logo
AAT Bioquest

 
search
logo
AAT Bioquest
Contact us
Company
Telephone:1-408-733-1055
Fax:1-408-733-1304
Hours:Monday to Friday
8:30 - 17:30 PST (GMT-8)
Location:5775 W Las Positas Blvd.
Pleasanton, CA, 94588
USA

Email us
Sales:sales@aatbio.com
Technical Support:support@aatbio.com
Website:websupport@aatbio.com
General Inquiries:info@aatbio.com
Order info
United States of America
Telephone:1-408-733-1055
Fax:1-408-733-1304
Email:sales@aatbio.com
Purchase Order:sales@aatbio.com

International
Click here to see all available distributors
 
search
calculatorCalculator

What is the difference between Firefly and Renilla Luciferase?

Posted July 14, 2022


Enzymes
Luciferases
Cell Metabolism
Cellular Processes
Answer

Firefly luciferase assay uses luciferin in the presence of oxygen, ATP and magnesium to produce Green/Yellow light of 550-70 nm. Renilla luciferase assay requires only coelenterazine and oxygen to produce Blue light of 480 nm. 

Additional resources

Optical imaging of Renilla luciferase, synthetic Renilla luciferase, and firefly luciferase reporter gene expression in living mice

Luciferases

Amplite® Gaussia Luciferase Reporter Gene Assay Kit *Maximized Luminescence*

Related questions
What are the advantages of dual luciferase assays?
What are the applications of luciferase assays?
What are the differences between firefly luciferase and Gaussia luciferase?
What is a dual luciferase assay?
What is luciferase?