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ATTO 565 maleimide
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 733.17 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (260 nm) | 0.34 |
| Correction Factor (280 nm) | 0.16 |
| Extinction coefficient (cm -1 M -1) | 120000 |
| Excitation (nm) | 563 |
| Emission (nm) | 589 |
| Quantum yield | 0.90 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
| ATTO 565 acid |
| Overview |  SDSProtocol |
Molecular weight 733.17 | Correction Factor (260 nm) 0.34 | Correction Factor (280 nm) 0.16 | Extinction coefficient (cm -1 M -1) 120000 | Excitation (nm) 563 | Emission (nm) 589 | Quantum yield 0.90 |
ATTO 565 Maleimide is a commonly used thiol-reactive rhodamine dye. It might be used for labeling peptides, proteins and other thiol-containing compounds such as thiol-modified oligos. It readily reacts with compounds containing thiol groups, forming a chemically stable thiol ether bond between the dye and thiol compounds. The optimum pH range for maleimide coupling is pH 5.5 – 7.0. ATTO 565 Maleimide has strong absorption, high fluorescence quantum yield and high thermal/photostability. However, it has low water solubility. For the applications that require high water solubility, you may consider iFluor® 568 Maleimide that has almost identical fluorescence properties with greatly enhanced water solubility. In addition, the protein conjugates made from iFluor® 568 Maleimide are much brighter than those from ATTO 565 Maleimide.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of ATTO 565 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 136.394 µL | 681.97 µL | 1.364 mL | 6.82 mL | 13.639 mL |
| 5 mM | 27.279 µL | 136.394 µL | 272.788 µL | 1.364 mL | 2.728 mL |
| 10 mM | 13.639 µL | 68.197 µL | 136.394 µL | 681.97 µL | 1.364 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (260 nm) | 0.34 |
| Correction Factor (280 nm) | 0.16 |
| Extinction coefficient (cm -1 M -1) | 120000 |
| Excitation (nm) | 563 |
| Emission (nm) | 589 |
| Quantum yield | 0.90 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| ATTO 488 maleimide | 499 | 520 | 90000 | 0.80 | 0.25 | 0.10 |
| ATTO 532 maleimide | 531 | 552 | 115000 | 0.90 | 0.22 | 0.11 |
| ATTO 647 maleimide | 646 | 666 | 120000 | 0.20 | 0.08 | 0.04 |
| ATTO 647N maleimide | 645 | 663 | 150000 | 0.651 | 0.06 | 0.05 |
| ATTO 594 maleimide | 602 | 621 | 120000 | 0.85 | 0.26 | 0.51 |
| ATTO 514 maleimide | 510 | 531 | 115,000 | 0.85 | 0.21 | 0.08 |
Images
Figure 1. ATTO 565 acid is a commonly used thiol-reactive rhodamine dye.
References
View all 32 references: Citation Explorer
Time-resolved fluorescence anisotropy with Atto 488-labeled phytochrome Agp1 from Agrobacterium fabrum.
Authors: Elkurdi, Afaf and Guigas, Gernot and Hourani-Alsharafat, Latifa and Scheerer, Patrick and Nienhaus, Gerd Ulrich and Krauß, Norbert and Lamparter, Tilman
Journal: Photochemistry and photobiology (2024): 561-572
Authors: Elkurdi, Afaf and Guigas, Gernot and Hourani-Alsharafat, Latifa and Scheerer, Patrick and Nienhaus, Gerd Ulrich and Krauß, Norbert and Lamparter, Tilman
Journal: Photochemistry and photobiology (2024): 561-572
Analysis of photon trajectories from diffusing single molecules.
Authors: Gopich, Irina V and Kim, Jae-Yeol and Chung, Hoi Sung
Journal: The Journal of chemical physics (2023)
Authors: Gopich, Irina V and Kim, Jae-Yeol and Chung, Hoi Sung
Journal: The Journal of chemical physics (2023)
Curvature preference of cubic CsPbBr3 quantum dots embedded onto phospholipid bilayer membranes.
Authors: Chairil, Ricki and Malmstadt, Noah
Journal: Soft matter (2023): 3966-3974
Authors: Chairil, Ricki and Malmstadt, Noah
Journal: Soft matter (2023): 3966-3974
Interactions of the Kv1.1 Channel with Peptide Pore Blockers: A Fluorescent Analysis on Mammalian Cells.
