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Cy3®-streptavidin conjugate
Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This Cy3-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with Cy3 covalently attached (as the fluorescent label). Our Cy3-streptavidin conjugate was prepared using AAT Bioquest's proprietary labeling technology. It demonstrated much brighter signal compared to the similar Cy3-streptavidin conjugates from other commercial sources, thus can significantly increase assay sensitivities. It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a valuable tool for biotin-streptavidin-based biological assays and tests using flow cytometry with a Cy3 filter set. A variety of the complementary biotinylated reagents are available from numerous commercial vendors.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| Cy3 biotin conjugate | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
| RPE-streptavidin conjugate | 565 | 574 | 1960000 | 0.82 | - | 0.175 |
| APC-streptavidin conjugate | 651 | 660 | 730000 | - | - | 0.195 |
| PerCP-streptavidin conjugate | 477 | 678 | 406000 | - | - | 0.22 |
| FITC-streptavidin conjugate | 491 | 516 | 73000 | 0.92 | - | 0.35 |
| Cy5®-streptavidin conjugate | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 |
| Cy7®-streptavidin conjugate | 756 | 779 | 250000 | 0.3 | 0.05 | 0.036 |
| XFD350-streptavidin conjugate *XFD350 Same Structure to Alexa Fluor™ 350* | 343 | 441 | 19000 | - | 0.25 | 0.19 |
| XFD488-streptavidin conjugate *XFD488 Same Structure to Alexa Fluor™ 488* | 499 | 520 | 73000 | 0.921 | 0.3 | 0.11 |
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Citations
View all 4 citations: Citation Explorer
Functional and Structural Development of Mouse Cone Photoreceptor Ribbon Synapses
Authors: Davison, Adam and Gierke, Kaspar and Brandst{\"a}tter, Johann Helmut and Babai, Norbert
Journal: Investigative Ophthalmology \& Visual Science (2022): 21--21
Authors: Davison, Adam and Gierke, Kaspar and Brandst{\"a}tter, Johann Helmut and Babai, Norbert
Journal: Investigative Ophthalmology \& Visual Science (2022): 21--21
Deciphering the Interactome of Histone Marks in Living Cells via Genetic Code Expansion Combined with Proximity Labeling
Authors: Huang, Yepei and Zhai, Guijin and Li, Yanan and Han, Yue and Chen, Chen and Lu, Congcong and Zhang, Kai
Journal: Analytical Chemistry (2022)
Authors: Huang, Yepei and Zhai, Guijin and Li, Yanan and Han, Yue and Chen, Chen and Lu, Congcong and Zhang, Kai
Journal: Analytical Chemistry (2022)
Alloantigen-activated (AAA) CD4+ T cells reinvigorate host endogenous T cell immunity to eliminate pre-established tumors in mice
Authors: Mochizuki, Kazuhiro and Kobayashi, Shogo and Takahashi, Nobuhisa and Sugimoto, Kotaro and Sano, Hideki and Ohara, Yoshihiro and Mineishi, Shin and Zhang, Yi and Kikuta, Atsushi
Journal: Journal of Experimental \& Clinical Cancer Research (2021): 1--18
Authors: Mochizuki, Kazuhiro and Kobayashi, Shogo and Takahashi, Nobuhisa and Sugimoto, Kotaro and Sano, Hideki and Ohara, Yoshihiro and Mineishi, Shin and Zhang, Yi and Kikuta, Atsushi
Journal: Journal of Experimental \& Clinical Cancer Research (2021): 1--18
Overexpression of MACC1 and the association with hepatocyte growth factor/c-Met in epithelial ovarian cancer
Authors: Li, Hongyu and Zhang, Hui and Zhao, Shujun and Shi, Yun and Yao, Junge and Zhang, Yanyan and Guo, Huanhuan and Liu, Xingsuo
Journal: Oncology letters (2015): 1989--1996
Authors: Li, Hongyu and Zhang, Hui and Zhao, Shujun and Shi, Yun and Yao, Junge and Zhang, Yanyan and Guo, Huanhuan and Liu, Xingsuo
Journal: Oncology letters (2015): 1989--1996
References
View all 47 references: Citation Explorer
A streptavidin paramagnetic-particle based competition assay for the evaluation of the optical selectivity of quadruplex nucleic acid fluorescent probes
Authors: Largy E, Hamon F, Teulade-Fichou MP.
Journal: Methods. (2012)
Authors: Largy E, Hamon F, Teulade-Fichou MP.
Journal: Methods. (2012)
Biotin-4-fluorescein based fluorescence quenching assay for determination of biotin binding capacity of streptavidin conjugated quantum dots
Authors: Mittal R, Bruchez MP.
Journal: Bioconjug Chem (2011): 362
Authors: Mittal R, Bruchez MP.
Journal: Bioconjug Chem (2011): 362
Iminobiotin binding induces large fluorescent enhancements in avidin and streptavidin fluorescent conjugates and exhibits diverging pH-dependent binding affinities
Authors: Raphael MP, Rappole CA, Kurihara LK, Christodoulides JA, Qadri SN, Byers JM.
Journal: J Fluoresc (2011): 647
Authors: Raphael MP, Rappole CA, Kurihara LK, Christodoulides JA, Qadri SN, Byers JM.
Journal: J Fluoresc (2011): 647
Streptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complex
Authors: Kim JH, Chang TM, Graham AN, Choo KH, Kalitsis P, Hudson DF.
Journal: BMC Biochem (2010): 50
Authors: Kim JH, Chang TM, Graham AN, Choo KH, Kalitsis P, Hudson DF.
Journal: BMC Biochem (2010): 50
Multimodality nuclear and fluorescence tumor imaging in mice using a streptavidin nanoparticle
Authors: Liang M, Liu X, Cheng D, Liu G, Dou S, Wang Y, Rusckowski M, Hnatowich DJ.
Journal: Bioconjug Chem (2010): 1385
Authors: Liang M, Liu X, Cheng D, Liu G, Dou S, Wang Y, Rusckowski M, Hnatowich DJ.
Journal: Bioconjug Chem (2010): 1385
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