DiO perchlorate [3,3-Dioctadecyloxacarbocyanine perchlorate] *CAS#: 34215-57-1*
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Additional ordering information
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Physical properties
Molecular weight | 881.7 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 483 |
Emission (nm) | 501 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | ![]() ![]() |
See also: Cell/Cytoplasmic Membrane Potential Activity & Analysis, Plasma Membrane, Exosomes, Membrane Potential and Channels, Neurodegeneration & Amyloid Staining
CAS 34215-57-1 | Molecular weight 881.7 | Excitation (nm) 483 | Emission (nm) 501 |
DiI, DiO, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures. The fluorescence of these environment-sensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins although they are weakly fluorescent in water. They have high extinction coefficients, polarity-dependent fluorescence and short excited-state lifetimes. Once applied to cells, these dyes diffuse laterally within the cellular plasma membranes, resulting in even staining of the entire cell at their optimal concentrations. The distinct fluorescence colors of DiI (orange fluorescence), DiO (green fluorescence), DiD (red fluorescence) and DiR (deep red fluorescent) provide a convenient tool for multicolor imaging and flow cytometric analysis of live cells. DiO and DiI can be used with standard FITC and TRITC filters respectively. Among them DiD is well excited by the 633 nm He-Ne laser, and has much longer excitation and emission wavelengths than those of DiI, providing a valuable alternative for labeling cells and tissues that have significant intrinsic fluorescence. DiR might be useful for in vivo imaging or tracing due to the effective transmission of infrared light through cells and tissues and low level of autofluorescence in the infrared range.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DiO perchlorate [3,3-Dioctadecyloxacarbocyanine perchlorate] *CAS#: 34215-57-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 113.417 µL | 567.086 µL | 1.134 mL | 5.671 mL | 11.342 mL |
5 mM | 22.683 µL | 113.417 µL | 226.835 µL | 1.134 mL | 2.268 mL |
10 mM | 11.342 µL | 56.709 µL | 113.417 µL | 567.086 µL | 1.134 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
DiD Perchlorate *UltraPure Grade* | 646 | 663 | 260000 |
DiI perchlorate [1,1-Dioctadecyl-3,3,3,3-tetramethylindocarbocyanine perchlorate] *CAS 41085-99-8* | 550 | 564 | 148000 |
Citations
View all 50 citations: Citation Explorer
Lipopolysaccharide modification enhances the inhibitory effect of clodronate liposomes on hepatic fibrosis by depletion of macrophages and hepatic stellate cells
Authors: Zhang, Luyao and Tao, Min and Zhang, Hengyan and Zhang, Shichao and Hou, Xiaojuan and Zong, Chen and Sun, Gangqi and Feng, Shiyao and Yan, Haixin and Lu, Ying and others,
Journal: Chemico-Biological Interactions (2024): 111015
Authors: Zhang, Luyao and Tao, Min and Zhang, Hengyan and Zhang, Shichao and Hou, Xiaojuan and Zong, Chen and Sun, Gangqi and Feng, Shiyao and Yan, Haixin and Lu, Ying and others,
Journal: Chemico-Biological Interactions (2024): 111015
Exosomes Secreted by CSFV-Infected Cells Evade Neutralizing Antibody to Activate Innate Immune Responses and Establish Productive Infection in Recipient Cells
Authors: Bao, Xi and Zhuang, Tenhan and Xu, Yue and Chen, Li and Feng, Lei and Yao, Huochun
Journal: Veterinary Microbiology (2024): 110062
Authors: Bao, Xi and Zhuang, Tenhan and Xu, Yue and Chen, Li and Feng, Lei and Yao, Huochun
Journal: Veterinary Microbiology (2024): 110062
Bioactive cytomembrane@ poly (citrate-peptide)-miRNA365 nanoplatform with immune escape and homologous targeting for colon cancer therapy
Authors: Zhang, Long and Zhang, Wan and Peng, Hang and Shen, Tianli and Wang, Min and Luo, Meng and Qu, Xiaoyan and Qu, Fengyi and Liu, Wenguang and Lei, Bo and others,
Journal: Materials Today Bio (2022): 100294
Authors: Zhang, Long and Zhang, Wan and Peng, Hang and Shen, Tianli and Wang, Min and Luo, Meng and Qu, Xiaoyan and Qu, Fengyi and Liu, Wenguang and Lei, Bo and others,
