DiD Perchlorate *UltraPure Grade*
Ordering information
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Additional ordering information
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Fax | 1-800-609-2943 |
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International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 959.91 |
Solvent | DMSO |
Spectral properties
Extinction coefficient (cm -1 M -1) | 260000 |
Excitation (nm) | 646 |
Emission (nm) | 663 |
Storage, safety and handling
Certificate of Origin | Download PDF |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | ![]() ![]() |
See also: Plasma Membrane, Exosomes
CAS 127274-91-3 | Molecular weight 959.91 | Extinction coefficient (cm -1 M -1) 260000 | Excitation (nm) 646 | Emission (nm) 663 |
DiI, DiO, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures. The fluorescence of these environment-sensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins although they are weakly fluorescent in water. They have high extinction coefficients, polarity-dependent fluorescence and short excited-state lifetimes. Once applied to cells, these dyes diffuse laterally within the cellular plasma membranes, resulting in even staining of the entire cell at their optimal concentrations. The distinct fluorescence colors of DiI (orange fluorescence), DiO (green fluorescence), DiD (red fluorescence) and DiR (deep red fluorescent) provide a convenient tool for multicolor imaging and flow cytometric analysis of live cells. DiO and DiI can be used with standard FITC and TRITC filters respectively. Among them DiD is well excited by the 633 nm He-Ne laser, and has much longer excitation and emission wavelengths than those of DiI, providing a valuable alternative for labeling cells and tissues that have significant intrinsic fluorescence. DiR might be useful for in vivo imaging or tracing due to the effective transmission of infrared light through cells and tissues and low level of autofluorescence in the infrared range.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DiD Perchlorate *UltraPure Grade* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 104.176 µL | 520.882 µL | 1.042 mL | 5.209 mL | 10.418 mL |
5 mM | 20.835 µL | 104.176 µL | 208.353 µL | 1.042 mL | 2.084 mL |
10 mM | 10.418 µL | 52.088 µL | 104.176 µL | 520.882 µL | 1.042 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
![spectrum](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fspectra%2Fdid.png&w=2048&q=50)
![spectrum](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fspectra%2Fdid.png&w=2048&q=50)
Spectral properties
Extinction coefficient (cm -1 M -1) | 260000 |
Excitation (nm) | 646 |
Emission (nm) | 663 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
DiO perchlorate [3,3-Dioctadecyloxacarbocyanine perchlorate] *CAS#: 34215-57-1* | 483 | 501 | - |
DiI perchlorate [1,1-Dioctadecyl-3,3,3,3-tetramethylindocarbocyanine perchlorate] *CAS 41085-99-8* | 550 | 564 | 148000 |
Citations
View all 53 citations: Citation Explorer
Observation of antitumor mechanism of GE11-modified paclitaxel and curcumin liposomes based on cellular morphology changes
Authors: Tang, Hailing and Li, Lijuan and Wang, Baoshan and Guangxi Scientific Research Center of Traditional Chinese Medicine,
Journal: AAPS Open (2024): 1
Authors: Tang, Hailing and Li, Lijuan and Wang, Baoshan and Guangxi Scientific Research Center of Traditional Chinese Medicine,
Journal: AAPS Open (2024): 1
An oncolytic virus--T cell chimera for cancer immunotherapy
Authors: Chen, Yuxuan and Chen, Xiaohong and Bao, Weier and Liu, Gang and Wei, Wei and Ping, Yuan
Journal: Nature Biotechnology (2024): 1--12
Authors: Chen, Yuxuan and Chen, Xiaohong and Bao, Weier and Liu, Gang and Wei, Wei and Ping, Yuan
Journal: Nature Biotechnology (2024): 1--12
Two-Pronged Intracellular Co-Delivery of Antigen and Adjuvant for Synergistic Cancer Immunotherapy
Authors: Meng, Junli and Zhang, Peisen and Chen, Qizhe and Wang, Zihua and Gu, Yuan and Ma, Jie and Li, Wang and Yang, Chen and Qiao, Yuanyuan and Hou, Yi and others,
Journal: Advanced Materials (2022): 2202168
Authors: Meng, Junli and Zhang, Peisen and Chen, Qizhe and Wang, Zihua and Gu, Yuan and Ma, Jie and Li, Wang and Yang, Chen and Qiao, Yuanyuan and Hou, Yi and others,
Journal: Advanced Materials (2022): 2202168
