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AAT Bioquest

When should I use qPCR for DNA library normalization?

Posted June 19, 2024


Answer

Use qPCR for DNA library normalization in these situations: 

  • Your samples are valuable, available in limited quality, or originate from varied sources
  • You are looking for the ultimate accuracy for normalization with no variations in quantitation and read depth
  • Ensuring a minimum target read depth is of utmost importance
Additional resources

Comparison between magnetic bead and qPCR library normalisation methods for forensic MPS genotyping

Real-Time PCR (qPCR)

Helixyte™ Green *20X Aqueous PCR Solution*