APC/Cy7 Anti-human CD14 Antibody *61D3*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Spectral properties
Extinction coefficient (cm -1 M -1) | 700000 |
Excitation (nm) | 651 |
Emission (nm) | 779 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Overview | SDSProtocol |
Other names LPS-Receptor | Clone 61D3 | Host mouse | Reactivity human | Application | Extinction coefficient (cm -1 M -1) 700000 | Excitation (nm) 651 | Emission (nm) 779 |
61D3 is an anti-human monoclonal antibody that targets the CD14 antigen. CD14 (sometimes referred to as myeloid cell-specific leucine-rich glycoprotein or LPS receptor) is a transmembrane protein that is located on the surface of cells such as macrophages. CD14 acts in critical cellular pathways, for example, the toll-like receptor signaling pathway, cell surface receptor signaling pathway and lipopolysaccharide-mediated signaling pathway. Moreover, in certain organisms, it promotes interleukin-8 secretion, is a positive regulator of tumor necrosis factor production and is a promoter of type I interferon production. From a research standpoint, it is of biological interest due to its association with essential macromolecules/ligands like LY96. CD14 is a very popular antibody target, with over 42000 publications in the last decade. CD14 is essential for immunology, cell biology and neuroscience research, often serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to APC/Cy7 (ex/em = 754/779 nm).
Spectrum
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Spectral properties
Extinction coefficient (cm -1 M -1) | 700000 |
Excitation (nm) | 651 |
Emission (nm) | 779 |
Antibody Finder
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Conjugation
We provide custom conjugation services for this antibody (eg. labeling of cd14 antibody with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
Proteins | HRP, Alkaline Phosphatase, Streptavidin |
Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
Small Molecules | Biotin |
Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
FAQ
How can I lyse my cells without lysing the nuclear membrane?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?