Rhodamine B *CAS 81-88-9*
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 479.01 |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 541 |
Extinction coefficient (cm -1 M -1) | 1060001 |
Excitation (nm) | 546 |
Emission (nm) | 567 |
Quantum yield | 0.71, 0.432 |
Storage, safety and handling
Certificate of Origin | Download PDF |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
Overview | ![]() ![]() |
See also: Rhodamines and Rhodamine Derivatives
CAS 81-88-9 | Molecular weight 479.01 | Absorbance (nm) 541 | Extinction coefficient (cm -1 M -1) 1060001 | Excitation (nm) 546 | Emission (nm) 567 | Quantum yield 0.71, 0.432 |
Rhodamine B (also called Rhodamine 610, Basic Violet 10, or C.I. 45170) is used as a fluorescent staining dye, sometimes in combination with auramine O. The auramine-rhodamine stain is used to demonstrate acid-fast organisms, notably Mycobacterium; Rhodamine B is tunable around 610 nm when used as a laser dye.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Rhodamine B *CAS 81-88-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 208.764 µL | 1.044 mL | 2.088 mL | 10.438 mL | 20.876 mL |
5 mM | 41.753 µL | 208.764 µL | 417.528 µL | 2.088 mL | 4.175 mL |
10 mM | 20.876 µL | 104.382 µL | 208.764 µL | 1.044 mL | 2.088 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Spectrum
Open in Advanced Spectrum Viewer
![spectrum](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fspectra%2Frhodamine_b.png&w=2048&q=50)
![spectrum](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fspectra%2Frhodamine_b.png&w=2048&q=50)
Spectral properties
Absorbance (nm) | 541 |
Extinction coefficient (cm -1 M -1) | 1060001 |
Excitation (nm) | 546 |
Emission (nm) | 567 |
Quantum yield | 0.71, 0.432 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
Rhodamine 800 *CAS 137993-41-0* | 682 | 704 | - |
Rhodamine 6G *CAS 989-38-8* | 525 | 548 | 1160001 |
Sulforhodamine B *CAS 3520-42-1* | 559 | 577 | - |
Rhodamine 110 *CAS 13558-31-1* | 500 | 522 | 80000 |
Rhodamine 123 *CAS 62669-70-9* | 508 | 528 | 85200 |
Rhodamine aldehyde [5-TAMRA aldehyde] | 552 | 578 | 90000 |
Citations
View all 1 citations: Citation Explorer
Optothermal crystallization of hard spheres in an effective bidimensional geometry
Authors: Ruzzi, Vincenzo and Baglioni, Jacopo and Piazza, Roberto
Journal: The Journal of Chemical Physics (2023)
Authors: Ruzzi, Vincenzo and Baglioni, Jacopo and Piazza, Roberto
Journal: The Journal of Chemical Physics (2023)
References
View all 39 references: Citation Explorer
Adsorption behavior of rhodamine B on Rhizopus oryzae biomass
Authors: Das SK, Bhowal J, Das AR, Guha AK.
Journal: Langmuir (2006): 7265
Authors: Das SK, Bhowal J, Das AR, Guha AK.
Journal: Langmuir (2006): 7265
Controllable size, shape and morphology of molybdic acid self-aggregated with rhodamine B to construct functional material
Authors: Guan ZS, Zhang Y, Zhang Q, Li DX.
Journal: J Colloid Interface Sci (2006): 113
Authors: Guan ZS, Zhang Y, Zhang Q, Li DX.
Journal: J Colloid Interface Sci (2006): 113
Interaction of a mitochondrial membrane potential-sensitive dye, rhodamine 800, with rat mitochondria, cells, and perfused hearts
Authors: Jilkina O, Kong HJ, Hwi L, Kuzio B, Xiang B, Manley D, Jackson M, Kupriyanov VV.
Journal: J Biomed Opt (2006): 14009
Authors: Jilkina O, Kong HJ, Hwi L, Kuzio B, Xiang B, Manley D, Jackson M, Kupriyanov VV.
Journal: J Biomed Opt (2006): 14009
Relationship between excretion clearance of rhodamine 123 and P-glycoprotein (Pgp) expression induced by representative Pgp inducers
Authors: Kageyama M, Fukushima K, Togawa T, Fujimoto K, Taki M, Nishimura A, Ito Y, Sugioka N, Shibata N, Takada K.
Journal: Biol Pharm Bull (2006): 779
Authors: Kageyama M, Fukushima K, Togawa T, Fujimoto K, Taki M, Nishimura A, Ito Y, Sugioka N, Shibata N, Takada K.
Journal: Biol Pharm Bull (2006): 779
Fluorescent plasma nanocomposite thin films containing nonaggregated rhodamine 6G laser dye molecules
Authors: Barranco A, Groening P.
Journal: Langmuir (2006): 6719
Authors: Barranco A, Groening P.
Journal: Langmuir (2006): 6719
Fluorescence resonance energy transfer quenching method for determination of arsenic with acridine orange-rhodamine B
Authors: Liu BS, Gao J, Liu ZC, Yu LN, Yang DC, Yang GL.
Journal: Guang Pu Xue Yu Guang Pu Fen Xi (2006): 306
Authors: Liu BS, Gao J, Liu ZC, Yu LN, Yang DC, Yang GL.
Journal: Guang Pu Xue Yu Guang Pu Fen Xi (2006): 306
Resonance scattering effect of rhodamine dye association nanoparticles and its application to respective determination of trace ClO2 and Cl2
Authors: Jiang ZL, Zhou SM, Liang AH, Kang C, He X.
Journal: Environ Sci Technol (2006): 4286
Authors: Jiang ZL, Zhou SM, Liang AH, Kang C, He X.
Journal: Environ Sci Technol (2006): 4286
Complex formation between rhodamine B and poly(sodium 4-styrenesulfonate) studied by 1H-NMR
Authors: Moreno-Villoslada I, Gonzalez R, Hess S, Rivas BL, Shibue T, Nishide H.
Journal: J Phys Chem B Condens Matter Mater Surf Interfaces Biophys (2006): 21576
Authors: Moreno-Villoslada I, Gonzalez R, Hess S, Rivas BL, Shibue T, Nishide H.
Journal: J Phys Chem B Condens Matter Mater Surf Interfaces Biophys (2006): 21576
Ab initio study of optical properties of rhodamine 6G molecular dimers
Authors: Gavrilenko VI, Noginov MA.
Journal: J Chem Phys (2006): 44301
Authors: Gavrilenko VI, Noginov MA.
Journal: J Chem Phys (2006): 44301
Determination of trace lead by solid substrate room temperature phosphorescence enhancing method based on heavy atom effect and dissoluble manganese supramolecule containing rhodamine 6G luminescent particles
Authors: Lin X, Wu RH, Li XJ, Chen L, Lin QW, Gao WY, Zheng AF, Liu JM, Zhu GH, Huang XM.
Journal: Spectrochim Acta A Mol Biomol Spectrosc. (2006)
Authors: Lin X, Wu RH, Li XJ, Chen L, Lin QW, Gao WY, Zheng AF, Liu JM, Zhu GH, Huang XM.
Journal: Spectrochim Acta A Mol Biomol Spectrosc. (2006)
Application notes
A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets