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D-Luciferin galactoside *CAS 131474-38-9*
D-Luciferin galactoside contains a beta-galactoside moiety attached at the 6-O-position, thus preventing it be recognized by the firefly luciferase enzyme. However, the galactose moiety is removed effectively by beta-galactosidase activity, and the resulted D-luciferin is well recognized by luciferase. D-Luciferin galactoside is a sensitive substrate for the chemiluminescent measurement of galactosidase activity in homogeneous assays, or in cell lysate samples in combination with luciferase and its cofactors. When used as a dual substrate, beta-galactosidase (LacZ gene) and luciferase levels can be determined with high sensitivity. When used in a model system expressing firefly luciferase, the luciferin is then utilized in a firefly luciferase reaction to generate light. It can also be used for in vivo bioluminescence imaging applications.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of D-Luciferin galactoside *CAS 131474-38-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 226.009 µL | 1.13 mL | 2.26 mL | 11.3 mL | 22.601 mL |
| 5 mM | 45.202 µL | 226.009 µL | 452.018 µL | 2.26 mL | 4.52 mL |
| 10 mM | 22.601 µL | 113.005 µL | 226.009 µL | 1.13 mL | 2.26 mL |
Molarity calculator
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| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) |
| D-Luciferin phosphate *CAS 145613-12-3* | 385 | 529 |
| D-Luciferin acetate | 385 | 529 |
Citations
View all 6 citations: Citation Explorer
Extracellular vesicle surface display of $\alpha$PD-L1 and $\alpha$CD3 antibodies via engineered late domain-based scaffold to activate T-cell anti-tumor immunity
Authors: Chen, Rui and Kang, Ziqin and Li, Wenhao and Xu, Tianshu and Wang, Yongqiang and Jiang, Qiming and Wang, Yuepeng and Huang, Zixian and Xu, Xiaoding and Huang, Zhiquan
Journal: Journal of Extracellular Vesicles (2024): e12490
Authors: Chen, Rui and Kang, Ziqin and Li, Wenhao and Xu, Tianshu and Wang, Yongqiang and Jiang, Qiming and Wang, Yuepeng and Huang, Zixian and Xu, Xiaoding and Huang, Zhiquan
Journal: Journal of Extracellular Vesicles (2024): e12490
Bmal1 integrates circadian function and temperature sensing in the suprachiasmatic nucleus
Authors: Hoekstra, Marieke MB and Ness, Natalie and Badia-Soteras, Aina and Brancaccio, Marco
Journal: Proceedings of the National Academy of Sciences (2024): e2316646121
Authors: Hoekstra, Marieke MB and Ness, Natalie and Badia-Soteras, Aina and Brancaccio, Marco
Journal: Proceedings of the National Academy of Sciences (2024): e2316646121
GRAS family member LATERAL SUPPRESSOR regulates the initiation and morphogenesis of watermelon lateral organs
Authors: Jiang, Yanxin and Zhang, Anran and He, Wenjing and Li, Qingqing and Zhao, Bosi and Zhao, Hongjiao and Ke, Xubo and Guo, Yalu and Sun, Piaoyun and Yang, Tongwen and others,
Journal: Plant Physiology (2023): kiad445
Authors: Jiang, Yanxin and Zhang, Anran and He, Wenjing and Li, Qingqing and Zhao, Bosi and Zhao, Hongjiao and Ke, Xubo and Guo, Yalu and Sun, Piaoyun and Yang, Tongwen and others,
Journal: Plant Physiology (2023): kiad445
Design, synthesis, and biological evaluation studies of novel carboxylesterase 2 inhibitors for the treatment of irinotecan-induced delayed diarrhea
Authors: Yang, Zhongcheng and Cao, Zhijun and Wang, Wenxin and Chen, Ya and Huang, Wanqiu and Jiao, Shixuan and Chen, Siliang and Chen, Lianru and Liu, Yuxia and Mao, Jianming and others,
Journal: Bioorganic Chemistry (2023): 106625
Authors: Yang, Zhongcheng and Cao, Zhijun and Wang, Wenxin and Chen, Ya and Huang, Wanqiu and Jiao, Shixuan and Chen, Siliang and Chen, Lianru and Liu, Yuxia and Mao, Jianming and others,
Journal: Bioorganic Chemistry (2023): 106625
Diltiazem inhibits breast cancer metastasis via mediating growth differentiation factor 15 and epithelial-mesenchymal transition
Authors: Chen, Yen-Chang and Wu, Chen-Teng and Chen, Jia-Hong and Tsai, Cheng-Fang and Wu, Chen-Yun and Chang, Pei-Chun and Yeh, Wei-Lan
Journal: Oncogenesis (2022): 1--9
Authors: Chen, Yen-Chang and Wu, Chen-Teng and Chen, Jia-Hong and Tsai, Cheng-Fang and Wu, Chen-Yun and Chang, Pei-Chun and Yeh, Wei-Lan
Journal: Oncogenesis (2022): 1--9
References
View all 3 references: Citation Explorer
Luminescent imaging of beta-galactosidase activity in living subjects using sequential reporter-enzyme luminescence
Authors: Wehrman TS, von Degenfeld G, Krutzik PO, Nolan GP, Blau HM.
Journal: Nat Methods (2006): 295
Authors: Wehrman TS, von Degenfeld G, Krutzik PO, Nolan GP, Blau HM.
Journal: Nat Methods (2006): 295
Homogeneous enzyme immunoassay modified for application to luminescence-based biosensors
Authors: Yang X, Janatova J, Andrade JD.
Journal: Anal Biochem (2005): 102
Authors: Yang X, Janatova J, Andrade JD.
Journal: Anal Biochem (2005): 102
Development of a rapid positive/absent test for coliforms using sensitive bioluminescence assay
Authors: Masuda-Nishimura I, Fukuda S, Sano A, Kasai K, Tatsumi H.
Journal: Lett Appl Microbiol (2000): 130
Authors: Masuda-Nishimura I, Fukuda S, Sano A, Kasai K, Tatsumi H.
Journal: Lett Appl Microbiol (2000): 130
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