What materials are needed to make a phosphate buffer?

Posted April 26, 2023

Chemical Reagents

Buffer Preparations and Recipes

Answer

The two main materials needed to make a phosphate buffer are:

Sodium Phosphate Dibasic Heptahydrate
Sodium Phosphate Monobasic Monohydrate

Other materials needed are:

Phosphoric acid (to make the pH more acidic) or Sodium hydroxide (to make the pH more alkaline)
Distilled Water
pH meter
Hot plate with stirring bar
Glassware

Additional resources

The use of phosphate buffered saline for the recovery of cells and spermatozoa from swabs

Phosphate Buffer (pH 5.8 to 7.4) Preparation and Recipe

Buffers and Lab Consumables

Related questions

Does adding triton with the primary antibody hinder antigen detection in immunohistochemistry?

Does PBS buffer have any advantage over SSC buffer?

Does PBS buffer have any effect on cell adhesion?

For primer dilution in PCR, should I use water or TE buffer?

How can you detect endogenous expression of early endosome and lysosome in immunofluorescence?