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PerCP-streptavidin conjugate

Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This PerCP-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with PerCP covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a very valuable tool for biotin-streptavidin-based biological assays and tests using flow cytometry platform. A variety of the complementary biotinylated reagents are available from numerous commercial vendors.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (280 nm)
APC-streptavidin conjugate651660730000-0.195
Cy3®-streptavidin conjugate55556915000010.1510.073
Cy5®-streptavidin conjugate65167025000010.271, 0.420.03
Cy7®-streptavidin conjugate7567792500000.30.036
FITC-streptavidin conjugate491516730000.920.35
RPE-streptavidin conjugate56557419600000.820.175

Citations

View all 1 citations: Citation Explorer
Overexpression of MACC1 and the association with hepatocyte growth factor/c-Met in epithelial ovarian cancer
Authors: Li, Hongyu and Zhang, Hui and Zhao, Shujun and Shi, Yun and Yao, Junge and Zhang, Yanyan and Guo, Huanhuan and Liu, Xingsuo
Journal: Oncology letters (2015): 1989--1996

References

View all 47 references: Citation Explorer
A streptavidin paramagnetic-particle based competition assay for the evaluation of the optical selectivity of quadruplex nucleic acid fluorescent probes
Authors: Largy E, Hamon F, Teulade-Fichou MP.
Journal: Methods. (2012)
Biotin-4-fluorescein based fluorescence quenching assay for determination of biotin binding capacity of streptavidin conjugated quantum dots
Authors: Mittal R, Bruchez MP.
Journal: Bioconjug Chem (2011): 362
Iminobiotin binding induces large fluorescent enhancements in avidin and streptavidin fluorescent conjugates and exhibits diverging pH-dependent binding affinities
Authors: Raphael MP, Rappole CA, Kurihara LK, Christodoulides JA, Qadri SN, Byers JM.
Journal: J Fluoresc (2011): 647
Streptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complex
Authors: Kim JH, Chang TM, Graham AN, Choo KH, Kalitsis P, Hudson DF.
Journal: BMC Biochem (2010): 50
Determination of 17beta-oestradiol by fluorescence immunoassay with streptavidin-conjugated quantum dots as label
Authors: Sun M, Du L, Gao S, Bao Y, Wang S.
Journal: Steroids (2010): 400
Page updated on April 15, 2025

Ordering information

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Unit size
Catalog Number16905
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Telephone1-800-990-8053
Fax1-800-609-2943
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Physical properties

Molecular weight

~87000

Solvent

Water

Spectral properties

Correction Factor (280 nm)

0.22

Extinction coefficient (cm -1 M -1)

406000

Excitation (nm)

477

Emission (nm)

678

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Refrigerated (2-8 °C); Minimize light exposure
UNSPSC12352200
Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of whole blood cells stained first with CD4–biotin followed by PerCP–streptavidin conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PerCP–specific B8-A channel.
Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of whole blood cells stained first with CD4–biotin followed by PerCP–streptavidin conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PerCP–specific B8-A channel.
Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of whole blood cells stained first with CD4–biotin followed by PerCP–streptavidin conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PerCP–specific B8-A channel.
HL-60 cells were incubated with (Red, +) or without (Green, -) mouse Anti-Human HLA-ABC Biotin (W6/32-Biotin) followed by PerCP-streptavidin conjugate. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in PerCP channel.

Documents

pdfSafety data sheet
pdfProduct protocol
pdfCertificate of analysis
Biotin and Streptavidin
APC-iFluor® 750-streptavidin conjugate
iFluor® 350-streptavidin conjugate
iFluor® 405-streptavidin conjugate