What is the major difference in RNA processing between prokaryotes and eukaryotes?

The major difference in RNA processing between prokaryotes and eukaryotes is the processing of messenger RNAs. Eukaryotes splice mainly protein-coding messenger RNAs and some non-coding RNAs. Transcription occurs in the nucleus and does not occur simultaneously with translation. In prokaryotic cells, transcription takes place in the cytoplasm (in tRNAs and non-coding RNAs) and at the same time as translation.