Insight into the in vivo translocation of oral liposomes by fluorescence resonance energy transfer effect
Authors: Liu, Wenjuan and Li, Dong and Dong, Zirong and Liu, Kaiheng and He, Haisheng and Lu, Yi and Wu, Wei and Li, Qinghua and Gan, Li and Qi, Jianping
Journal: International Journal of Pharmaceutics (2020): 119682
Authors: Liu, Wenjuan and Li, Dong and Dong, Zirong and Liu, Kaiheng and He, Haisheng and Lu, Yi and Wu, Wei and Li, Qinghua and Gan, Li and Qi, Jianping
Journal: International Journal of Pharmaceutics (2020): 119682
Directional Migration and Odontogenic Differentiation of Bone Marrow Stem Cells Induced by Dentin Coated with Nanobioactive Glass
Authors: Wang, Sainan and Huang, Guibin and Dong, Yanmei
Journal: Journal of Endodontics (2019)
Authors: Wang, Sainan and Huang, Guibin and Dong, Yanmei
Journal: Journal of Endodontics (2019)
The Influence of Cell Source and Donor Age on the Tenogenic Potential and Chemokine Secretion of Human Mesenchymal Stromal Cells
Authors: Zarychta-Wisniewska, Weronika and Burdzińska, Anna and Zielniok, Katarzyna and Koblowska, Marta and Gala, Kamila and Pedzisz, Piotr and Iwanicka-Nowicka, Roksana and Fogtman, Anna and Aksamit, Aleks and ra , undefined and Kulesza, Agnieszka and others, undefined
Journal: Stem Cells International (2019)
Authors: Zarychta-Wisniewska, Weronika and Burdzińska, Anna and Zielniok, Katarzyna and Koblowska, Marta and Gala, Kamila and Pedzisz, Piotr and Iwanicka-Nowicka, Roksana and Fogtman, Anna and Aksamit, Aleks and ra , undefined and Kulesza, Agnieszka and others, undefined
Journal: Stem Cells International (2019)
Loss of Integrity of Doxorubicin Liposomes during Transcellular Transportation Evidenced by Fluorescence Resonance Energy Transfer Effect
Authors: Li, Dong and Zhuang, Jie and Yang, Yinqian and Wang, D and an , undefined and Yang, Jinlong and He, Haisheng and Fan, Wufa and Banerjee, Amrita and Lu, Yi and Wu, Wei and others, undefined
Journal: Colloids and Surfaces B: Biointerfaces (2018)
Authors: Li, Dong and Zhuang, Jie and Yang, Yinqian and Wang, D and an , undefined and Yang, Jinlong and He, Haisheng and Fan, Wufa and Banerjee, Amrita and Lu, Yi and Wu, Wei and others, undefined
Journal: Colloids and Surfaces B: Biointerfaces (2018)
Intraurethral co-transplantation of bone marrow mesenchymal stem cells and muscle-derived cells improves the urethral closure
Authors: Burdzinska, Anna and Dybowski, Bartosz and Zarychta-Wisniewska, Weronika and Kulesza, Agnieszka and Butrym, Marta and Zagozdzon, Radoslaw and Graczyk-Jarzynka, Agnieszka and Radziszewski, Piotr and Gajewski, Zdzislaw and Paczek, Leszek
Journal: Stem cell research & therapy (2018): 239
Authors: Burdzinska, Anna and Dybowski, Bartosz and Zarychta-Wisniewska, Weronika and Kulesza, Agnieszka and Butrym, Marta and Zagozdzon, Radoslaw and Graczyk-Jarzynka, Agnieszka and Radziszewski, Piotr and Gajewski, Zdzislaw and Paczek, Leszek
Journal: Stem cell research & therapy (2018): 239
On-demand drug release from dual-targeting small nanoparticles triggered by high-intensity focused ultrasound enhanced glioblastoma-targeting therapy
Authors: Luo, Zimiao and Jin, Kai and Pang, Qiang and Shen, Shun and Yan, Zhiqiang and Jiang, Ting and Zhu, Xiaoyan and Yu, Lei and Pang, Zhiqing and Jiang, Xinguo
Journal: ACS applied materials \& interfaces (2017): 31612--31625
Authors: Luo, Zimiao and Jin, Kai and Pang, Qiang and Shen, Shun and Yan, Zhiqiang and Jiang, Ting and Zhu, Xiaoyan and Yu, Lei and Pang, Zhiqing and Jiang, Xinguo
Journal: ACS applied materials \& interfaces (2017): 31612--31625
On-Demand Drug Releasing from Dual Targeting Small Nanoparticles Triggered by High Intensity Focused Ultrasound Enhanced Glioblastoma Targeting Therapy
Authors: Luo, Zimiao and Jin, Kai and Pang, Qiang and shen, shun and Yan, Zhiqiang and Jiang, Ting and Zhu, Xiaoyan and Yu, Lei and Pang, Zhiqing and Jiang, Xinguo
Journal: ACS Applied Materials & Interfaces (2017)
Authors: Luo, Zimiao and Jin, Kai and Pang, Qiang and shen, shun and Yan, Zhiqiang and Jiang, Ting and Zhu, Xiaoyan and Yu, Lei and Pang, Zhiqing and Jiang, Xinguo
Journal: ACS Applied Materials & Interfaces (2017)
References
View all 92 references: Citation Explorer
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Journal: Front Zool (2006): 6
Authors: Wertz A, Rossler W, Obermayer M, Bickmeyer U.
