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DiD Dye
UltraPure Grade
DiI, DiO, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures. The fluorescence of these environment-sensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins although they are weakly fluorescent in water. They have high extinction coefficients, polarity-dependent fluorescence and short excited-state lifetimes. Once applied to cells, these dyes diffuse laterally within the cellular plasma membranes, resulting in even staining of the entire cell at their optimal concentrations. The distinct fluorescence colors of DiI (orange fluorescence), DiO (green fluorescence), DiD (red fluorescence) and DiR (deep red fluorescent) provide a convenient tool for multicolor imaging and flow cytometric analysis of live cells. DiO and DiI can be used with standard FITC and TRITC filters respectively. Among them DiD is well excited by the 633 nm He-Ne laser, and has much longer excitation and emission wavelengths than those of DiI, providing a valuable alternative for labeling cells and tissues that have significant intrinsic fluorescence. DiR might be useful for in vivo imaging or tracing due to the effective transmission of infrared light through cells and tissues and low level of autofluorescence in the infrared range.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DiD Perchlorate *UltraPure Grade* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 104.176 µL | 520.882 µL | 1.042 mL | 5.209 mL | 10.418 mL |
| 5 mM | 20.835 µL | 104.176 µL | 208.353 µL | 1.042 mL | 2.084 mL |
| 10 mM | 10.418 µL | 52.088 µL | 104.176 µL | 520.882 µL | 1.042 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| DiO perchlorate [3,3-Dioctadecyloxacarbocyanine perchlorate] *CAS#: 34215-57-1* | 483 | 501 | - |
| DiI perchlorate [1,1-Dioctadecyl-3,3,3,3-tetramethylindocarbocyanine perchlorate] *CAS 41085-99-8* | 550 | 564 | 148000 |
Citations
View all 54 citations: Citation Explorer
In situ reprogramming of tumor-associated macrophages enhances cancer immunotherapy
Authors: Han, Yanjie and Lu, Haifeng and Gu, Zikuan and Guan, Peixin and Liu, Zhen
Journal: Nano Today (2024): 102420
Authors: Han, Yanjie and Lu, Haifeng and Gu, Zikuan and Guan, Peixin and Liu, Zhen
Journal: Nano Today (2024): 102420
Observation of antitumor mechanism of GE11-modified paclitaxel and curcumin liposomes based on cellular morphology changes
Authors: Tang, Hailing and Li, Lijuan and Wang, Baoshan and Guangxi Scientific Research Center of Traditional Chinese Medicine,
Journal: AAPS Open (2024): 1
Authors: Tang, Hailing and Li, Lijuan and Wang, Baoshan and Guangxi Scientific Research Center of Traditional Chinese Medicine,
Journal: AAPS Open (2024): 1
An oncolytic virus--T cell chimera for cancer immunotherapy
Authors: Chen, Yuxuan and Chen, Xiaohong and Bao, Weier and Liu, Gang and Wei, Wei and Ping, Yuan
Journal: Nature Biotechnology (2024): 1--12
Authors: Chen, Yuxuan and Chen, Xiaohong and Bao, Weier and Liu, Gang and Wei, Wei and Ping, Yuan
Journal: Nature Biotechnology (2024): 1--12
Two-Pronged Intracellular Co-Delivery of Antigen and Adjuvant for Synergistic Cancer Immunotherapy
Authors: Meng, Junli and Zhang, Peisen and Chen, Qizhe and Wang, Zihua and Gu, Yuan and Ma, Jie and Li, Wang and Yang, Chen and Qiao, Yuanyuan and Hou, Yi and others,
Journal: Advanced Materials (2022): 2202168
Authors: Meng, Junli and Zhang, Peisen and Chen, Qizhe and Wang, Zihua and Gu, Yuan and Ma, Jie and Li, Wang and Yang, Chen and Qiao, Yuanyuan and Hou, Yi and others,
Journal: Advanced Materials (2022): 2202168
Insight into the in vivo translocation of oral liposomes by fluorescence resonance energy transfer effect
Authors: Liu, Wenjuan and Li, Dong and Dong, Zirong and Liu, Kaiheng and He, Haisheng and Lu, Yi and Wu, Wei and Li, Qinghua and Gan, Li and Qi, Jianping
Journal: International Journal of Pharmaceutics (2020): 119682
Authors: Liu, Wenjuan and Li, Dong and Dong, Zirong and Liu, Kaiheng and He, Haisheng and Lu, Yi and Wu, Wei and Li, Qinghua and Gan, Li and Qi, Jianping
Journal: International Journal of Pharmaceutics (2020): 119682
References
View all 92 references: Citation Explorer
Functional neuroanatomy of the rhinophore of Aplysia punctata
Authors: Wertz A, Rossler W, Obermayer M, Bickmeyer U.
Journal: Front Zool (2006): 6
Authors: Wertz A, Rossler W, Obermayer M, Bickmeyer U.
Journal: Front Zool (2006): 6
Confocal laser scanning microscopy using dialkylcarbocyanine dyes for cell tracing in hard and soft biomaterials
Authors: Heinrich L, Freyria AM, Melin M, Tourneur Y, Maksoud R, Bernengo JC, Hartmann DJ.
Journal: J Biomed Mater Res B Appl Biomater. (2006)
Authors: Heinrich L, Freyria AM, Melin M, Tourneur Y, Maksoud R, Bernengo JC, Hartmann DJ.
Journal: J Biomed Mater Res B Appl Biomater. (2006)
In vivo imaging and counting of rat retinal ganglion cells using a scanning laser ophthalmoscope
Authors: Higashide T, Kawaguchi I, Ohkubo S, Takeda H, Sugiyama K.
Journal: Invest Ophthalmol Vis Sci (2006): 2943
Authors: Higashide T, Kawaguchi I, Ohkubo S, Takeda H, Sugiyama K.
Journal: Invest Ophthalmol Vis Sci (2006): 2943
Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing
Authors: Kalchenko V, Shivtiel S, Malina V, Lapid K, Haramati S, Lapidot T, Brill A, Harmelin A.
Journal: J Biomed Opt (2006): 50507
Authors: Kalchenko V, Shivtiel S, Malina V, Lapid K, Haramati S, Lapidot T, Brill A, Harmelin A.
Journal: J Biomed Opt (2006): 50507
Nox2, Ca2+, and protein kinase C play a role in angiotensin II-induced free radical production in nucleus tractus solitarius
Authors: Wang G, Anrather J, Glass MJ, Tarsitano MJ, Zhou P, Frys KA, Pickel VM, Iadecola C.
Journal: Hypertension (2006): 482
Authors: Wang G, Anrather J, Glass MJ, Tarsitano MJ, Zhou P, Frys KA, Pickel VM, Iadecola C.
Journal: Hypertension (2006): 482
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