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AAT Bioquest

What does short-read sequencing mean?

Posted June 4, 2024


Answer

Short-read sequencing is a type of next-generation sequencing (NGS). It involves breaking a genome into small fragments, usually 50 to 300 bases, before being sequenced. Short-read sequencing or SRS is a powerful tool for generating genomic data and is the most widely used form of NGS with a wide range of diagnostic applications. It is accurate, cost-effective and fast, capable of sequencing the entire genome in roughly one day. 

Additional resources

Mapping short DNA sequencing reads and calling variants using mapping quality scores

Next Generation Sequencing (NGS)

MagaDye™ 535-ddGTP