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AAT Bioquest

What are the steps of atomic absorption spectroscopy?

Posted July 25, 2024


Answer

To perform atomic absorption spectroscopy:

  • The liquid sample is mixed with a specific volume of spirit and added to a flask.
  • A lamp with the necessary wavelength is set as a light source. 
  • A fuel-rich acetylene-nitrous oxide flame is used to vaporize the liquid sample into a gas. 
  • The gas is passed through a detector that is designed to detect the absorbance of the atoms in the gas. 
  • The absorbance of the solvent bank and standard solution is detected using a similar process. 
  • A graph is plotted showing the absorbance against the molecule concentration in the sample. 
Additional resources

Flameless atomic absorption spectroscopy of plasma chromium

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