Posted July 11, 2022
Answer
Sanger sequencing requires:
- A DNA template
- A sequencing primer, which is a short piece of DNA
- A thermostable DNA polymerase, which is the enzyme that copies DNA
- Nucleotides (dNTPS)
- dideoxynucleotides (ddNTPs)
- Buffer
Additional resources
A simplified Sanger sequencing method for full genome sequencing of multiple subtypes of human influenza A viruses
MagaDye™ 4 Color Sanger Sequencing Terminator Kit
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