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Bio-16-UTP [Biotin-16-UTP] *1 mM*
Bio-16-UTP is biotinylated UTP that can be used as a substrate in in vitro transcription reactions with a variety of RNA polymerases, including T7 RNA polymerase, SP6 RNA polymerase, or T3 RNA polymerase. Biotinylated RNA probes may be used in place of radioactively labeled RNA probes in many applications, including in situ hybridization and chromosome mapping. Bio-16-UTP can be detected using a variety of methods based on streptavidin binding. Biotin-bound streptavidin can be detected directly, using fluorescently labeled streptavidin, or indirectly, using a streptavidin-conjugated enzyme assay or appropriately labeled or conjugated anti-streptavidin antibody.
References
View all 8 references: Citation Explorer
Electrochemical biosensor for microRNA detection based on poly(U) polymerase mediated isothermal signal amplification.
Authors: Zhou, Yunlei and Yin, Huanshun and Li, Jie and Li, Bingchen and Li, Xue and Ai, Shiyun and Zhang, Xiansheng
Journal: Biosensors & bioelectronics (2016): 79-85
Authors: Zhou, Yunlei and Yin, Huanshun and Li, Jie and Li, Bingchen and Li, Xue and Ai, Shiyun and Zhang, Xiansheng
Journal: Biosensors & bioelectronics (2016): 79-85
Isolation of translating ribosomes containing peptidyl-tRNAs for functional and structural analyses.
Authors: Shirole, Nitin and Balasubramanian, Sreeram and Yanofsky, Charles and Cruz-Vera, Luis
Journal: Journal of visualized experiments : JoVE (2011)
Authors: Shirole, Nitin and Balasubramanian, Sreeram and Yanofsky, Charles and Cruz-Vera, Luis
Journal: Journal of visualized experiments : JoVE (2011)
Duplex in situ hybridization in the study of gene co-regulation in the vertebrate brain.
Authors: Pinaud, Raphael and Jeong, Jin K
Journal: Methods in molecular biology (Clifton, N.J.) (2010): 115-29
Authors: Pinaud, Raphael and Jeong, Jin K
Journal: Methods in molecular biology (Clifton, N.J.) (2010): 115-29
rRNA probe-based cell fishing of bacteria.
Authors: Stoffels, M and Ludwig, W and Schleifer, K H
Journal: Environmental microbiology (1999): 259-71
Authors: Stoffels, M and Ludwig, W and Schleifer, K H
Journal: Environmental microbiology (1999): 259-71
An in situ hybridization histochemistry technique allowing simultaneous visualization by the use of confocal microscopy of three cellular mRNA species in individual neurons.
Authors: Grino, M and Zamora, A J
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (1998): 753-9
Authors: Grino, M and Zamora, A J
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (1998): 753-9
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