What is the function of adding BSA as buffer in Biotin-Streptavidin system?
Posted April 17, 2020
Answer
The function of BSA in biotin-streptavidin systems is to reduce non-specific binding and background as a blocking agent. Milk is generally a commonly used blocking buffer but is not recommended to use with biotin systems since the biotin found in milk can interfere with the assay.
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