Journal: Materials Today Bio (2022): 100294
Glomerular Signals Underlying Olfactory Navigation
Authors: Nouboussi Nkenfack, Nelly Merveille
Journal: (2020)
Authors: Nouboussi Nkenfack, Nelly Merveille
Journal: (2020)
Design of Hydrolytically Degradable Polyethylene Glycol Crosslinkers for Facile Control of Hydrogel Degradation
Authors: Kroger, Stephanie M and Hill, Lindsay and Jain, Era and Stock, Aaron and Bracher, Paul J and He, Fahu and Zustiak, Silviya P
Journal: Macromolecular Bioscience (2020): 2000085
Authors: Kroger, Stephanie M and Hill, Lindsay and Jain, Era and Stock, Aaron and Bracher, Paul J and He, Fahu and Zustiak, Silviya P
Journal: Macromolecular Bioscience (2020): 2000085
Myosin VII, USH1C, and ANKS4B or USH1G Together Form Condensed Molecular Assembly via Liquid-Liquid Phase Separation
Authors: He, Yunyun and Li, Jianchao and Zhang, Mingjie
Journal: Cell reports (2019): 974--986
Authors: He, Yunyun and Li, Jianchao and Zhang, Mingjie
Journal: Cell reports (2019): 974--986
Cyclic RGD peptide-modified liposomal drug delivery system for targeted oral apatinib administration: enhanced cellular uptake and improved therapeutic effects
Authors: Song, Zhiwang and Lin, Yun and Xia Zhang, Chan Feng and Lu, Yonglin and Gao, Yong and Dong, Chunyan
Journal: International Journal of Nanomedicine (2017): 1941
Authors: Song, Zhiwang and Lin, Yun and Xia Zhang, Chan Feng and Lu, Yonglin and Gao, Yong and Dong, Chunyan
Journal: International Journal of Nanomedicine (2017): 1941
Influence of Particle Geometry on Gastrointestinal Transit and Absorption following Oral Administration
Authors: Li, Dong and Zhuang, Jie and He, Haisheng and Jiang, Sifan and Banerjee, Amrita and Lu, Yi and Wu, Wei and Mitragotri, Samir and Gan, Li and Qi, Jianping
Journal: ACS Applied Materials & Interfaces (2017)
Authors: Li, Dong and Zhuang, Jie and He, Haisheng and Jiang, Sifan and Banerjee, Amrita and Lu, Yi and Wu, Wei and Mitragotri, Samir and Gan, Li and Qi, Jianping
Journal: ACS Applied Materials & Interfaces (2017)
Mesenchymal stem cells increase skin graft survival time and up-regulate PD-L1 expression in splenocytes of mice
Authors: Moravej, Ali and Geramizadeh, Bita and Azarpira, Negar and Zarnani, Amir-Hasan and Yaghobi, Ramin and Kalani, Mehdi and Khosravi, Maryam and Kouhpayeh, Amin and Karimi, Mohammad-Hossein
Journal: Immunology Letters (2017)
Authors: Moravej, Ali and Geramizadeh, Bita and Azarpira, Negar and Zarnani, Amir-Hasan and Yaghobi, Ramin and Kalani, Mehdi and Khosravi, Maryam and Kouhpayeh, Amin and Karimi, Mohammad-Hossein
Journal: Immunology Letters (2017)
Novel approach for the detection of intraperitoneal micrometastasis using an ovarian cancer mouse model
Authors: Alvero, Ayesha B and Kim, Dongin and Lima, Eydis and Sumi, Natalia J and Lee, Jung Seok and Cardenas, Carlos and Pitruzzello, Mary and Silasi, Dan-Arin and Buza, Natalia and Fahmy, Tarek and others, undefined
Journal: Scientific Reports (2017)
Authors: Alvero, Ayesha B and Kim, Dongin and Lima, Eydis and Sumi, Natalia J and Lee, Jung Seok and Cardenas, Carlos and Pitruzzello, Mary and Silasi, Dan-Arin and Buza, Natalia and Fahmy, Tarek and others, undefined
Journal: Scientific Reports (2017)
References
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A novel morphological technique to investigate a single climbing fibre synaptogenesis with a Purkinje cell in the developing mouse cerebellum: DiI injection into the inferior cerebellar peduncle
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Authors: Pavlidis M, Stupp T, Naskar R, Cengiz C, Thanos S.
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Authors: Levai O, Strotmann J.
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Application notes
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Evaluation of FLIPR Calcium Assays for Screening GPCR and Calcium Channel Targets
Introducing Calbryte™ Series
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Evaluation of FLIPR Calcium Assays for Screening GPCR and Calcium Channel Targets
Introducing Calbryte™ Series