When should I use qPCR for DNA library normalization?

Posted June 19, 2024

DNA and RNA Quantitation

Polymerase Chain Reaction (PCR)

Real-Time PCR (qPCR)

Sanger Sequencing

gene sequencing

Answer

Use qPCR for DNA library normalization in these situations:

Your samples are valuable, available in limited quality, or originate from varied sources
You are looking for the ultimate accuracy for normalization with no variations in quantitation and read depth
Ensuring a minimum target read depth is of utmost importance

Additional resources

Comparison between magnetic bead and qPCR library normalisation methods for forensic MPS genotyping

Real-Time PCR (qPCR)

Helixyte™ Green *20X Aqueous PCR Solution*

Related questions

What is the typical workflow of DNA quantification and normalization?

Is there alternative techniques to Southern blotting?

What are the advantages of second generation NGS?

What are the advantages of third generation NGS?

What are the disadvantages of second generation NGS?