What is the typical workflow of DNA quantification and normalization?

Posted June 19, 2024

DNA and RNA Quantitation

Polymerase Chain Reaction (PCR)

Real-Time PCR (qPCR)

Sanger Sequencing

gene sequencing

Answer

The typical workflow of DNA quantification and normalization can be encapsulated in 4 steps:

Set up quantification assay
Determine DNA concentration
Calculate a dilution scheme with the aim of achieving the target concentration for each well
Dilute DNA samples to the desired concentration

Additional resources

The Illumina Sequencing Protocol and the NovaSeq 6000 System

DNA and RNA Quantitation

DNA Concentration Calculator

Helixyte™ Green dsDNA Quantifying Reagent *200X DMSO Solution*

Related questions

When should I use qPCR for DNA library normalization?

Is there alternative techniques to Southern blotting?

What are the advantages of second generation NGS?

What are the advantages of third generation NGS?

What are the disadvantages of second generation NGS?