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Why are black dots appearing on my western blot?
Posted June 24, 2024
Antibodies and Proteomics BioconjugationAntibodies and ProteomicsWestern Blot
Answer

There are several reasons why black dots appear on western blots. One reason is because antibodies may bind to the blocking solution. This can happen if the blocking reagent has clumped together allowing the antibodies to bind to it resulting in positive signal dots. 

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