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What is acceptor photobleaching?
Posted May 15, 2024
Answer
Acceptor photobleaching is a technique used in FRET experiments to confirm the occurrence of energy transfer between a donor and acceptor fluorophore pair. The rise in donor fluorescence following complete acceptor photobleaching serves as an indicator of the extent of FRET.
Additional resources
Fluorescence resonance energy transfer (FRET) measurement by gradual acceptor photobleaching
Förster Resonance Energy Transfer (FRET)