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Rhodamine 110 *CAS 13558-31-1*

Rhodamine 110 (R110) is the precursor for a variety of R110-based enzyme substrates. It is also used as a calibration standard for R110-based enzyme substrates. Compared to AMC and AFC enzyme substrates, fluorogenic R110 substrates are generally more sensitive, giving longer absorption and fluorescence wavelength than AMC and AFC substrates.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Rhodamine 110 *CAS 13558-31-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM272.628 µL1.363 mL2.726 mL13.631 mL27.263 mL
5 mM54.526 µL272.628 µL545.256 µL2.726 mL5.453 mL
10 mM27.263 µL136.314 µL272.628 µL1.363 mL2.726 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
Rhodamine 800 *CAS 137993-41-0*682704-
Rhodamine B *CAS 81-88-9*5465671060001
Rhodamine 6G *CAS 989-38-8*5255481160001
Rhodamine 123 *CAS 62669-70-9*50852885200
Rhodamine aldehyde [5-TAMRA aldehyde]55257890000

Citations

View all 1 citations: Citation Explorer
Phosphatase-degradable Nanoparticles: A game-changing approach for the delivery of antifungal proteins
Authors: Akku{\c{s}}-Da{\u{g}}deviren, Zeynep Burcu and Saleh, Ahmad and Sch{\"o}pf, Cristina and Truszkowska, Martyna and Bratschun-Khan, Doris and F{\"u}rst, Andrea and Seybold, Anna and Offterdinger, Martin and Marx, Florentine and Bernkop-Schn{\"u}rch, Andreas
Journal: Journal of Colloid and Interface Science (2023)

References

View all 8 references: Citation Explorer
N-DEVD-N'-morpholinecarbonyl-rhodamine 110: novel caspase-3 fluorogenic substrates for cell-based apoptosis assay
Authors: Wang ZQ, Liao J, Diwu Z.
Journal: Bioorg Med Chem Lett (2005): 2335
Fluorogenic substrate [Ala-Pro]2-cresyl violet but not Ala-Pro-rhodamine 110 is cleaved specifically by DPPIV activity: a study in living Jurkat cells and CD26/DPPIV-transfected Jurkat cells
Authors: Boonacker E, Elferink S, Bardai A, Fleischer B, Van Noorden CJ.
Journal: J Histochem Cytochem (2003): 959
A transgenic mouse model with a luciferase reporter for studying in vivo transcriptional regulation of the human CYP3A4 gene
Authors: Zhang W, Purchio AF, Chen K, Wu J, Lu L, Coffee R, Contag PR, West DB.
Journal: Drug Metab Dispos (2003): 1054
Design and synthesis of rhodamine 110 derivative and caspase-3 substrate for enzyme and cell-based fluorescent assay
Authors: Cai SX, Zhang HZ, Guastella J, Drewe J, Yang W, Weber E.
Journal: Bioorg Med Chem Lett (2001): 39
Rhodamine 110-linked amino acids and peptides as substrates to measure caspase activity upon apoptosis induction in intact cells
Authors: Hug H, Los M, Hirt W, Debatin KM.
Journal: Biochemistry (1999): 13906
Page updated on November 21, 2024

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Physical properties

Molecular weight

366.8

Solvent

DMSO

Spectral properties

Extinction coefficient (cm -1 M -1)

80000

Excitation (nm)

500

Emission (nm)

522

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

13558-31-1