Biotin-dT Phosphoramidite
Biotin-dT phosphoramidite contains a DMT group to allow quantification of coupling. It can be inserted into a target sequence as a replacement for a dT residue. It provides a valuable tool to add the biotin tag to a desired location of an oligo sequence. It is complimentary to the commonly used biotin phosphoramidite that contains no DMT group and can only be added at the 5'-terminus, thus terminating synthesis. The DMT group on biotin-dT phosphoramidite can be used in RP cartridge and HPLC purification techniques. AAT Bioquest also offer FAM-dT phosphoramidite, HEX-dT phosphoramidite, TET-dT phosphoramidite, biotin-dT CPG and Fluorescein-dT CPG.
References
View all 1 references: Citation Explorer
Photocleavable biotin phosphoramidite for 5'-end-labeling, affinity purification and phosphorylation of synthetic oligonucleotides.
Authors: Olejnik, J and Krzymańska-Olejnik, E and Rothschild, K J
Journal: Nucleic acids research (1996): 361-6
Authors: Olejnik, J and Krzymańska-Olejnik, E and Rothschild, K J
Journal: Nucleic acids research (1996): 361-6
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