guo, Yuanbiao
Journal: Cancer Cell International (2021): 1--15
Authors: Liu, Lei and Pang, Honglin and He, Qiao and Pan, Biran and Sun, Xiaobin and Shan, Jing and Wu, Liping and Wu, Kaiwen and Yao, Xue and Guo, Yuanbiao
Journal: Cancer Cell International (2021): 1--15
Insight into the in vivo translocation of oral liposomes by fluorescence resonance energy transfer effect
Authors: Liu, Wenjuan and Li, Dong and Dong, Zirong and Liu, Kaiheng and He, Haisheng and Lu, Yi and Wu, Wei and Li, Qinghua and Gan, Li and Qi, Jianping
Journal: International Journal of Pharmaceutics (2020): 119682
Authors: Liu, Wenjuan and Li, Dong and Dong, Zirong and Liu, Kaiheng and He, Haisheng and Lu, Yi and Wu, Wei and Li, Qinghua and Gan, Li and Qi, Jianping
Journal: International Journal of Pharmaceutics (2020): 119682
References
View all 92 references: Citation Explorer
Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing
Authors: Kalchenko V, Shivtiel S, Malina V, Lapid K, Haramati S, Lapidot T, Brill A, Harmelin A.
Journal: J Biomed Opt (2006): 50507
Authors: Kalchenko V, Shivtiel S, Malina V, Lapid K, Haramati S, Lapidot T, Brill A, Harmelin A.
Journal: J Biomed Opt (2006): 50507
Nox2, Ca2+, and protein kinase C play a role in angiotensin II-induced free radical production in nucleus tractus solitarius
Authors: Wang G, Anrather J, Glass MJ, Tarsitano MJ, Zhou P, Frys KA, Pickel VM, Iadecola C.
Journal: Hypertension (2006): 482
Authors: Wang G, Anrather J, Glass MJ, Tarsitano MJ, Zhou P, Frys KA, Pickel VM, Iadecola C.
Journal: Hypertension (2006): 482
Functional neuroanatomy of the rhinophore of Aplysia punctata
Authors: Wertz A, Rossler W, Obermayer M, Bickmeyer U.
Journal: Front Zool (2006): 6
Authors: Wertz A, Rossler W, Obermayer M, Bickmeyer U.
Journal: Front Zool (2006): 6
In vivo imaging and counting of rat retinal ganglion cells using a scanning laser ophthalmoscope
Authors: Higashide T, Kawaguchi I, Ohkubo S, Takeda H, Sugiyama K.
Journal: Invest Ophthalmol Vis Sci (2006): 2943
Authors: Higashide T, Kawaguchi I, Ohkubo S, Takeda H, Sugiyama K.
Journal: Invest Ophthalmol Vis Sci (2006): 2943
Confocal laser scanning microscopy using dialkylcarbocyanine dyes for cell tracing in hard and soft biomaterials
Authors: Heinrich L, Freyria AM, Melin M, Tourneur Y, Maksoud R, Bernengo JC, Hartmann DJ.
Journal: J Biomed Mater Res B Appl Biomater. (2006)
Authors: Heinrich L, Freyria AM, Melin M, Tourneur Y, Maksoud R, Bernengo JC, Hartmann DJ.
Journal: J Biomed Mater Res B Appl Biomater. (2006)
Page updated on August 13, 2024
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