How does acceptor photobleaching work?
Posted May 15, 2024
Answer
In this method, the acceptor fluorophores are selectively bleached (irreversibly destroyed) using intense light, which causes them to lose their ability to accept energy from the donor. If energy transfer was indeed occurring before bleaching, the loss of acceptor fluorescence should lead to an increase in donor fluorescence intensity.
Additional resources
Fluorescence resonance energy transfer (FRET) measurement by gradual acceptor photobleaching
Förster Resonance Energy Transfer (FRET)