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5(6)-TRITC [Tetramethylrhodamine-5-(and-6)-isothiocyanate] *CAS 95197-95-8*
5(6)-TRITC is an amino-reactive labeling reagent that is widely used in preparing bioconjugates of proteins and nucleic acids. The resultant conjugates have similar spectral properties to those prepared from 5(6)-TAMRA, SE. However, the latter conjugates are much more stable. Cautions must be exercised for the storage of TRITC conjugates.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 5(6)-TRITC [Tetramethylrhodamine-5-(and-6)-isothiocyanate] *CAS 95197-95-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 208.342 µL | 1.042 mL | 2.083 mL | 10.417 mL | 20.834 mL |
| 5 mM | 41.668 µL | 208.342 µL | 416.684 µL | 2.083 mL | 4.167 mL |
| 10 mM | 20.834 µL | 104.171 µL | 208.342 µL | 1.042 mL | 2.083 mL |
Molarity calculator
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| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
| 5(6)-CR6G [5-(and 6)-Carboxyrhodamine 6G] | 522 | 546 | 94000 | 0.24 | 0.214 |
| 5(6)-TAMRA [5(6)-Carboxytetramethylrhodamine] *CAS 98181-63-6* | 552 | 578 | 90000 | 0.32 | 0.178 |
| 5(6)-ROX [5-(and 6)-Carboxy-X-rhodamine] *CAS 198978-94-8* | 578 | 604 | 82000 | - | 0.168 |
| 5(6)-FAM [5-(and-6)-Carboxyfluorescein] *CAS 72088-94-9* | 493 | 517 | 83000 | 0.32 | 0.178 |
References
View all 45 references: Citation Explorer
Recombinant human erythropoietin prevents ischemia-induced apoptosis and necrosis in explant cultures of the rat organ of Corti
Authors: Andreeva N, Nyamaa A, Haupt H, Gross J, Mazurek B.
Journal: Neurosci Lett (2006): 86
Authors: Andreeva N, Nyamaa A, Haupt H, Gross J, Mazurek B.
Journal: Neurosci Lett (2006): 86
Mechanism of advanced glycation end products-induced hyperpermeability in endothelial cells.
Authors: Guo XH, Huang QB, Chen B, Wang SY, Hou FF, Fu N.
Journal: Sheng Li Xue Bao (2005): 205
Authors: Guo XH, Huang QB, Chen B, Wang SY, Hou FF, Fu N.
Journal: Sheng Li Xue Bao (2005): 205
Musculature of an illoricate predatory rotifer Asplanchnopus multiceps as revealed by phalloidin fluorescence and confocal microscopy
Authors: Kotikova EA, Raikova OI, Reuter M, Gustafsson MK.
Journal: Tissue Cell (2004): 189
Authors: Kotikova EA, Raikova OI, Reuter M, Gustafsson MK.
Journal: Tissue Cell (2004): 189
Resonance energy transfer nanobiosensors based on affinity binding between apo-enzyme and its substrate
Authors: Chinnayelka S, McShane MJ.
Journal: Biomacromolecules (2004): 1657
Authors: Chinnayelka S, McShane MJ.
Journal: Biomacromolecules (2004): 1657
Nontypeable Haemophilus influenzae strain 2019 produces a biofilm containing N-acetylneuraminic acid that may mimic sialylated O-linked glycans
Authors: Greiner LL, Watanabe H, Phillips NJ, Shao J, Morgan A, Zaleski A, Gibson BW, Apicella MA.
Journal: Infect Immun (2004): 4249
Authors: Greiner LL, Watanabe H, Phillips NJ, Shao J, Morgan A, Zaleski A, Gibson BW, Apicella MA.
Journal: Infect Immun (2004): 4249
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