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AAT Bioquest

How does acceptor photobleaching work?

Posted May 15, 2024


Answer

In this method, the acceptor fluorophores are selectively bleached (irreversibly destroyed) using intense light, which causes them to lose their ability to accept energy from the donor. If energy transfer was indeed occurring before bleaching, the loss of acceptor fluorescence should lead to an increase in donor fluorescence intensity. 

Additional resources

Fluorescence resonance energy transfer (FRET) measurement by gradual acceptor photobleaching

Förster Resonance Energy Transfer (FRET)

Screen Quest™ TR-FRET No Wash cAMP Assay Kit

FMOC-Asp(5-FAM)-OH