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What is the typical workflow of DNA quantification and normalization?

Posted June 19, 2024


DNA and RNA Quantitation
Polymerase Chain Reaction (PCR)
Real-Time PCR (qPCR)
Sanger Sequencing
gene sequencing
Answer

The typical workflow of DNA quantification and normalization can be encapsulated in 4 steps: 

  1. Set up quantification assay
  2. Determine DNA concentration
  3. Calculate a dilution scheme with the aim of achieving the target concentration for each well
  4. Dilute DNA samples to the desired concentration
Additional resources

The Illumina Sequencing Protocol and the NovaSeq 6000 System

DNA and RNA Quantitation

DNA Concentration Calculator

Helixyte™ Green dsDNA Quantifying Reagent *200X DMSO Solution*

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