DiD Dye
1,1-Dioctadecyl-3,3,3,3-tetramethylindodicarbocyanine; 5 mM DMSO solution
DiI, DiO, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures. The fluorescence of these environment-sensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins although they are weakly fluorescent in water. They have high extinction coefficients, polarity-dependent fluorescence and short excited-state lifetimes. Once applied to cells, these dyes diffuse laterally within the cellular plasma membranes, resulting in even staining of the entire cell at their optimal concentrations. The distinct fluorescence colors of DiI (orange fluorescence), DiO (green fluorescence), DiD (red fluorescence) and DiR (deep red fluorescent) provide a convenient tool for multicolor imaging and flow cytometric analysis of live cells. DiO and DiI can be used with standard FITC and TRITC filters respectively. Among them DiD is well excited by the 633 nm He-Ne laser, and has much longer excitation and emission wavelengths than those of DiI, providing a valuable alternative for labeling cells and tissues that have significant intrinsic fluorescence. DiR might be useful for in vivo imaging or tracing due to the effective transmission of infrared light through cells and tissues and low level of autofluorescence in the infrared range.
Spectrum
Citations
View all 62 citations: Citation Explorer
Observation of antitumor mechanism of GE11-modified paclitaxel and curcumin liposomes based on cellular morphology changes
Authors: Tang, Hailing and Li, Lijuan and Wang, Baoshan and Guangxi Scientific Research Center of Traditional Chinese Medicine,
Journal: AAPS Open (2024): 1
Authors: Tang, Hailing and Li, Lijuan and Wang, Baoshan and Guangxi Scientific Research Center of Traditional Chinese Medicine,
Journal: AAPS Open (2024): 1
An oncolytic virus--T cell chimera for cancer immunotherapy
Authors: Chen, Yuxuan and Chen, Xiaohong and Bao, Weier and Liu, Gang and Wei, Wei and Ping, Yuan
Journal: Nature Biotechnology (2024): 1--12
Authors: Chen, Yuxuan and Chen, Xiaohong and Bao, Weier and Liu, Gang and Wei, Wei and Ping, Yuan
Journal: Nature Biotechnology (2024): 1--12
Hyperglycemia-induced endothelial exosomes trigger trophoblast dysregulation and abnormal placentation through PUM2-mediated repression of SOX2
Authors: Zhu, Aibing and Qi, Suwan and Li, Wenjuan and Chen, Dashu and Zheng, Xiaomin and Xu, Jianjuan and Feng, Yaling
Journal: Human \& Experimental Toxicology (2023): 09603271221149656
Authors: Zhu, Aibing and Qi, Suwan and Li, Wenjuan and Chen, Dashu and Zheng, Xiaomin and Xu, Jianjuan and Feng, Yaling
Journal: Human \& Experimental Toxicology (2023): 09603271221149656
Enhanced osteoarthritis therapy by nanoengineered mesenchymal stem cells using biomimetic CuS nanoparticles loaded with plasmid DNA encoding TGF-$\beta$1
Authors: Cai, Yu and Wu, Cuixi and Ou, Qianhua and Zeng, Muhui and Xue, Song and Chen, Jieli and Lu, Yao and Ding, Changhai
Journal: Bioactive materials (2023): 444--457
Authors: Cai, Yu and Wu, Cuixi and Ou, Qianhua and Zeng, Muhui and Xue, Song and Chen, Jieli and Lu, Yao and Ding, Changhai
Journal: Bioactive materials (2023): 444--457
Ibuprofen in Therapeutic Concentrations Affects the Secretion of Human Bone Marrow Mesenchymal Stromal Cells, but Not Their Proliferative and Migratory Capacity
Authors: Kulesza, Agnieszka and Zielniok, Katarzyna and Hawryluk, Jakub and Paczek, Leszek and Burdzinska, Anna
Journal: Biomolecules (2022): 287
Authors: Kulesza, Agnieszka and Zielniok, Katarzyna and Hawryluk, Jakub and Paczek, Leszek and Burdzinska, Anna
Journal: Biomolecules (2022): 287
References
View all 92 references: Citation Explorer
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