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Custom Labeling
AAT Bioquest offers same-day custom conjugation of antibodies or proteins with a wide array of labels, such as biotin, HRP and over 20 fluorophores. Our service is designed for high-quality results with fast turnaround, to supply scientists with flexible tools that help expand their research.
You may supply your own antibody or chose from 3000+ monoclonal and polyclonal antibodies in our catalog. Contact us for a quote today, and let us put our expertise to work for you!
How does it work?
Antibody conjugates allow for the study of specific antigens in biological systems. Such targets may include proteins (like polymerase), small molecules (like cAMP) or nucleic acids (like dsDNA). Conjugates can be made with both primary and secondary antibodies. Primary, or direct, immunofluorescence utilizes a single antibody which both detects the antigen and emits the signal. Secondary, or indirect, immunofluorescence uses two antibodies, one which recognizes the target antigen and a secondary antibody which binds to the primary antibody and emits the signal.
Antibodies can be conjugated with reporter enzymes like horseradish peroxidase (HRP) for use in assays such as Western Blot, ELISA and immunohistochemistry. Depending on the substrate it is paired with, such conjugates can yield signals that are colorimetric or fluorometric in nature. Additionally, different substrates will produce signals with different spectral properties. Some common substrates are TMB and Amplite Red, which yield signals at 650 nm and 585 nm, respectively.
Antibodies can also be conjugated with a variety of fluorochromes for use in applications such as flow cytometry or fluorescence microscopy. These are labels which will emit a detectable array of photons when excited. Possible fluorochromes span the spectrum from UV to Near Infrared. Common fluorochromes include markers such as FITC and Texas Red®.
Picking the right label
Picking the correct label to use for your experiment can be difficult, especially when there are so many choices. Here are some things to keep in mind.
Picking the correct label can be challenging. Let our experts help simplify the process! Contact us for a consultation and let us work with you to pick the best label to suit your specific needs.
Available Labels
Please contact us at info@aatbio.com to inquire about additional or custom labels.
Gallery
Additional Services
In addition to custom conjugation, we offer antibody development services for monoclonal and polyclonal antibodies. For a complete list of other services we offer, please click here. Alternatively, you can contact us directly at info@aatbio.com for project consultations.
*Availablility of 24-hour conjungation service depends on specific antibody/label pair. Call for more information.
Guaranteed quality with 95% purity rating
Affordable price tiers that work with your budget
Scalable service with minimum order of 100 µg
Same day order fulfilment means your conjugate ships the day you order it*
You may supply your own antibody or chose from 3000+ monoclonal and polyclonal antibodies in our catalog. Contact us for a quote today, and let us put our expertise to work for you!
How does it work?
Antibody conjugates allow for the study of specific antigens in biological systems. Such targets may include proteins (like polymerase), small molecules (like cAMP) or nucleic acids (like dsDNA). Conjugates can be made with both primary and secondary antibodies. Primary, or direct, immunofluorescence utilizes a single antibody which both detects the antigen and emits the signal. Secondary, or indirect, immunofluorescence uses two antibodies, one which recognizes the target antigen and a secondary antibody which binds to the primary antibody and emits the signal.
Antibodies can be conjugated with reporter enzymes like horseradish peroxidase (HRP) for use in assays such as Western Blot, ELISA and immunohistochemistry. Depending on the substrate it is paired with, such conjugates can yield signals that are colorimetric or fluorometric in nature. Additionally, different substrates will produce signals with different spectral properties. Some common substrates are TMB and Amplite Red, which yield signals at 650 nm and 585 nm, respectively.
Antibodies can also be conjugated with a variety of fluorochromes for use in applications such as flow cytometry or fluorescence microscopy. These are labels which will emit a detectable array of photons when excited. Possible fluorochromes span the spectrum from UV to Near Infrared. Common fluorochromes include markers such as FITC and Texas Red®.
Picking the right label
Picking the correct label to use for your experiment can be difficult, especially when there are so many choices. Here are some things to keep in mind.
| Consideration | Explanation | Our Suggestion |
| Single or Multiplex Format? | If you are running a multiplex experiment, you need to be aware of spectral overlaps. Too much overlap will lead to signal bleed-through between fluorophores. | Use our Spectrum Viewer web application to compare fluorophore excitation/emission and minimize overlaps. |
| Instrumentation | Make sure that your instrument can properly detect the chosen fluorophore. Is it colorimetric or fluorimetric? Does it have the proper excitation sources? Does it have the correct filters? | Check our fluorophore product page for specific spectral properties to see if it is compatible with your instrumentation. |
| Experimental Conditions | Does your experiment require special conditions such as temperature or pH? Many fluorophores are sensitive to experimental conditions, which will impact their brightness and susceptibility to photo-bleaching. | Check our fluorophore product page for specific restrictions on usage. |
Picking the correct label can be challenging. Let our experts help simplify the process! Contact us for a consultation and let us work with you to pick the best label to suit your specific needs.
Available Labels
| Proteins | HRP, Alkaline Phosphatase, Streptavidin |
| Small Molecules | Biotin |
| Tandems | APC, RPE |
| Fluorophore | Excitation (nm) | Emission (nm) | |
| iFluor 405 | 401 | 420 | |
| iFluor 350 | 346 | 443 | |
| FITC | 490 | 514 | |
| AF 488 | 494 | 517 | |
| iFluor 488 | 491 | 518 | |
| iFluor 555 | 555 | 565 | |
| Cy3 | 555 | 565 | |
| TRITC | 543 | 571 | |
| Rhodamine B | 552 | 588 | |
| iFluor 594 | 594 | 614 | |
| AF 594 | 590 | 617 | |
| iFluor 633 | 638 | 655 | |
| iFluor 647 | 649 | 664 | |
| Cy5 | 649 | 665 | |
| Cy5.5 | 678 | 701 | |
| iFluor 700 | 685 | 710 | |
| Cy7 | 749 | 776 | |
| iFluor 790 | 782 | 811 |
Please contact us at info@aatbio.com to inquire about additional or custom labels.
Gallery
Additional Services
In addition to custom conjugation, we offer antibody development services for monoclonal and polyclonal antibodies. For a complete list of other services we offer, please click here. Alternatively, you can contact us directly at info@aatbio.com for project consultations.
*Availablility of 24-hour conjungation service depends on specific antibody/label pair. Call for more information.