Journal: Front Zool (2006): 6
Confocal laser scanning microscopy using dialkylcarbocyanine dyes for cell tracing in hard and soft biomaterials
Authors: Heinrich L, Freyria AM, Melin M, Tourneur Y, Maksoud R, Bernengo JC, Hartmann DJ.
Journal: J Biomed Mater Res B Appl Biomater. (2006)
Authors: Heinrich L, Freyria AM, Melin M, Tourneur Y, Maksoud R, Bernengo JC, Hartmann DJ.
Journal: J Biomed Mater Res B Appl Biomater. (2006)
In vivo imaging and counting of rat retinal ganglion cells using a scanning laser ophthalmoscope
Authors: Higashide T, Kawaguchi I, Ohkubo S, Takeda H, Sugiyama K.
Journal: Invest Ophthalmol Vis Sci (2006): 2943
Authors: Higashide T, Kawaguchi I, Ohkubo S, Takeda H, Sugiyama K.
Journal: Invest Ophthalmol Vis Sci (2006): 2943
Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing
Authors: Kalchenko V, Shivtiel S, Malina V, Lapid K, Haramati S, Lapidot T, Brill A, Harmelin A.
Journal: J Biomed Opt (2006): 50507
Authors: Kalchenko V, Shivtiel S, Malina V, Lapid K, Haramati S, Lapidot T, Brill A, Harmelin A.
Journal: J Biomed Opt (2006): 50507
Nox2, Ca2+, and protein kinase C play a role in angiotensin II-induced free radical production in nucleus tractus solitarius
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Journal: Hypertension (2006): 482
Regional cardiac ganglia projections in the guinea pig heart studied by postmortem DiI tracing
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Authors: Harrison TA, Perry KM, Hoover DB.
Journal: Anat Rec A Discov Mol Cell Evol Biol (2005): 758
Anatomic relationships of the human nucleus of the solitary tract in the medulla oblongata: a DiI labeling study
Authors: Zec N, Kinney HC.
Journal: Auton Neurosci (2003): 131
Authors: Zec N, Kinney HC.
Journal: Auton Neurosci (2003): 131
Projection pattern of nerve fibers from the septal organ: DiI-tracing studies with transgenic OMP mice
Authors: Levai O, Strotmann J.
Journal: Histochem Cell Biol (2003): 483
Authors: Levai O, Strotmann J.
Journal: Histochem Cell Biol (2003): 483
Retinal ganglion cells resistant to advanced glaucoma: a postmortem study of human retinas with the carbocyanine dye DiI
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Journal: Invest Ophthalmol Vis Sci (2003): 5196
Authors: Pavlidis M, Stupp T, Naskar R, Cengiz C, Thanos S.
Journal: Invest Ophthalmol Vis Sci (2003): 5196
A novel morphological technique to investigate a single climbing fibre synaptogenesis with a Purkinje cell in the developing mouse cerebellum: DiI injection into the inferior cerebellar peduncle
Authors: Kiyohara Y, Endo K, Ide C, Mizoguchi A.
Journal: J Electron Microsc (Tokyo) (2003): 327
Authors: Kiyohara Y, Endo K, Ide C, Mizoguchi A.
Journal: J Electron Microsc (Tokyo) (2003): 327