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D-Luciferin, potassium salt *CAS#: 115144-35-9*
Luciferin is the most popular and versatile bioluminescent substrate. The firefly luciferase/luciferin bioluminescent system is found in the firefly (Photinus pyralis) and several other beetles. Luciferase oxidizes ATP-activated luciferin through a dioxetanone intermediate. Firefly luciferase produces light by the ATP-dependent oxidation of luciferin. The 560 nm chemiluminescence from this reaction peaks within seconds, with light output that is proportional to luciferase activity when luciferin and ATP are present in excess. Firefly luciferase has long been conjugated to antibodies and used as a label in immunoassays using luciferin as the substrate for detection. Compared to HRP and alkaline phosphatase, luciferase is less tolerant to chemical modifications. One particular advantage to the enzyme is that there is low endogenous luciferase activity in mammalian tissues besides its high sensitivity. Another important use of luciferase is in the area of hygiene monitoring. The luciferase/luciferin system can be used to detect contamination because ATP, present in all living organisms, is required to produce luminescence. The main application for this type of ATP bioluminescence is quality assurance by testing surfacesin food processing plants to determine whether or not there iscontamination of eitherequipment or products.
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Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of D-Luciferin, potassium salt *CAS#: 115144-35-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 314.06 µL | 1.57 mL | 3.141 mL | 15.703 mL | 31.406 mL |
| 5 mM | 62.812 µL | 314.06 µL | 628.121 µL | 3.141 mL | 6.281 mL |
| 10 mM | 31.406 µL | 157.03 µL | 314.06 µL | 1.57 mL | 3.141 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) |
| D-Luciferin, sodium salt *CAS#: 103404-75-7* | 385 | 529 |
Citations
View all 23 citations: Citation Explorer
Characterization of novel double-reporter strains of Mycobacterium abscessus for drug discovery: a study in mScarlet
Authors: Bento, Clara M and van Calster, Kevin and Piller, Tatiana and Oliveira, Gabriel S and de Vooght, Linda and Cappoen, Davie and Cos, Paul and Gomes, M Salom{\'e} and Silva, T{\^a}nia
Journal: Microbiology Spectrum (2024): e00362--24
Authors: Bento, Clara M and van Calster, Kevin and Piller, Tatiana and Oliveira, Gabriel S and de Vooght, Linda and Cappoen, Davie and Cos, Paul and Gomes, M Salom{\'e} and Silva, T{\^a}nia
Journal: Microbiology Spectrum (2024): e00362--24
Extracellular vesicle surface display of $\alpha$PD-L1 and $\alpha$CD3 antibodies via engineered late domain-based scaffold to activate T-cell anti-tumor immunity
Authors: Chen, Rui and Kang, Ziqin and Li, Wenhao and Xu, Tianshu and Wang, Yongqiang and Jiang, Qiming and Wang, Yuepeng and Huang, Zixian and Xu, Xiaoding and Huang, Zhiquan
Journal: Journal of Extracellular Vesicles (2024): e12490
Authors: Chen, Rui and Kang, Ziqin and Li, Wenhao and Xu, Tianshu and Wang, Yongqiang and Jiang, Qiming and Wang, Yuepeng and Huang, Zixian and Xu, Xiaoding and Huang, Zhiquan
Journal: Journal of Extracellular Vesicles (2024): e12490
Timely delivery of bone marrow mesenchymal stem cells based on the inflammatory pattern of bone injury environment to promote the repair of calvarial bone defects in rats: An optimized strategy for bone tissue engineering
Authors: Zeng, Yuwei and Lou, Aiju and Zhong, Zhenmin and Cai, Yu and Yang, Yixi and Liang, Haifeng and Lin, Yucong and He, Zhuoxuan and Zhou, Lei and Zhang, Zhi-Yong and others,
Journal: Journal of Tissue Engineering (2024): 20417314241252960
Authors: Zeng, Yuwei and Lou, Aiju and Zhong, Zhenmin and Cai, Yu and Yang, Yixi and Liang, Haifeng and Lin, Yucong and He, Zhuoxuan and Zhou, Lei and Zhang, Zhi-Yong and others,
Journal: Journal of Tissue Engineering (2024): 20417314241252960
Bmal1 integrates circadian function and temperature sensing in the suprachiasmatic nucleus
Authors: Hoekstra, Marieke MB and Ness, Natalie and Badia-Soteras, Aina and Brancaccio, Marco
Journal: Proceedings of the National Academy of Sciences (2024): e2316646121
Authors: Hoekstra, Marieke MB and Ness, Natalie and Badia-Soteras, Aina and Brancaccio, Marco
Journal: Proceedings of the National Academy of Sciences (2024): e2316646121
Water Quality Monitoring with the Multiplexed Assay MitoOxTox for Mitochondrial Toxicity, Oxidative Stress Response, and Cytotoxicity in AREc32 Cells
Authors: Lee, Jungeun and König, Maria and Braun, Georg and Escher, Beate I
Journal: Environmental Science \& Technology (2024)
Authors: Lee, Jungeun and König, Maria and Braun, Georg and Escher, Beate I
Journal: Environmental Science \& Technology (2024)
References
View all 113 references: Citation Explorer
Firefly luciferase produces hydrogen peroxide as a coproduct in dehydroluciferyl adenylate formation
Authors: Fraga H, Fern and es D, Novotny J, Fontes R, Esteves da Silva JC.
Journal: Chembiochem (2006): 929
Authors: Fraga H, Fern and es D, Novotny J, Fontes R, Esteves da Silva JC.
Journal: Chembiochem (2006): 929
cDNA cloning, expression and homology modeling of a luciferase from the firefly Lampyroidea maculata
Authors: Emamzadeh AR, Hosseinkhani S, Sadeghizadeh M, Nikkhah M, Chaichi MJ, Mortazavi M.
Journal: J Biochem Mol Biol (2006): 578
Authors: Emamzadeh AR, Hosseinkhani S, Sadeghizadeh M, Nikkhah M, Chaichi MJ, Mortazavi M.
Journal: J Biochem Mol Biol (2006): 578
Cloning and characterization of the homologous genes of firefly luciferase in the mealworm beetle, Tenebrio molitor
Authors: Oba Y, Sato M, Inouye S.
Journal: Insect Mol Biol (2006): 293
Authors: Oba Y, Sato M, Inouye S.
Journal: Insect Mol Biol (2006): 293
Structural basis for the spectral difference in luciferase bioluminescence
Authors: Nakatsu T, Ichiyama S, Hiratake J, Saldanha A, Kobashi N, Sakata K, Kato H.
Journal: Nature (2006): 372
Authors: Nakatsu T, Ichiyama S, Hiratake J, Saldanha A, Kobashi N, Sakata K, Kato H.
Journal: Nature (2006): 372
Evaluation of firefly luciferase bioluminescence mediated photodynamic toxicity in cancer cells
Authors: Schipper ML, Patel MR, Gambhir SS.
Journal: Mol Imaging Biol (2006): 218
Authors: Schipper ML, Patel MR, Gambhir SS.
Journal: Mol Imaging Biol (2006): 218
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