ATTO 514 maleimide

ATTO 514 maleimide is a thiol‑reactive, green fluorescent dye with an excitation/emission of 510/531 nm that is used for FACS and FISH applications.

Selective Thiol Coupling: Chemoselective labeling of cysteine‑containing peptides, thiol‑modified oligonucleotides, and ligands
Superior Brightness & Photostability: Supports long‑term imaging and demanding light exposure
Moderate Hydrophilicity: Minimizes aggregation while preserving compatibility with aqueous buffers

Chemical structure for ATTO 514 maleimide.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
2864	1 mg	Price

Overview

ATTO 514 maleimide is manufactured by AAT Bioquest for research and development use. ATTO 514 is a rhodamine-based fluorescent labeling dye known for its favorable spectral qualities. With a high molar extinction coefficient, a fluorescence quantum yield of 0.85, high photo-stability, and excellent water solubility, it is an ideal choice for single-molecule detection, high-resolution microscopy (e.g., SIM and STED), flow cytometry, and fluorescence in-situ hybridization (FISH). It exhibits its highest fluorescence efficiency when excited within the range of 510-535 nm, making it a perfect match for the 514 nm line of an argon-ion laser. For labeling antibodies and other proteins, we highly recommend our iFluor® dyes that are highly water soluble and are optimized for labeling proteins.ATTO 514 maleimide is used for labeling thiol-containing molecules, such as peptides and oligonucleotides.

References

View all 50 references: Citation Explorer

Ratiometric Fluorescence Detection of Colorectal Cancer-Associated Exosomal miR-92a-3p with DSN-Assisted Signal Amplification by a MWCNTs@Au NCs Nanoplatform.

Authors:

Sun, Zhiwei and Li, Juan and Tong, Yao and Zhao, Li and Zhou, Xiaoyu and Li, Hui and Wang, Chuanxin and Du, Lutao and Jiang, Yanyan

Journal:

Biosensors (2022)

Development of Fluorescence-Tagged SARS-CoV-2 Virus-like Particles by a Tri-Cistronic Vector Expression System for Investigating the Cellular Entry of SARS-CoV-2.

Authors:

Chang, Young-Sheng and Chu, Li-Wei and Chen, Zan-Yu and Wu, Joh-Sin and Su, Wen-Chi and Yang, Chia-Jui and Ping, Yueh-Hsin and Lin, Cheng-Wen

Journal:

Viruses (2022)

Lipophilic probe behavior in microemulsions evaluated by fluorescence correlation spectroscopy.

Authors:

Yamamoto, Johtaro and Suzuki, Yoshio and Ogawa, Yoshikatsu and Kamata, Tomoyuki and Hashimoto, Hinako and Kunitake, Masashi and Kato, Dai

Journal:

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry (2022): 401-408

Scanning Two-Dimensional Fluorescence Lifetime Correlation Spectroscopy: Conformational Dynamics of DNA Holliday Junction from Microsecond to Subsecond.

Authors:

Heo, Wooseok and Hasegawa, Kazuto and Okamoto, Kenji and Sako, Yasushi and Ishii, Kunihiko and Tahara, Tahei

Journal:

The journal of physical chemistry letters (2022): 1249-1257

Two-Photon-Excited Single-Molecule Fluorescence Enhanced by Gold Nanorod Dimers.

Authors:

Lu, Xuxing and Punj, Deep and Orrit, Michel

Journal:

Nano letters (2022): 4215-4222

Physical properties

Molecular weight	875.77
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.21
Correction factor (280 nm)	0.08
Extinction coefficient (cm ^-1 M ^-1)	115,000
Excitation (nm)	510
Emission (nm)	531
Quantum yield	0.85

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 15, 2025