Authors: Orlov, Nikita A and Kryukova, Elena V and Efremenko, Anastasia V and Yakimov, Sergey A and Toporova, Victoria A and Kirpichnikov, Mikhail P and Nekrasova, Oksana V and Feofanov, Alexey V
Journal: Membranes (2023)
Authors: Orlov, Nikita A and Kryukova, Elena V and Efremenko, Anastasia V and Yakimov, Sergey A and Toporova, Victoria A and Kirpichnikov, Mikhail P and Nekrasova, Oksana V and Feofanov, Alexey V
Journal: Membranes (2023)
Spatial distribution of single guest molecules along thickness of thin films of poly(2-hydroxyethyl acrylate).
Authors: Ito, Syoji and Hiratsuka, Kengo and Takei, Satoshi and Nishi, Hiroyasu and Kitagawa, Daichi and Kobatake, Seiya and Miyasaka, Hiroshi
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2022): 175-184
Authors: Ito, Syoji and Hiratsuka, Kengo and Takei, Satoshi and Nishi, Hiroyasu and Kitagawa, Daichi and Kobatake, Seiya and Miyasaka, Hiroshi
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2022): 175-184
Atto488-Agitoxin 2-A Fluorescent Ligand with Increased Selectivity for Kv1.3 Channel Binding Site.
Authors: Denisova, Kristina R and Orlov, Nikita A and Yakimov, Sergey A and Kirpichnikov, Mikhail P and Feofanov, Alexey V and Nekrasova, Oksana V
Journal: Bioengineering (Basel, Switzerland) (2022)
Authors: Denisova, Kristina R and Orlov, Nikita A and Yakimov, Sergey A and Kirpichnikov, Mikhail P and Feofanov, Alexey V and Nekrasova, Oksana V
Journal: Bioengineering (Basel, Switzerland) (2022)
Lipophilic probe behavior in microemulsions evaluated by fluorescence correlation spectroscopy.
Authors: Yamamoto, Johtaro and Suzuki, Yoshio and Ogawa, Yoshikatsu and Kamata, Tomoyuki and Hashimoto, Hinako and Kunitake, Masashi and Kato, Dai
Journal: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry (2022): 401-408
Authors: Yamamoto, Johtaro and Suzuki, Yoshio and Ogawa, Yoshikatsu and Kamata, Tomoyuki and Hashimoto, Hinako and Kunitake, Masashi and Kato, Dai
Journal: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry (2022): 401-408
A Model of F-actin Organization in Granuloreticulopodia in Foraminifera: Morphogenetic and Evolutionary Implications from Novel Fluorescent and Polarised Light Observations.
Authors: Goleń, Jan and Tyszka, Jarosław and Godos, Karolina and Janse, Max
Journal: Protist (2022): 125886
Authors: Goleń, Jan and Tyszka, Jarosław and Godos, Karolina and Janse, Max
Journal: Protist (2022): 125886
Evaluation of [Cys(ATTO 488)8]Dermorphin-NH2 as a novel tool for the study of μ-opioid peptide receptors.
Authors: Giakomidi, Despina and Bird, Mark F and McDonald, John and Marzola, Erika and Guerrini, Remo and Chanoch, Serena and Sabu, Nidhuna and Horley, Barbara and Calo, Girolamo and Lambert, David G
Journal: PloS one (2021): e0250011
Authors: Giakomidi, Despina and Bird, Mark F and McDonald, John and Marzola, Erika and Guerrini, Remo and Chanoch, Serena and Sabu, Nidhuna and Horley, Barbara and Calo, Girolamo and Lambert, David G
Journal: PloS one (2021): e0250011
Multimodal fluorescently labeled polymer-coated GdF3 nanoparticles inhibit degranulation in mast cells.
Authors: Shapoval, Oleksandr and Sulimenko, Vadym and Klebanovych, Anastasiya and Rabyk, Mariia and Shapoval, Pavlo and Kaman, Ondřej and Rydvalová, Eliška and Filipová, Marcela and Dráberová, Eduarda and Dráber, Pavel and Horák, Daniel
Journal: Nanoscale (2021): 19023-19037
Authors: Shapoval, Oleksandr and Sulimenko, Vadym and Klebanovych, Anastasiya and Rabyk, Mariia and Shapoval, Pavlo and Kaman, Ondřej and Rydvalová, Eliška and Filipová, Marcela and Dráberová, Eduarda and Dráber, Pavel and Horák, Daniel
Journal: Nanoscale (2021): 19023-19037