What is the recommended sequencing depth to quantify CRISPR editing?

The recommended sequencing depth to quantify CRISPR editing is 1000 paired-end reads per amplicon. For example, detecting cancer mutations present at low fractions requires high sequencing coverage. To confidently detect single nucleotide variants (SNVs) present at a 5% fraction, an average sequencing depth of over 1000 reads is typically necessary. Even higher sequencing depth is required to reliably detect SNVs at fractions lower than 5%.